AT4G27220 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	AT4G27220
Entrez Gene ID	828830
Full Name	NB-ARC domain-containing disease resistance protein
Synonyms	M4I22.30,M4I22_30
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118856.1	NP_194452.1	NB-ARC domain-containing disease resistance protein
NM_001341839.1	NP_001328414.1	NB-ARC domain-containing disease resistance protein

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT4G27190	NP_194449.1
Arabidopsis thaliana (thale cress)	AT4G27220	NP_194452.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AT4G27220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AT4G27220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16446	NM_118856.1 Latest version!	Arabidopsis thaliana NB-ARC domain-containing disease resistance protein (AT4G27220), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OAb39777	NM_001341839.1 Latest version!	Arabidopsis thaliana NB-ARC domain-containing disease resistance protein (AT4G27220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AT4G27220 ( NM_118856.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_194452.1 Arabidopsis thaliana NB-ARC domain-containing disease resistance protein (AT4G27220), partial mRNA.

Price & Availability↑

CloneID	OAb16446
Clone ID Related Accession (Same CDS sequence)	NM_118856.1
Accession Version	NM_118856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NB-ARC domain-containing disease resistance protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). COMPLETENESS: incomplete on both ends.

ATGTTCAGAT CGAACGCAAG AGCGTTGAAT AGAGCATTGG AGAGACTAAA GAATGTGCAG 
ACCAAGGTGA ATGAAGCTCT TAAGAGATCA GGGATCCAAG AGAAATCTTT GGAGCGAAAG 
CTGAGGATAT GGCTAAGAAA GGTCGAAGAG AATGTGCCTC TTGGTGAGCT GATTTTGGAG 
AAACGCTCTT CATGTGCTAT ATGGCTGAGC GACAAAGATG TGGAAATTCT TGAGAAAGTC 
AAAAGGCTGG AGGAGCAAGG CCAAGATCTC ATAAAAAAGA TTTCAGTCAA CAAGTCATCT 
AGAGAAATAG TTGAACGGGT CTTGGGGCCT TCATTTCATC CTCAAAAAAC AGCACTGGAG 
ATGTTGGATA AGCTAAAGGA TTGTTTGAAG AAGAAGAATG TTCAAAAGAT TGGTGTTTGG 
GGTATGGGCG GTGTTGGGAA GACAACACTT GTCAGGACGC TTAACAATGA TCTCTTGAAA 
TATGCTGCTA CTCAACAGTT TGCTTTGGTA ATTTGGGTGA CAGTGTCGAA AGATTTTGAC 
TTAAAAAGGG TCCAGATGGA CATAGCCAAG AGACTGGGCA AGCGATTTAC AAGGGAGCAA 
ATGAATCAGC TAGGCTTAAC TATTTGTGAG AGGCTAATAG ATTTGAAAAA TTTCCTCCTT 
