EMB2279 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMB2279
Entrez Gene ID	839941
Full Name	pentatricopeptide (PPR) repeat-containing protein
Synonyms	EMB88,EMBRYO DEFECTIVE 2279,EMBRYO DEFECTIVE 88,T5I8.6,T5I8_6
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_102798.2	NP_174349.1	pentatricopeptide (PPR) repeat-containing protein
NM_001198188.2	NP_001185117.1	pentatricopeptide (PPR) repeat-containing protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Theologis A, Ecker JR, Palm CJ, Federspiel NA, Kaul S, White O, Alonso J, Altafi H, Araujo R, Bowman CL, Brooks SY, Buehler E, Chan A, Chao Q, Chen H, Cheuk RF, Chin CW, Chung MK, Conn L, Conway AB, Conway AR, Creasy TH, Dewar K, Dunn P, Etgu P, Feldblyum TV, Feng J, Fong B, Fujii CY, Gill JE, Goldsmith AD, Haas B, Hansen NF, Hughes B, Huizar L, Hunter JL, Jenkins J, Johnson-Hopson C, Khan S, Khaykin E, Kim CJ, Koo HL, Kremenetskaia I, Kurtz DB, Kwan A, Lam B, Langin-Hooper S, Lee A, Lee JM, Lenz CA, Li JH, Li Y, Lin X, Liu SX, Liu ZA, Luros JS, Maiti R, Marziali A, Militscher J, Miranda M, Nguyen M, Nierman WC, Osborne BI, Pai G, Peterson J, Pham PK, Rizzo M, Rooney T, Rowley D, Sakano H, Salzberg SL, Schwartz JR, Shinn P, Southwick AM, Sun H, Tallon LJ, Tambunga G, Toriumi MJ, Town CD, Utterback T, Van Aken S, Vaysberg M, Vysotskaia VS, Walker M, Wu D, Yu G, Fraser CM, Venter JC, Davis RW
Nature408(6814)816-20(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EMB2279 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMB2279 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb25181	NM_001198188.2 Latest version!	Arabidopsis thaliana pentatricopeptide (PPR) repeat-containing protein (EMB2279), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OAb30436	NM_102798.2 Latest version!	Arabidopsis thaliana pentatricopeptide (PPR) repeat-containing protein (EMB2279), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMB2279 ( NM_001198188.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001185117.1 Arabidopsis thaliana pentatricopeptide (PPR) repeat-containing protein (EMB2279), mRNA.

Price & Availability↑

CloneID	OAb25181
Clone ID Related Accession (Same CDS sequence)	NM_001198188.2
Accession Version	NM_001198188.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	pentatricopeptide (PPR) repeat-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_001198188.1.