ATTCTTGACG ATGTTTGGCA CCCCATTGAT TTGGACCAAT TGGGTATTCC ATTAGCATTG 
GAACGATCGA AGGATTCAAA GGTTGTTCTA ACTTCAAGAA GACTAGAAGT GTGCCAACAG 
ATGATGACTA ATGAAAACAT CAAAGTTGCA TGCTTACAAG AGAAAGAAGC TTGGGAATTG 
TTTTGCCACA ACGTTGGAGA AGTAGCAAAC TCTGATAATG TTAAGCCCAT TGCAAAGGAT 
GTATCCCATG AATGTTGTGG ATTGCCTTTG GCTATCATCA CAATCGGGCG GACCCTGCGC 
GGAAAACCTC AAGTCGAGGT ATGGAAACAC ACCTTGAATT TGCTAAAGAG ATCAGCACCT 
TCTATCGATA CTGAAGAAAA GATCTTTGGT ACTTTAAAAC TCAGCTACGA CTTTCTTCAA 
GACAATATGA AAAGTTGCTT CCTTTTCTGT GCTTTATTTC CGGAGGACTA CTCTATCAAA 
GTAAGTGAAC TAATAATGTA CTGGGTCGCG GAAGGATTGT TGGATGGACA ACATCATTAC 
GAGGACATGA TGAATGAGGG AGTTACTTTG GTTGAGAGAT TGAAGGACTC TTGCCTGTTA 
GAAGATGGTG ATAGTTGTGA TACAGTTAAA ATGCACGATG TTGTGCGTGA CTTTGCTATA 
TGGTTCATGT CTTCTCAGGG TGAAGGATTT CATTCTCTTG TCATGGCCGG GAGAGGTCTA 
ATAGAGTTTC CACAAGATAA GTTTGTTTCT TCAGTCCAGA GAGTTTCTTT GATGGCTAAT 
AAGCTAGAAA GACTACCTAA TAATGTGATA GAGGGTGTGG AAACATTAGT TTTACTACTA 
CAAGGGAATT CCCATGTTAA GGAAGTTCCT AATGGGTTCT TACAAGCATT CCCAAACCTT 
AGAATCTTGG ATCTCAGTGG TGTTCGTATA AGAACATTGC CAGACTCCTT TTCAAATCTT 
CATAGTTTGC GGTCTCTTGT GTTAAGAAAC TGTAAAAAAT TGAGGAACCT GCCTTCGCTA 
GAATCCCTTG TTAAACTCCA ATTTCTTGAT CTACATGAAT CTGCCATTAG GGAACTTCCG 
AGAGGTCTTG AAGCGTTGAG CAGCTTAAGA TACATTTGCG TTTCCAACAC ATATCAACTC 
CAGAGCATTC CGGCTGGAAC TATTCTCCAG TTATCAAGTT TGGAGGTTCT AGACATGGCG 
GGGAGTGCTT ATAGTTGGGG CATCAAGGGA GAAGAAAGAG AAGGACAAGC AACACTTGAC 
GAGGTTACAT GCCTCCCTCA TTTGCAGTTT CTTGCCATTA AGCTCCTCGA TGTCTTGTCC 
TTTTCCTATG AATTTGATTC TTTGACCAAA AGGCTAACAA AGTTCCAGTT TCTATTTTCA 
CCTATTCGAT CGGTTTCACC TCCTGGCACC GGTGAAGGTT GCCTAGCAAT CAGTGATGTC 
AATGTCTCAA ATGCATCTAT TGGGTGGCTA CTGCAACATG TGACATCACT AGACTTGAAC 
TATTGTGAGG GTCTTAATGG TATGTTCGAG AACTTGGTAA CGAAGAGCAA AAGTAGCTTT 
GTTGCCATGA AAGCACTGTC CATTCACTAT TTTCCCAGCT TAAGCCTTGC GAGTGGTTGT 
GAGTCCCAAC TGGATCTCTT TCCAAATCTC GAGGAACTTT CTTTGGACAA TGTCAATCTG 
GAAAGCATCG GAGAACTTAA TGGTTTCCTC GGGATGAGAC TTCAGAAACT AAAACTACTT 
CAAGTCTCTG GCTGCCGACA GCTTAAACGT CTTTTCTCAG ACCAAATCTT AGCAGGTACT 
CTGCCAAACC TACAAGAGAT AAAGGTTGTC TCATGTTTGA GGTTGGAGGA GCTCTTCAAT 
TTTTCTTCAG TGCCAGTTGA TTTTTGTGCT GAGTCTTTGC TACCTAAGCT AACTGTCATA 
AAACTCAAAT ATCTTCCACA ATTAAGGAGC TTATGCAACG ACAGAGTGGT GCTCGAATCC 
CTGGAACACT TAGAAGTCGA ATCTTGTGAA AGTTTAAAGA ACTTACCCTT TGTCCCCGGC 
AATACTGGTA TGATCAACGA ACAAATGGCG TGGGAATACA TGTCTAGGAC TCTAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_194452.1
CDS	1..2760
Translation	MFRSNARALNRALERLKNVQTKVNEALKRSGIQEKSLERKLRIWLRKVEENVPLGELILEKRSSCAIWLSDKDVEILEKVKRLEEQGQDLIKKISVNKSSREIVERVLGPSFHPQKTALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIWVTVSKDFDLKRVQMDIAKRLGKRFTREQMNQLGLTICERLIDLKNFLLILDDVWHPIDLDQLGIPLALERSKDSKVVLTSRRLEVCQQMMTNENIKVACLQEKEAWELFCHNVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKRSAPSIDTEEKIFGTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDTVKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILDLSGVRIRTLPDSFSNLHSLRSLVLRNCKKLRNLPSLESLVKLQFLDLHESAIRELPRGLEALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEEREGQATLDEVTCLPHLQFLAIKLLDVLSFSYEFDSLTKRLTKFQFLFSPIRSVSPPGTGEGCLAISDVNVSNASIGWLLQHVTSLDLNYCEGLNGMFENLVTKSKSSFVAMKALSIHYFPSLSLASGCESQLDLFPNLEELSLDNVNLESIGELNGFLGMRLQKLKLLQVSGCRQLKRLFSDQILAGTLPNLQEIKVVSCLRLEELFNFSSVPVDFCAESLLPKLTVIKLKYLPQLRSLCNDRVVLESLEHLEVESCESLKNLPFVPGNTGMINEQMAWEYMSRTLG

Target ORF information:

RefSeq Version	NM_118856.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NB-ARC domain-containing disease resistance protein (AT4G27220), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118856.1

ORF Insert Sequence:

ATGTTCAGAT CGAACGCAAG AGCGTTGAAT AGAGCATTGG AGAGACTAAA GAATGTGCAG 
ACCAAGGTGA ATGAAGCTCT TAAGAGATCA GGGATCCAAG AGAAATCTTT GGAGCGAAAG 
CTGAGGATAT GGCTAAGAAA GGTCGAAGAG AATGTGCCTC TTGGTGAGCT GATTTTGGAG 
AAACGCTCTT CATGTGCTAT ATGGCTGAGC GACAAAGATG TGGAAATTCT TGAGAAAGTC 
AAAAGGCTGG AGGAGCAAGG CCAAGATCTC ATAAAAAAGA TTTCAGTCAA CAAGTCATCT 
AGAGAAATAG TTGAACGGGT CTTGGGGCCT TCATTTCATC CTCAAAAAAC AGCACTGGAG 
ATGTTGGATA AGCTAAAGGA TTGTTTGAAG AAGAAGAATG TTCAAAAGAT TGGTGTTTGG 
GGTATGGGCG GTGTTGGGAA GACAACACTT GTCAGGACGC TTAACAATGA TCTCTTGAAA 
TATGCTGCTA CTCAACAGTT TGCTTTGGTA ATTTGGGTGA CAGTGTCGAA AGATTTTGAC 
TTAAAAAGGG TCCAGATGGA CATAGCCAAG AGACTGGGCA AGCGATTTAC AAGGGAGCAA 
ATGAATCAGC TAGGCTTAAC TATTTGTGAG AGGCTAATAG ATTTGAAAAA TTTCCTCCTT 
ATTCTTGACG ATGTTTGGCA CCCCATTGAT TTGGACCAAT TGGGTATTCC ATTAGCATTG 
GAACGATCGA AGGATTCAAA GGTTGTTCTA ACTTCAAGAA GACTAGAAGT GTGCCAACAG 
ATGATGACTA ATGAAAACAT CAAAGTTGCA TGCTTACAAG AGAAAGAAGC TTGGGAATTG 
TTTTGCCACA ACGTTGGAGA AGTAGCAAAC TCTGATAATG TTAAGCCCAT TGCAAAGGAT 