ATGGCGGTGA CGATTTCGAC GAATGCTTTC GTAAATGCAT CGCTTTTAGA TGAAAGTCGG 
AATTCTTTCT GGAGACCATT GTTTCATCAG CCATACTATA ATTGCCGACG AGTCGTTCGT 
CTTAATTCGA GGAAATTGAA TTCAAAGGTA ATGTTTTGCT TGAATTTGAA CACGAAGGAG 
GTTGGTTTGC AAAAACCCGG TGATAAAGGT TTTGAATTCA AACCCAGTTT TGATCAGTAC 
CTGCAAATCA TGGAATCGGT TAAAACAGCA AGGAAGAAGA AGAAATTCGA CAGATTGAAA 
GTTGAGGAAG ATGATGGTGG AGGTGGGAAT GGTGATAGTG TTTATGAAGT GAAAGATATG 
AAGATTAAGA GTGGTGAGCT AAAAGATGAA ACTTTCAGGA AGAGATACTC AAGGCAGGAG 
ATTGTAAGTG ATAAACGTAA TGAGAGAGTT TTCAAGAGGA ATGGAGAAAT TGAAAATCAT 
AGAGTGGCTA CAGATTTGAA ATGGAGTAAG AGTGGTGAAT CTTCAGTGGC TCTGAAATTG 
AGTAAGAGTG GTGAATCTTC AGTGACTGTG CCTGAAGATG AGAGTTTCAG GAAAAGGTAC 
TCTAAGCAGG AGTATCACCG TTCCTCTGAT ACATCGAGAG GGATTGAAAG AGGTTCGAGA 
GGTGATGAAT TGGATCTTGT TGTTGAAGAA AGGAGAGTTC AGAGAATAGC CAAAGATGCA 
AGATGGAGTA AAAGTCGTGA ATCTTCAGTG GCTGTGAAAT GGAGTAATAG TGGTGAATCT 
TCAGTGACTA TGCCTAAAGA TGAGAGCTTT AGGAGAAGAT ACTCTAAGCA GGAGCATCAC 
CGTTCCTCTG ATACATCCAG AGGGATTGCA AGAGGTTCAA AAGGTGATGA ATTGGAGCTT 
GTTGTTGAAG AAAGGAGAGT TCAGAGAATA GCCAAAGATG TAAGATGGAG TAAGAGTGAT 
GAATCTTTAG TGCCTGTGTC AGAAGATGAG AGTTTCAGGA GAGGGAATCC GAAGCAGGAG 
ATGGTGAGGT ATCAGCGTGT CTCTGATACA TCGAGAGGGA TTGAGAGAGG TTCCAAAGGA 
GATGGATTGG ATCTTCTTGC TGAAGAAAGG CGGATTGAGA GATTAGCCAA TGAGAGGCAT 
GAGATAAGAA GTAGCAAATT GAGTGGAACC AGGAGAATTG GTGCTAAGAG AAATGATGAT 
GATGATGATA GCTTGTTTGC CATGGAAACT CCTGCCTTTA GGTTTTCTGA TGAGTCCAGT 
GACATAGTGG ACAAGCCAGC TACTTCACGA GTCGAAATGG AAGACAGAAT CGAGAAGTTA 
GCAAAAGTGT TGAATGGTGC AGACATCAAT ATGCCTGAGT GGCAGTTTTC CAAGGCGATC 
AGGAGTGCAA AAATCAGATA TACGGATTAC ACAGTAATGA GACTGATCCA CTTTCTAGGG 
AAACTAGGAA ACTGGAGACG AGTTCTTCAA GTCATTGAGT GGCTTCAAAG GCAAGACCGT 
TACAAATCTA ACAAGATAAG AATCATCTAT ACAACTGCAC TAAATGTTCT TGGTAAATCA 
AGGAGGCCTG TGGAAGCTCT CAATGTATTC CACGCTATGC TGTTACAAAT TTCATCATAT 
CCGGATATGG TAGCATACCG TTCAATTGCA GTCACACTTG GACAAGCTGG GCATATCAAG 
GAACTCTTCT ATGTGATTGA CACAATGAGG TCTCCACCTA AAAAGAAGTT CAAGCCAACA 
ACACTTGAAA AATGGGATCC CCGGCTTGAA CCAGATGTTG TTGTTTACAA TGCGGTGCTC 
AACGCATGTG TTCAACGAAA GCAATGGGAA GGAGCATTCT GGGTATTGCA ACAGTTGAAG 
CAACGAGGGC AAAAACCTTC TCCTGTAACC TATGGCCTCA TCATGGAGGT AATGTTAGCA 