GTATCCCATG AATGTTGTGG ATTGCCTTTG GCTATCATCA CAATCGGGCG GACCCTGCGC 
GGAAAACCTC AAGTCGAGGT ATGGAAACAC ACCTTGAATT TGCTAAAGAG ATCAGCACCT 
TCTATCGATA CTGAAGAAAA GATCTTTGGT ACTTTAAAAC TCAGCTACGA CTTTCTTCAA 
GACAATATGA AAAGTTGCTT CCTTTTCTGT GCTTTATTTC CGGAGGACTA CTCTATCAAA 
GTAAGTGAAC TAATAATGTA CTGGGTCGCG GAAGGATTGT TGGATGGACA ACATCATTAC 
GAGGACATGA TGAATGAGGG AGTTACTTTG GTTGAGAGAT TGAAGGACTC TTGCCTGTTA 
GAAGATGGTG ATAGTTGTGA TACAGTTAAA ATGCACGATG TTGTGCGTGA CTTTGCTATA 
TGGTTCATGT CTTCTCAGGG TGAAGGATTT CATTCTCTTG TCATGGCCGG GAGAGGTCTA 
ATAGAGTTTC CACAAGATAA GTTTGTTTCT TCAGTCCAGA GAGTTTCTTT GATGGCTAAT 
AAGCTAGAAA GACTACCTAA TAATGTGATA GAGGGTGTGG AAACATTAGT TTTACTACTA 
CAAGGGAATT CCCATGTTAA GGAAGTTCCT AATGGGTTCT TACAAGCATT CCCAAACCTT 
AGAATCTTGG ATCTCAGTGG TGTTCGTATA AGAACATTGC CAGACTCCTT TTCAAATCTT 
CATAGTTTGC GGTCTCTTGT GTTAAGAAAC TGTAAAAAAT TGAGGAACCT GCCTTCGCTA 
GAATCCCTTG TTAAACTCCA ATTTCTTGAT CTACATGAAT CTGCCATTAG GGAACTTCCG 
AGAGGTCTTG AAGCGTTGAG CAGCTTAAGA TACATTTGCG TTTCCAACAC ATATCAACTC 
CAGAGCATTC CGGCTGGAAC TATTCTCCAG TTATCAAGTT TGGAGGTTCT AGACATGGCG 
GGGAGTGCTT ATAGTTGGGG CATCAAGGGA GAAGAAAGAG AAGGACAAGC AACACTTGAC 
GAGGTTACAT GCCTCCCTCA TTTGCAGTTT CTTGCCATTA AGCTCCTCGA TGTCTTGTCC 
TTTTCCTATG AATTTGATTC TTTGACCAAA AGGCTAACAA AGTTCCAGTT TCTATTTTCA 
CCTATTCGAT CGGTTTCACC TCCTGGCACC GGTGAAGGTT GCCTAGCAAT CAGTGATGTC 
AATGTCTCAA ATGCATCTAT TGGGTGGCTA CTGCAACATG TGACATCACT AGACTTGAAC 
TATTGTGAGG GTCTTAATGG TATGTTCGAG AACTTGGTAA CGAAGAGCAA AAGTAGCTTT 
GTTGCCATGA AAGCACTGTC CATTCACTAT TTTCCCAGCT TAAGCCTTGC GAGTGGTTGT 
GAGTCCCAAC TGGATCTCTT TCCAAATCTC GAGGAACTTT CTTTGGACAA TGTCAATCTG 
GAAAGCATCG GAGAACTTAA TGGTTTCCTC GGGATGAGAC TTCAGAAACT AAAACTACTT 
CAAGTCTCTG GCTGCCGACA GCTTAAACGT CTTTTCTCAG ACCAAATCTT AGCAGGTACT 
CTGCCAAACC TACAAGAGAT AAAGGTTGTC TCATGTTTGA GGTTGGAGGA GCTCTTCAAT 
TTTTCTTCAG TGCCAGTTGA TTTTTGTGCT GAGTCTTTGC TACCTAAGCT AACTGTCATA 
AAACTCAAAT ATCTTCCACA ATTAAGGAGC TTATGCAACG ACAGAGTGGT GCTCGAATCC 
CTGGAACACT TAGAAGTCGA ATCTTGTGAA AGTTTAAAGA ACTTACCCTT TGTCCCCGGC 
AATACTGGTA TGATCAACGA ACAAATGGCG TGGGAATACA TGTCTAGGAC TCTAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AT4G27220 ( NM_001341839.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328414.1 Arabidopsis thaliana NB-ARC domain-containing disease resistance protein (AT4G27220), mRNA.