TGTGAGAAGT ACAATTTAGT TCATGAATTC TTCAGGAAGA TGCAGAAATC TTCTATCCCT 
AATGCTCTAG CATATAGAGT TCTTGTTAAT ACTCTATGGA AAGAAGGTAA AAGCGACGAG 
GCCGTACATA CGGTTGAGGA TATGGAAAGC CGTGGTATTG TTGGATCAGC TGCTCTTTAC 
TACGACCTTG CTCGCTGTCT ATGTAGCGCA GGAAGGTGTA ATGAAGGGCT CAATATGCTT 
AAGAAGATAT GTAGAGTTGC AAATAAACCT CTCGTGGTGA CTTACACTGG CCTGATCCAA 
GCATGCGTCG ACTCGGGAAA CATCAAGAAT GCAGCTTACA TCTTCGATCA GATGAAGAAG 
GTCTGCAGCC CTAACCTAGT CACTTGCAAC ATAATGCTAA AAGCTTATCT ACAAGGCGGA 
TTGTTTGAAG AAGCAAGGGA ACTTTTCCAG AAGATGTCAG AAGACGGAAA TCATATAAAA 
AACAGCTCGG ATTTCGAATC AAGAGTATTG CCAGACACGT ACACGTTCAA CACGATGCTA 
GACACGTGTG CTGAACAAGA AAAGTGGGAT GATTTTGGTT ATGCGTATCG GGAGATGTTG 
CGTCATGGAT ACCATTTCAA TGCGAAACGC CATCTCAGAA TGGTACTTGA AGCTAGCAGA 
GCAGGAAAGG AGGAGGTGAT GGAAGCGACA TGGGAACACA TGAGACGAAG TAATAGAATT 
CCGCCATCGC CTCTAATCAA AGAAAGATTC TTCAGGAAAC TCGAGAAAGG CGATCATATT 
TCGGCTATAT CATCTCTTGC TGATCTTAAT GGAAAAATTG AGGAGACTGA GTTACGAGCA 
TTTTCAACTT CCGCATGGTC CAGAGTCTTG TCCCGATTTG AGCAAGATTC AGTTTTGAGG 
TTAATGGACG ATGTGAACAG ACGCCTAGGT TCGAGAAGTG AGTCTTCGGA TTCGGTTTTG 
GGGAATCTAT TGAGTTCTTG TAAAGATTAT CTGAAGACCA GAACACATAA CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185117.1
CDS	164..3100
Translation	MAVTISTNAFVNASLLDESRNSFWRPLFHQPYYNCRRVVRLNSRKLNSKVMFCLNLNTKEVGLQKPGDKGFEFKPSFDQYLQIMESVKTARKKKKFDRLKVEEDDGGGGNGDSVYEVKDMKIKSGELKDETFRKRYSRQEIVSDKRNERVFKRNGEIENHRVATDLKWSKSGESSVALKLSKSGESSVTVPEDESFRKRYSKQEYHRSSDTSRGIERGSRGDELDLVVEERRVQRIAKDARWSKSRESSVAVKWSNSGESSVTMPKDESFRRRYSKQEHHRSSDTSRGIARGSKGDELELVVEERRVQRIAKDVRWSKSDESLVPVSEDESFRRGNPKQEMVRYQRVSDTSRGIERGSKGDGLDLLAEERRIERLANERHEIRSSKLSGTRRIGAKRNDDDDDSLFAMETPAFRFSDESSDIVDKPATSRVEMEDRIEKLAKVLNGADINMPEWQFSKAIRSAKIRYTDYTVMRLIHFLGKLGNWRRVLQVIEWLQRQDRYKSNKIRIIYTTALNVLGKSRRPVEALNVFHAMLLQISSYPDMVAYRSIAVTLGQAGHIKELFYVIDTMRSPPKKKFKPTTLEKWDPRLEPDVVVYNAVLNACVQRKQWEGAFWVLQQLKQRGQKPSPVTYGLIMEVMLACEKYNLVHEFFRKMQKSSIPNALAYRVLVNTLWKEGKSDEAVHTVEDMESRGIVGSAALYYDLARCLCSAGRCNEGLNMLKKICRVANKPLVVTYTGLIQACVDSGNIKNAAYIFDQMKKVCSPNLVTCNIMLKAYLQGGLFEEARELFQKMSEDGNHIKNSSDFESRVLPDTYTFNTMLDTCAEQEKWDDFGYAYREMLRHGYHFNAKRHLRMVLEASRAGKEEVMEATWEHMRRSNRIPPSPLIKERFFRKLEKGDHISAISSLADLNGKIEETELRAFSTSAWSRVLSRFEQDSVLRLMDDVNRRLGSRSESSDSVLGNLLSSCKDYLKTRTHNL

Target ORF information:

RefSeq Version	NM_001198188.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana pentatricopeptide (PPR) repeat-containing protein (EMB2279), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198188.2

ORF Insert Sequence:

ATGGCGGTGA CGATTTCGAC GAATGCTTTC GTAAATGCAT CGCTTTTAGA TGAAAGTCGG 
AATTCTTTCT GGAGACCATT GTTTCATCAG CCATACTATA ATTGCCGACG AGTCGTTCGT 
CTTAATTCGA GGAAATTGAA TTCAAAGGTA ATGTTTTGCT TGAATTTGAA CACGAAGGAG 
GTTGGTTTGC AAAAACCCGG TGATAAAGGT TTTGAATTCA AACCCAGTTT TGATCAGTAC 
CTGCAAATCA TGGAATCGGT TAAAACAGCA AGGAAGAAGA AGAAATTCGA CAGATTGAAA 
GTTGAGGAAG ATGATGGTGG AGGTGGGAAT GGTGATAGTG TTTATGAAGT GAAAGATATG 
AAGATTAAGA GTGGTGAGCT AAAAGATGAA ACTTTCAGGA AGAGATACTC AAGGCAGGAG 
ATTGTAAGTG ATAAACGTAA TGAGAGAGTT TTCAAGAGGA ATGGAGAAAT TGAAAATCAT 
AGAGTGGCTA CAGATTTGAA ATGGAGTAAG AGTGGTGAAT CTTCAGTGGC TCTGAAATTG 
AGTAAGAGTG GTGAATCTTC AGTGACTGTG CCTGAAGATG AGAGTTTCAG GAAAAGGTAC 
TCTAAGCAGG AGTATCACCG TTCCTCTGAT ACATCGAGAG GGATTGAAAG AGGTTCGAGA 
GGTGATGAAT TGGATCTTGT TGTTGAAGAA AGGAGAGTTC AGAGAATAGC CAAAGATGCA 
AGATGGAGTA AAAGTCGTGA ATCTTCAGTG GCTGTGAAAT GGAGTAATAG TGGTGAATCT 
TCAGTGACTA TGCCTAAAGA TGAGAGCTTT AGGAGAAGAT ACTCTAAGCA GGAGCATCAC 
CGTTCCTCTG ATACATCCAG AGGGATTGCA AGAGGTTCAA AAGGTGATGA ATTGGAGCTT 
GTTGTTGAAG AAAGGAGAGT TCAGAGAATA GCCAAAGATG TAAGATGGAG TAAGAGTGAT 
GAATCTTTAG TGCCTGTGTC AGAAGATGAG AGTTTCAGGA GAGGGAATCC GAAGCAGGAG 
ATGGTGAGGT ATCAGCGTGT CTCTGATACA TCGAGAGGGA TTGAGAGAGG TTCCAAAGGA 
GATGGATTGG ATCTTCTTGC TGAAGAAAGG CGGATTGAGA GATTAGCCAA TGAGAGGCAT 
GAGATAAGAA GTAGCAAATT GAGTGGAACC AGGAGAATTG GTGCTAAGAG AAATGATGAT 
GATGATGATA GCTTGTTTGC CATGGAAACT CCTGCCTTTA GGTTTTCTGA TGAGTCCAGT 
GACATAGTGG ACAAGCCAGC TACTTCACGA GTCGAAATGG AAGACAGAAT CGAGAAGTTA 
GCAAAAGTGT TGAATGGTGC AGACATCAAT ATGCCTGAGT GGCAGTTTTC CAAGGCGATC 
AGGAGTGCAA AAATCAGATA TACGGATTAC ACAGTAATGA GACTGATCCA CTTTCTAGGG 
AAACTAGGAA ACTGGAGACG AGTTCTTCAA GTCATTGAGT GGCTTCAAAG GCAAGACCGT 
TACAAATCTA ACAAGATAAG AATCATCTAT ACAACTGCAC TAAATGTTCT TGGTAAATCA 
AGGAGGCCTG TGGAAGCTCT CAATGTATTC CACGCTATGC TGTTACAAAT TTCATCATAT 
CCGGATATGG TAGCATACCG TTCAATTGCA GTCACACTTG GACAAGCTGG GCATATCAAG 
GAACTCTTCT ATGTGATTGA CACAATGAGG TCTCCACCTA AAAAGAAGTT CAAGCCAACA 
ACACTTGAAA AATGGGATCC CCGGCTTGAA CCAGATGTTG TTGTTTACAA TGCGGTGCTC 
AACGCATGTG TTCAACGAAA GCAATGGGAA GGAGCATTCT GGGTATTGCA ACAGTTGAAG 
CAACGAGGGC AAAAACCTTC TCCTGTAACC TATGGCCTCA TCATGGAGGT AATGTTAGCA 
TGTGAGAAGT ACAATTTAGT TCATGAATTC TTCAGGAAGA TGCAGAAATC TTCTATCCCT 
AATGCTCTAG CATATAGAGT TCTTGTTAAT ACTCTATGGA AAGAAGGTAA AAGCGACGAG 
GCCGTACATA CGGTTGAGGA TATGGAAAGC CGTGGTATTG TTGGATCAGC TGCTCTTTAC 
TACGACCTTG CTCGCTGTCT ATGTAGCGCA GGAAGGTGTA ATGAAGGGCT CAATATGCTT 
AAGAAGATAT GTAGAGTTGC AAATAAACCT CTCGTGGTGA CTTACACTGG CCTGATCCAA 
GCATGCGTCG ACTCGGGAAA CATCAAGAAT GCAGCTTACA TCTTCGATCA GATGAAGAAG 
GTCTGCAGCC CTAACCTAGT CACTTGCAAC ATAATGCTAA AAGCTTATCT ACAAGGCGGA 
TTGTTTGAAG AAGCAAGGGA ACTTTTCCAG AAGATGTCAG AAGACGGAAA TCATATAAAA 
AACAGCTCGG ATTTCGAATC AAGAGTATTG CCAGACACGT ACACGTTCAA CACGATGCTA 
GACACGTGTG CTGAACAAGA AAAGTGGGAT GATTTTGGTT ATGCGTATCG GGAGATGTTG 
CGTCATGGAT ACCATTTCAA TGCGAAACGC CATCTCAGAA TGGTACTTGA AGCTAGCAGA 
GCAGGAAAGG AGGAGGTGAT GGAAGCGACA TGGGAACACA TGAGACGAAG TAATAGAATT 
CCGCCATCGC CTCTAATCAA AGAAAGATTC TTCAGGAAAC TCGAGAAAGG CGATCATATT 
TCGGCTATAT CATCTCTTGC TGATCTTAAT GGAAAAATTG AGGAGACTGA GTTACGAGCA 
TTTTCAACTT CCGCATGGTC CAGAGTCTTG TCCCGATTTG AGCAAGATTC AGTTTTGAGG 
TTAATGGACG ATGTGAACAG ACGCCTAGGT TCGAGAAGTG AGTCTTCGGA TTCGGTTTTG 
GGGAATCTAT TGAGTTCTTG TAAAGATTAT CTGAAGACCA GAACACATAA CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMB2279 ( NM_102798.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_174349.1 Arabidopsis thaliana pentatricopeptide (PPR) repeat-containing protein (EMB2279), partial mRNA.