Price & Availability↑

CloneID	OAb39777
Clone ID Related Accession (Same CDS sequence)	NM_001341839.1
Accession Version	NM_001341839.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NB-ARC domain-containing disease resistance protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGTGTTCGA GAGCCCATAG AGTCATGTTC AGATCGAACG CAAGAGCGTT GAATAGAGCA 
TTGGAGAGAC TAAAGAATGT GCAGACCAAG GTGAATGAAG CTCTTAAGAG ATCAGGGATC 
CAAGAGAAAT CTTTGGAGCG AAAGCTGAGG ATATGGCTAA GAAAGGTCGA AGAGAATGTG 
CCTCTTGGTG AGCTGATTTT GGAGAAACGC TCTTCATGTG CTATATGGCT GAGCGACAAA 
GATGTGGAAA TTCTTGAGAA AGTCAAAAGG CTGGAGGAGC AAGGCCAAGA TCTCATAAAA 
AAGATTTCAG TCAACAAGTC ATCTAGAGAA ATAGTTGAAC GGGTCTTGGG GCCTTCATTT 
CATCCTCAAA AAACAGCACT GGAGATGTTG GATAAGCTAA AGGATTGTTT GAAGAAGAAG 
AATGTTCAAA AGATTGGTGT TTGGGGTATG GGCGGTGTTG GGAAGACAAC ACTTGTCAGG 
ACGCTTAACA ATGATCTCTT GAAATATGCT GCTACTCAAC AGTTTGCTTT GGTAATTTGG 
GTGACAGTGT CGAAAGATTT TGACTTAAAA AGGGTCCAGA TGGACATAGC CAAGAGACTG 
GGCAAGCGAT TTACAAGGGA GCAAATGAAT CAGCTAGGCT TAACTATTTG TGAGAGGCTA 
ATAGATTTGA AAAATTTCCT CCTTATTCTT GACGATGTTT GGCACCCCAT TGATTTGGAC 
CAATTGGGTA TTCCATTAGC ATTGGAACGA TCGAAGGATT CAAAGGTTGT TCTAACTTCA 
AGAAGACTAG AAGTGTGCCA ACAGATGATG ACTAATGAAA ACATCAAAGT TGCATGCTTA 
CAAGAGAAAG AAGCTTGGGA ATTGTTTTGC CACAACGTTG GAGAAGTAGC AAACTCTGAT 
AATGTTAAGC CCATTGCAAA GGATGTATCC CATGAATGTT GTGGATTGCC TTTGGCTATC 
ATCACAATCG GGCGGACCCT GCGCGGAAAA CCTCAAGTCG AGGTATGGAA ACACACCTTG 
AATTTGCTAA AGAGATCAGC ACCTTCTATC GATACTGAAG AAAAGATCTT TGGTACTTTA 
AAACTCAGCT ACGACTTTCT TCAAGACAAT ATGAAAAGTT GCTTCCTTTT CTGTGCTTTA 
TTTCCGGAGG ACTACTCTAT CAAAGTAAGT GAACTAATAA TGTACTGGGT CGCGGAAGGA 
TTGTTGGATG GACAACATCA TTACGAGGAC ATGATGAATG AGGGAGTTAC TTTGGTTGAG 
AGATTGAAGG ACTCTTGCCT GTTAGAAGAT GGTGATAGTT GTGATACAGT TAAAATGCAC 
GATGTTGTGC GTGACTTTGC TATATGGTTC ATGTCTTCTC AGGGTGAAGG ATTTCATTCT 
CTTGTCATGG CCGGGAGAGG TCTAATAGAG TTTCCACAAG ATAAGTTTGT TTCTTCAGTC 
CAGAGAGTTT CTTTGATGGC TAATAAGCTA GAAAGACTAC CTAATAATGT GATAGAGGGT 
GTGGAAACAT TAGTTTTACT ACTACAAGGG AATTCCCATG TTAAGGAAGT TCCTAATGGG 