Price & Availability↑

CloneID	OAb30436
Clone ID Related Accession (Same CDS sequence)	NM_102798.2
Accession Version	NM_102798.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	pentatricopeptide (PPR) repeat-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003070). On Sep 12, 2016 this sequence version replaced NM_102798.1. COMPLETENESS: incomplete on the 3' end.

ATGGCGGTGA CGATTTCGAC GAATGCTTTC GTAAATGCAT CGCTTTTAGA TGAAAGTCGG 
AATTCTTTCT GGAGACCATT GTTTCATCAG CCATACTATA ATTGCCGACG AGTCGTTCGT 
CTTAATTCGA GGAAATTGAA TTCAAAGGTA ATGTTTTGCT TGAATTTGAA CACGAAGGAG 
GTTGGTTTGC AAAAACCCGG TGATAAAGGT TTTGAATTCA AACCCAGTTT TGATCAGTAC 
CTGCAAATCA TGGAATCGGT TAAAACAGCA AGGAAGAAGA AGAAATTCGA CAGATTGAAA 
GTTGAGGAAG ATGATGGTGG AGGTGGGAAT GGTGATAGTG TTTATGAAGT GAAAGATATG 
AAGATTAAGA GTGGTGAGCT AAAAGATGAA ACTTTCAGGA AGAGATACTC AAGGCAGGAG 
ATTGTAAGTG ATAAACGTAA TGAGAGAGTT TTCAAGAGGA ATGGAGAAAT TGAAAATCAT 
AGAGTGGCTA CAGATTTGAA ATGGAGTAAG AGTGGTGAAT CTTCAGTGGC TCTGAAATTG 
AGTAAGAGTG GTGAATCTTC AGTGACTGTG CCTGAAGATG AGAGTTTCAG GAAAAGGTAC 
TCTAAGCAGG AGTATCACCG TTCCTCTGAT ACATCGAGAG GGATTGAAAG AGGTTCGAGA 
GGTGATGAAT TGGATCTTGT TGTTGAAGAA AGGAGAGTTC AGAGAATAGC CAAAGATGCA 
AGATGGAGTA AAAGTCGTGA ATCTTCAGTG GCTGTGAAAT GGAGTAATAG TGGTGAATCT 
TCAGTGACTA TGCCTAAAGA TGAGAGCTTT AGGAGAAGAT ACTCTAAGCA GGAGCATCAC 
CGTTCCTCTG ATACATCCAG AGGGATTGCA AGAGGTTCAA AAGGTGATGA ATTGGAGCTT 
GTTGTTGAAG AAAGGAGAGT TCAGAGAATA GCCAAAGATG TAAGATGGAG TAAGAGTGAT 
GAATCTTTAG TGCCTGTGTC AGAAGATGAG AGTTTCAGGA GAGGGAATCC GAAGCAGGAG 
ATGGTGAGGT ATCAGCGTGT CTCTGATACA TCGAGAGGGA TTGAGAGAGG TTCCAAAGGA 
GATGGATTGG ATCTTCTTGC TGAAGAAAGG CGGATTGAGA GATTAGCCAA TGAGAGGCAT 
GAGATAAGAA GTAGCAAATT GAGTGGAACC AGGAGAATTG GTGCTAAGAG AAATGATGAT 
GATGATGATA GCTTGTTTGC CATGGAAACT CCTGCCTTTA GGTTTTCTGA TGAGTCCAGT 
GACATAGTGG ACAAGCCAGC TACTTCACGA GTCGAAATGG AAGACAGAAT CGAGAAGTTA 
GCAAAAGTGT TGAATGGTGC AGACATCAAT ATGCCTGAGT GGCAGTTTTC CAAGGCGATC 
AGGAGTGCAA AAATCAGATA TACGGATTAC ACAGTAATGA GACTGATCCA CTTTCTAGGG 
AAACTAGGAA ACTGGAGACG AGTTCTTCAA GTCATTGAGT GGCTTCAAAG GCAAGACCGT 
TACAAATCTA ACAAGATAAG AATCATCTAT ACAACTGCAC TAAATGTTCT TGGTAAATCA 
AGGAGGCCTG TGGAAGCTCT CAATGTATTC CACGCTATGC TGTTACAAAT TTCATCATAT 
CCGGATATGG TAGCATACCG TTCAATTGCA GTCACACTTG GACAAGCTGG GCATATCAAG 
GAACTCTTCT ATGTGATTGA CACAATGAGG TCTCCACCTA AAAAGAAGTT CAAGCCAACA 
ACACTTGAAA AATGGGATCC CCGGCTTGAA CCAGATGTTG TTGTTTACAA TGCGGTGCTC 
AACGCATGTG TTCAACGAAA GCAATGGGAA GGAGCATTCT GGGTATTGCA ACAGTTGAAG 