TTCTTACAAG CATTCCCAAA CCTTAGAATC TTGGATCTCA GTGGTGTTCG TATAAGAACA 
TTGCCAGACT CCTTTTCAAA TCTTCATAGT TTGCGGTCTC TTGTGTTAAG AAACTGTAAA 
AAATTGAGGA ACCTGCCTTC GCTAGAATCC CTTGTTAAAC TCCAATTTCT TGATCTACAT 
GAATCTGCCA TTAGGGAACT TCCGAGAGGT CTTGAAGCGT TGAGCAGCTT AAGATACATT 
TGCGTTTCCA ACACATATCA ACTCCAGAGC ATTCCGGCTG GAACTATTCT CCAGTTATCA 
AGTTTGGAGG TTCTAGACAT GGCGGGGAGT GCTTATAGTT GGGGCATCAA GGGAGAAGAA 
AGAGAAGGAC AAGCAACACT TGACGAGGTT ACATGCCTCC CTCATTTGCA GTTTCTTGCC 
ATTAAGCTCC TCGATGTCTT GTCCTTTTCC TATGAATTTG ATTCTTTGAC CAAAAGGCTA 
ACAAAGTTCC AGTTTCTATT TTCACCTATT CGATCGGTTT CACCTCCTGG CACCGGTGAA 
GGTTGCCTAG CAATCAGTGA TGTCAATGTC TCAAATGCAT CTATTGGGTG GCTACTGCAA 
CATGTGACAT CACTAGACTT GAACTATTGT GAGGGTCTTA ATGGTATGTT CGAGAACTTG 
GTAACGAAGA GCAAAAGTAG CTTTGTTGCC ATGAAAGCAC TGTCCATTCA CTATTTTCCC 
AGCTTAAGCC TTGCGAGTGG TTGTGAGTCC CAACTGGATC TCTTTCCAAA TCTCGAGGAA 
CTTTCTTTGG ACAATGTCAA TCTGGAAAGC ATCGGAGAAC TTAATGGTTT CCTCGGGATG 
AGACTTCAGA AACTAAAACT ACTTCAAGTC TCTGGCTGCC GACAGCTTAA ACGTCTTTTC 
TCAGACCAAA TCTTAGCAGG TACTCTGCCA AACCTACAAG AGATAAAGGT TGTCTCATGT 
TTGAGGTTGG AGGAGCTCTT CAATTTTTCT TCAGTGCCAG TTGATTTTTG TGCTGAGTCT 
TTGCTACCTA AGCTAACTGT CATAAAACTC AAATATCTTC CACAATTAAG GAGCTTATGC 
AACGACAGAG TGGTGCTCGA ATCCCTGGAA CACTTAGAAG TCGAATCTTG TGAAAGTTTA 
AAGAACTTAC CCTTTGTCCC CGGCAATACT GGTATGATCA ACGAACAAAT GGCGTGGGAA 
TACATGTCTA GGACTCTAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328414.1
CDS	384..3167
Translation	MCSRAHRVMFRSNARALNRALERLKNVQTKVNEALKRSGIQEKSLERKLRIWLRKVEENVPLGELILEKRSSCAIWLSDKDVEILEKVKRLEEQGQDLIKKISVNKSSREIVERVLGPSFHPQKTALEMLDKLKDCLKKKNVQKIGVWGMGGVGKTTLVRTLNNDLLKYAATQQFALVIWVTVSKDFDLKRVQMDIAKRLGKRFTREQMNQLGLTICERLIDLKNFLLILDDVWHPIDLDQLGIPLALERSKDSKVVLTSRRLEVCQQMMTNENIKVACLQEKEAWELFCHNVGEVANSDNVKPIAKDVSHECCGLPLAIITIGRTLRGKPQVEVWKHTLNLLKRSAPSIDTEEKIFGTLKLSYDFLQDNMKSCFLFCALFPEDYSIKVSELIMYWVAEGLLDGQHHYEDMMNEGVTLVERLKDSCLLEDGDSCDTVKMHDVVRDFAIWFMSSQGEGFHSLVMAGRGLIEFPQDKFVSSVQRVSLMANKLERLPNNVIEGVETLVLLLQGNSHVKEVPNGFLQAFPNLRILDLSGVRIRTLPDSFSNLHSLRSLVLRNCKKLRNLPSLESLVKLQFLDLHESAIRELPRGLEALSSLRYICVSNTYQLQSIPAGTILQLSSLEVLDMAGSAYSWGIKGEEREGQATLDEVTCLPHLQFLAIKLLDVLSFSYEFDSLTKRLTKFQFLFSPIRSVSPPGTGEGCLAISDVNVSNASIGWLLQHVTSLDLNYCEGLNGMFENLVTKSKSSFVAMKALSIHYFPSLSLASGCESQLDLFPNLEELSLDNVNLESIGELNGFLGMRLQKLKLLQVSGCRQLKRLFSDQILAGTLPNLQEIKVVSCLRLEELFNFSSVPVDFCAESLLPKLTVIKLKYLPQLRSLCNDRVVLESLEHLEVESCESLKNLPFVPGNTGMINEQMAWEYMSRTLG

Target ORF information:

RefSeq Version	NM_001341839.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NB-ARC domain-containing disease resistance protein (AT4G27220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341839.1

ORF Insert Sequence:

ATGTGTTCGA GAGCCCATAG AGTCATGTTC AGATCGAACG CAAGAGCGTT GAATAGAGCA 
TTGGAGAGAC TAAAGAATGT GCAGACCAAG GTGAATGAAG CTCTTAAGAG ATCAGGGATC 
CAAGAGAAAT CTTTGGAGCG AAAGCTGAGG ATATGGCTAA GAAAGGTCGA AGAGAATGTG 
CCTCTTGGTG AGCTGATTTT GGAGAAACGC TCTTCATGTG CTATATGGCT GAGCGACAAA 
GATGTGGAAA TTCTTGAGAA AGTCAAAAGG CTGGAGGAGC AAGGCCAAGA TCTCATAAAA 
AAGATTTCAG TCAACAAGTC ATCTAGAGAA ATAGTTGAAC GGGTCTTGGG GCCTTCATTT 
CATCCTCAAA AAACAGCACT GGAGATGTTG GATAAGCTAA AGGATTGTTT GAAGAAGAAG 
AATGTTCAAA AGATTGGTGT TTGGGGTATG GGCGGTGTTG GGAAGACAAC ACTTGTCAGG 
ACGCTTAACA ATGATCTCTT GAAATATGCT GCTACTCAAC AGTTTGCTTT GGTAATTTGG 
GTGACAGTGT CGAAAGATTT TGACTTAAAA AGGGTCCAGA TGGACATAGC CAAGAGACTG 
GGCAAGCGAT TTACAAGGGA GCAAATGAAT CAGCTAGGCT TAACTATTTG TGAGAGGCTA 
ATAGATTTGA AAAATTTCCT CCTTATTCTT GACGATGTTT GGCACCCCAT TGATTTGGAC 
CAATTGGGTA TTCCATTAGC ATTGGAACGA TCGAAGGATT CAAAGGTTGT TCTAACTTCA 
AGAAGACTAG AAGTGTGCCA ACAGATGATG ACTAATGAAA ACATCAAAGT TGCATGCTTA 
CAAGAGAAAG AAGCTTGGGA ATTGTTTTGC CACAACGTTG GAGAAGTAGC AAACTCTGAT 
AATGTTAAGC CCATTGCAAA GGATGTATCC CATGAATGTT GTGGATTGCC TTTGGCTATC 
ATCACAATCG GGCGGACCCT GCGCGGAAAA CCTCAAGTCG AGGTATGGAA ACACACCTTG 
AATTTGCTAA AGAGATCAGC ACCTTCTATC GATACTGAAG AAAAGATCTT TGGTACTTTA 
AAACTCAGCT ACGACTTTCT TCAAGACAAT ATGAAAAGTT GCTTCCTTTT CTGTGCTTTA 
TTTCCGGAGG ACTACTCTAT CAAAGTAAGT GAACTAATAA TGTACTGGGT CGCGGAAGGA 