CAACGAGGGC AAAAACCTTC TCCTGTAACC TATGGCCTCA TCATGGAGGT AATGTTAGCA 
TGTGAGAAGT ACAATTTAGT TCATGAATTC TTCAGGAAGA TGCAGAAATC TTCTATCCCT 
AATGCTCTAG CATATAGAGT TCTTGTTAAT ACTCTATGGA AAGAAGGTAA AAGCGACGAG 
GCCGTACATA CGGTTGAGGA TATGGAAAGC CGTGGTATTG TTGGATCAGC TGCTCTTTAC 
TACGACCTTG CTCGCTGTCT ATGTAGCGCA GGAAGGTGTA ATGAAGGGCT CAATATGGTA 
AATTTTGTAA ATCCTGTAGT CCTTAAGCTT ATCGAGAATT TGATTTACAA AGCTGATCTT 
GTTCATACCA TCCAATTTCA GCTTAAGAAG ATATGTAGAG TTGCAAATAA ACCTCTCGTG 
GTGACTTACA CTGGCCTGAT CCAAGCATGC GTCGACTCGG GAAACATCAA GAATGCAGCT 
TACATCTTCG ATCAGATGAA GAAGGTCTGC AGCCCTAACC TAGTCACTTG CAACATAATG 
CTAAAAGCTT ATCTACAAGG CGGATTGTTT GAAGAAGCAA GGGAACTTTT CCAGAAGATG 
TCAGAAGACG GAAATCATAT AAAAAACAGC TCGGATTTCG AATCAAGAGT ATTGCCAGAC 
ACGTACACGT TCAACACGAT GCTAGACACG TGTGCTGAAC AAGAAAAGTG GGATGATTTT 
GGTTATGCGT ATCGGGAGAT GTTGCGTCAT GGATACCATT TCAATGCGAA ACGCCATCTC 
AGAATGGTAC TTGAAGCTAG CAGAGCAGGA AAGGAGGAGG TGATGGAAGC GACATGGGAA 
CACATGAGAC GAAGTAATAG AATTCCGCCA TCGCCTCTAA TCAAAGAAAG ATTCTTCAGG 
AAACTCGAGA AAGGCGATCA TATTTCGGCT ATATCATCTC TTGCTGATCT TAATGGAAAA 
ATTGAGGAGA CTGAGTTACG AGCATTTTCA ACTTCCGCAT GGTCCAGAGT CTTGTCCCGA 
TTTGAGCAAG ATTCAGTTTT GAGGTTAATG GACGATGTGA ACAGACGCCT AGGTTCGAGA 
AGTGAGTCTT CGGATTCGGT TTTGGGGAAT CTATTGAGTT CTTGTAAAGA TTATCTGAAG 
ACCAGAACAC ATAACTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_174349.1
CDS	134..3154
Translation	MAVTISTNAFVNASLLDESRNSFWRPLFHQPYYNCRRVVRLNSRKLNSKVMFCLNLNTKEVGLQKPGDKGFEFKPSFDQYLQIMESVKTARKKKKFDRLKVEEDDGGGGNGDSVYEVKDMKIKSGELKDETFRKRYSRQEIVSDKRNERVFKRNGEIENHRVATDLKWSKSGESSVALKLSKSGESSVTVPEDESFRKRYSKQEYHRSSDTSRGIERGSRGDELDLVVEERRVQRIAKDARWSKSRESSVAVKWSNSGESSVTMPKDESFRRRYSKQEHHRSSDTSRGIARGSKGDELELVVEERRVQRIAKDVRWSKSDESLVPVSEDESFRRGNPKQEMVRYQRVSDTSRGIERGSKGDGLDLLAEERRIERLANERHEIRSSKLSGTRRIGAKRNDDDDDSLFAMETPAFRFSDESSDIVDKPATSRVEMEDRIEKLAKVLNGADINMPEWQFSKAIRSAKIRYTDYTVMRLIHFLGKLGNWRRVLQVIEWLQRQDRYKSNKIRIIYTTALNVLGKSRRPVEALNVFHAMLLQISSYPDMVAYRSIAVTLGQAGHIKELFYVIDTMRSPPKKKFKPTTLEKWDPRLEPDVVVYNAVLNACVQRKQWEGAFWVLQQLKQRGQKPSPVTYGLIMEVMLACEKYNLVHEFFRKMQKSSIPNALAYRVLVNTLWKEGKSDEAVHTVEDMESRGIVGSAALYYDLARCLCSAGRCNEGLNMVNFVNPVVLKLIENLIYKADLVHTIQFQLKKICRVANKPLVVTYTGLIQACVDSGNIKNAAYIFDQMKKVCSPNLVTCNIMLKAYLQGGLFEEARELFQKMSEDGNHIKNSSDFESRVLPDTYTFNTMLDTCAEQEKWDDFGYAYREMLRHGYHFNAKRHLRMVLEASRAGKEEVMEATWEHMRRSNRIPPSPLIKERFFRKLEKGDHISAISSLADLNGKIEETELRAFSTSAWSRVLSRFEQDSVLRLMDDVNRRLGSRSESSDSVLGNLLSSCKDYLKTRTHNL

Target ORF information:

RefSeq Version	NM_102798.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana pentatricopeptide (PPR) repeat-containing protein (EMB2279), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_102798.2

ORF Insert Sequence:

ATGGCGGTGA CGATTTCGAC GAATGCTTTC GTAAATGCAT CGCTTTTAGA TGAAAGTCGG 
AATTCTTTCT GGAGACCATT GTTTCATCAG CCATACTATA ATTGCCGACG AGTCGTTCGT 
CTTAATTCGA GGAAATTGAA TTCAAAGGTA ATGTTTTGCT TGAATTTGAA CACGAAGGAG 
GTTGGTTTGC AAAAACCCGG TGATAAAGGT TTTGAATTCA AACCCAGTTT TGATCAGTAC 
CTGCAAATCA TGGAATCGGT TAAAACAGCA AGGAAGAAGA AGAAATTCGA CAGATTGAAA 
GTTGAGGAAG ATGATGGTGG AGGTGGGAAT GGTGATAGTG TTTATGAAGT GAAAGATATG 
AAGATTAAGA GTGGTGAGCT AAAAGATGAA ACTTTCAGGA AGAGATACTC AAGGCAGGAG 
ATTGTAAGTG ATAAACGTAA TGAGAGAGTT TTCAAGAGGA ATGGAGAAAT TGAAAATCAT 
AGAGTGGCTA CAGATTTGAA ATGGAGTAAG AGTGGTGAAT CTTCAGTGGC TCTGAAATTG 
AGTAAGAGTG GTGAATCTTC AGTGACTGTG CCTGAAGATG AGAGTTTCAG GAAAAGGTAC 
TCTAAGCAGG AGTATCACCG TTCCTCTGAT ACATCGAGAG GGATTGAAAG AGGTTCGAGA 
GGTGATGAAT TGGATCTTGT TGTTGAAGAA AGGAGAGTTC AGAGAATAGC CAAAGATGCA 
AGATGGAGTA AAAGTCGTGA ATCTTCAGTG GCTGTGAAAT GGAGTAATAG TGGTGAATCT 
TCAGTGACTA TGCCTAAAGA TGAGAGCTTT AGGAGAAGAT ACTCTAAGCA GGAGCATCAC 
CGTTCCTCTG ATACATCCAG AGGGATTGCA AGAGGTTCAA AAGGTGATGA ATTGGAGCTT 
GTTGTTGAAG AAAGGAGAGT TCAGAGAATA GCCAAAGATG TAAGATGGAG TAAGAGTGAT 
GAATCTTTAG TGCCTGTGTC AGAAGATGAG AGTTTCAGGA GAGGGAATCC GAAGCAGGAG 
ATGGTGAGGT ATCAGCGTGT CTCTGATACA TCGAGAGGGA TTGAGAGAGG TTCCAAAGGA 
GATGGATTGG ATCTTCTTGC TGAAGAAAGG CGGATTGAGA GATTAGCCAA TGAGAGGCAT 
GAGATAAGAA GTAGCAAATT GAGTGGAACC AGGAGAATTG GTGCTAAGAG AAATGATGAT 
GATGATGATA GCTTGTTTGC CATGGAAACT CCTGCCTTTA GGTTTTCTGA TGAGTCCAGT 
GACATAGTGG ACAAGCCAGC TACTTCACGA GTCGAAATGG AAGACAGAAT CGAGAAGTTA 
GCAAAAGTGT TGAATGGTGC AGACATCAAT ATGCCTGAGT GGCAGTTTTC CAAGGCGATC 
AGGAGTGCAA AAATCAGATA TACGGATTAC ACAGTAATGA GACTGATCCA CTTTCTAGGG 
AAACTAGGAA ACTGGAGACG AGTTCTTCAA GTCATTGAGT GGCTTCAAAG GCAAGACCGT 
TACAAATCTA ACAAGATAAG AATCATCTAT ACAACTGCAC TAAATGTTCT TGGTAAATCA 
AGGAGGCCTG TGGAAGCTCT CAATGTATTC CACGCTATGC TGTTACAAAT TTCATCATAT 
CCGGATATGG TAGCATACCG TTCAATTGCA GTCACACTTG GACAAGCTGG GCATATCAAG 
GAACTCTTCT ATGTGATTGA CACAATGAGG TCTCCACCTA AAAAGAAGTT CAAGCCAACA 
ACACTTGAAA AATGGGATCC CCGGCTTGAA CCAGATGTTG TTGTTTACAA TGCGGTGCTC 
AACGCATGTG TTCAACGAAA GCAATGGGAA GGAGCATTCT GGGTATTGCA ACAGTTGAAG 