TTGTTGGATG GACAACATCA TTACGAGGAC ATGATGAATG AGGGAGTTAC TTTGGTTGAG 
AGATTGAAGG ACTCTTGCCT GTTAGAAGAT GGTGATAGTT GTGATACAGT TAAAATGCAC 
GATGTTGTGC GTGACTTTGC TATATGGTTC ATGTCTTCTC AGGGTGAAGG ATTTCATTCT 
CTTGTCATGG CCGGGAGAGG TCTAATAGAG TTTCCACAAG ATAAGTTTGT TTCTTCAGTC 
CAGAGAGTTT CTTTGATGGC TAATAAGCTA GAAAGACTAC CTAATAATGT GATAGAGGGT 
GTGGAAACAT TAGTTTTACT ACTACAAGGG AATTCCCATG TTAAGGAAGT TCCTAATGGG 
TTCTTACAAG CATTCCCAAA CCTTAGAATC TTGGATCTCA GTGGTGTTCG TATAAGAACA 
TTGCCAGACT CCTTTTCAAA TCTTCATAGT TTGCGGTCTC TTGTGTTAAG AAACTGTAAA 
AAATTGAGGA ACCTGCCTTC GCTAGAATCC CTTGTTAAAC TCCAATTTCT TGATCTACAT 
GAATCTGCCA TTAGGGAACT TCCGAGAGGT CTTGAAGCGT TGAGCAGCTT AAGATACATT 
TGCGTTTCCA ACACATATCA ACTCCAGAGC ATTCCGGCTG GAACTATTCT CCAGTTATCA 
AGTTTGGAGG TTCTAGACAT GGCGGGGAGT GCTTATAGTT GGGGCATCAA GGGAGAAGAA 
AGAGAAGGAC AAGCAACACT TGACGAGGTT ACATGCCTCC CTCATTTGCA GTTTCTTGCC 
ATTAAGCTCC TCGATGTCTT GTCCTTTTCC TATGAATTTG ATTCTTTGAC CAAAAGGCTA 
ACAAAGTTCC AGTTTCTATT TTCACCTATT CGATCGGTTT CACCTCCTGG CACCGGTGAA 
GGTTGCCTAG CAATCAGTGA TGTCAATGTC TCAAATGCAT CTATTGGGTG GCTACTGCAA 
CATGTGACAT CACTAGACTT GAACTATTGT GAGGGTCTTA ATGGTATGTT CGAGAACTTG 
GTAACGAAGA GCAAAAGTAG CTTTGTTGCC ATGAAAGCAC TGTCCATTCA CTATTTTCCC 
AGCTTAAGCC TTGCGAGTGG TTGTGAGTCC CAACTGGATC TCTTTCCAAA TCTCGAGGAA 
CTTTCTTTGG ACAATGTCAA TCTGGAAAGC ATCGGAGAAC TTAATGGTTT CCTCGGGATG 
AGACTTCAGA AACTAAAACT ACTTCAAGTC TCTGGCTGCC GACAGCTTAA ACGTCTTTTC 
TCAGACCAAA TCTTAGCAGG TACTCTGCCA AACCTACAAG AGATAAAGGT TGTCTCATGT 
TTGAGGTTGG AGGAGCTCTT CAATTTTTCT TCAGTGCCAG TTGATTTTTG TGCTGAGTCT 
TTGCTACCTA AGCTAACTGT CATAAAACTC AAATATCTTC CACAATTAAG GAGCTTATGC 
AACGACAGAG TGGTGCTCGA ATCCCTGGAA CACTTAGAAG TCGAATCTTG TGAAAGTTTA 
AAGAACTTAC CCTTTGTCCC CGGCAATACT GGTATGATCA ACGAACAAAT GGCGTGGGAA 
TACATGTCTA GGACTCTAGG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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AT4G27220 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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