CAACGAGGGC AAAAACCTTC TCCTGTAACC TATGGCCTCA TCATGGAGGT AATGTTAGCA 
TGTGAGAAGT ACAATTTAGT TCATGAATTC TTCAGGAAGA TGCAGAAATC TTCTATCCCT 
AATGCTCTAG CATATAGAGT TCTTGTTAAT ACTCTATGGA AAGAAGGTAA AAGCGACGAG 
GCCGTACATA CGGTTGAGGA TATGGAAAGC CGTGGTATTG TTGGATCAGC TGCTCTTTAC 
TACGACCTTG CTCGCTGTCT ATGTAGCGCA GGAAGGTGTA ATGAAGGGCT CAATATGGTA 
AATTTTGTAA ATCCTGTAGT CCTTAAGCTT ATCGAGAATT TGATTTACAA AGCTGATCTT 
GTTCATACCA TCCAATTTCA GCTTAAGAAG ATATGTAGAG TTGCAAATAA ACCTCTCGTG 
GTGACTTACA CTGGCCTGAT CCAAGCATGC GTCGACTCGG GAAACATCAA GAATGCAGCT 
TACATCTTCG ATCAGATGAA GAAGGTCTGC AGCCCTAACC TAGTCACTTG CAACATAATG 
CTAAAAGCTT ATCTACAAGG CGGATTGTTT GAAGAAGCAA GGGAACTTTT CCAGAAGATG 
TCAGAAGACG GAAATCATAT AAAAAACAGC TCGGATTTCG AATCAAGAGT ATTGCCAGAC 
ACGTACACGT TCAACACGAT GCTAGACACG TGTGCTGAAC AAGAAAAGTG GGATGATTTT 
GGTTATGCGT ATCGGGAGAT GTTGCGTCAT GGATACCATT TCAATGCGAA ACGCCATCTC 
AGAATGGTAC TTGAAGCTAG CAGAGCAGGA AAGGAGGAGG TGATGGAAGC GACATGGGAA 
CACATGAGAC GAAGTAATAG AATTCCGCCA TCGCCTCTAA TCAAAGAAAG ATTCTTCAGG 
AAACTCGAGA AAGGCGATCA TATTTCGGCT ATATCATCTC TTGCTGATCT TAATGGAAAA 
ATTGAGGAGA CTGAGTTACG AGCATTTTCA ACTTCCGCAT GGTCCAGAGT CTTGTCCCGA 
TTTGAGCAAG ATTCAGTTTT GAGGTTAATG GACGATGTGA ACAGACGCCT AGGTTCGAGA 
AGTGAGTCTT CGGATTCGGT TTTGGGGAAT CTATTGAGTT CTTGTAAAGA TTATCTGAAG 
ACCAGAACAC ATAACTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.
Nature408(6814)816-20(2000 Dec)
Theologis A,Ecker JR,Palm CJ,Federspiel NA,Kaul S,White O,Alonso J,Altafi H,Araujo R,Bowman CL,Brooks SY,Buehler E,Chan A,Chao Q,Chen H,Cheuk RF,Chin CW,Chung MK,Conn L,Conway AB,Conway AR,Creasy TH,Dewar K,Dunn P,Etgu P,Feldblyum TV,Feng J,Fong B,Fujii CY,Gill JE,Goldsmith AD,Haas B,Hansen NF,Hughes B,Huizar L,Hunter JL,Jenkins J,Johnson-Hopson C,Khan S,Khaykin E,Kim CJ,Koo HL,Kremenetskaia I,Kurtz DB,Kwan A,Lam B,Langin-Hooper S,Lee A,Lee JM,Lenz CA,Li JH,Li Y,Lin X,Liu SX,Liu ZA,Luros JS,Maiti R,Marziali A,Militscher J,Miranda M,Nguyen M,Nierman WC,Osborne BI,Pai G,Peterson J,Pham PK,Rizzo M,Rooney T,Rowley D,Sakano H,Salzberg SL,Schwartz JR,Shinn P,Southwick AM,Sun H,Tallon LJ,Tambunga G,Toriumi MJ,Town CD,Utterback T,Van Aken S,Vaysberg M,Vysotskaia VS,Walker M,Wu D,Yu G,Fraser CM,Venter JC,Davis RW

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EMB2279 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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