KT2/3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	KT2/3
Entrez Gene ID	828311
Full Name	potassium transport 2/3
Synonyms	AKT2,AKT2/3,AKT3,T10I14.30,T10I14_30,potassium transport 2/3
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118342.3	NP_567651.1	potassium transport 2/3
NM_001341531.1	NP_001328922.1	potassium transport 2/3

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os05g0428700	NP_001055614.1
Arabidopsis thaliana (thale cress)	KT2/3	NP_567651.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AKT2 subunit containing K(+) channels mediate sodium chloride-induced depolarization of root cells.
Title: The AKT2 potassium channel mediates NaCl induced depolarization in the root of Arabidopsis thaliana.

CBL4 together with the interacting protein kinase CIPK6 modulates the activity and plasma membrane targeting of the K(+) channel AKT2.
Title: Calcium-dependent modulation and plasma membrane targeting of the AKT2 potassium channel by the CBL4/CIPK6 calcium sensor/protein kinase complex.

Data discuss the existence of multiple post-translational modification steps that work in concert to convert AKT2 from an inward-rectifying into a non-rectifying K (+) channel.
Title: The K (+) battery-regulating Arabidopsis K (+) channel AKT2 is under the control of multiple post-translational steps.

AKT2-transformed tobacco mesophyll cells displayed inward-rectifying, rather than voltage-independent K+ channels.
Title: In planta AKT2 subunits constitute a pH- and Ca2+-sensitive inward rectifying K+ channel.

The role of 2 phosphorylation sites on the switch between gating modes of AKT2 are described.
Title: Inward rectification of the AKT2 channel abolished by voltage-dependent phosphorylation.

The following KT2/3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KT2/3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15941	NM_118342.3 Latest version!	Arabidopsis thaliana potassium transport 2/3 (KT2/3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OAb39587	NM_001341531.1 Latest version!	Arabidopsis thaliana potassium transport 2/3 (KT2/3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KT2/3 ( NM_118342.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_567651.1 Arabidopsis thaliana potassium transport 2/3 (KT2/3), mRNA.

Price & Availability↑

CloneID	OAb15941
Clone ID Related Accession (Same CDS sequence)	NM_118342.3
Accession Version	NM_118342.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2409bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	potassium transport 2/3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118342.2.

ATGGACCTCA AGTATTCAGC ATCTCATTGC AACTTATCCT CAGACATGAA GCTCAGGCGT 
TTTCATCAGC ATCGAGGAAA AGGAAGAGAA GAAGAGTATG ATGCTTCTTC TCTCAGCTTG 
AACAATCTGT CAAAACTTAT TCTTCCTCCA CTTGGTGTTG CTAGCTATAA CCAGAATCAC 
ATCAGGTCTA GTGGATGGAT CATCTCACCT ATGGACTCAA GATACAGGTG CTGGGAATTT 
TATATGGTGC TTTTAGTGGC ATACTCTGCG TGGGTTTACC CTTTTGAAGT TGCATTTCTG 
AATTCATCAC CAAAGAGAAA CCTTTGTATC GCGGACAACA TCGTAGACTT GTTCTTCGCG 
GTTGACATTG TCTTGACGTT TTTCGTTGCT TACATAGACG AAAGAACACA GCTTCTTGTC 
CGTGAACCTA AACAGATTGC AGTGAGGTAC CTATCAACAT GGTTCTTGAT GGATGTTGCA 
TCAACTATAC CATTTGACGC TATTGGATAC TTAATCACTG GCACATCCAC GTTAAATATC 
ACTTGTAATC TCTTGGGATT ACTTAGATTT TGGCGACTTC GAAGAGTTAA ACACCTCTTC 
ACTAGGCTCG AGAAGGACAT AAGATATAGC TATTTCTGGA TTCGCTGCTT TCGACTTCTA 
TCAGTGACAT TGTTTCTAGT GCACTGTGCT GGATGCAGTT ATTACCTAAT AGCAGACAGA 
TATCCACACC AAGGAAAGAC ATGGACTGAT GCGATCCCTA ATTTCACAGA GACAAGTCTT 
TCCATCAGAT ACATTGCAGC TATATATTGG TCTATCACTA CAATGACCAC AGTGGGATAT 
GGAGATCTTC ATGCAAGCAA CACTATTGAA ATGGTATTCA TAACAGTCTA CATGTTATTC 
AATCTTGGCC TCACTGCTTA CCTTATTGGT AACATGACTA ATTTGGTCGT GGAAGGGACT 
CGTCGTACCA TGGAATTTAG GAATAGCATT GAAGCAGCGT CAAACTTTGT TAACAGAAAC 
AGATTGCCTC CTAGATTAAA AGACCAGATA TTAGCTTACA TGTGTTTAAG GTTTAAAGCA 
GAGAGCTTAA ATCAGCAACA TCTTATTGAC CAGCTCCCAA AATCTATCTA CAAAAGCATT 
TGTCAACATC TTTTTCTTCC ATCTGTTGAA AAAGTTTACC TCTTCAAAGG CGTCTCAAGA 
GAAATACTTC TTCTTCTGGT TTCAAAAATG AAGGCTGAGT ATATACCACC AAGAGAGGAT 
GTCATTATGC AGAACGAAGC GCCGGATGAT GTTTACATAA TTGTGTCAGG AGAAGTTGAG 
ATCATTGATT CAGAGATGGA GAGAGAGTCT GTTTTAGGCA CTCTACGTTG TGGAGACATA 
TTTGGAGAAG TTGGAGCACT TTGTTGCAGA CCACAAAGCT ACACTTTTCA AACTAAGTCT 
TTATCACAGC TTCTCCGACT CAAAACATCT TTCCTTATTG AGACAATGCA GATTAAACAA 
CAAGACAATG CCACAATGCT CAAGAACTTC TTGCAGCATC ACAAAAAGCT GAGTAATTTA 
GACATTGGTG ATCTAAAGGC ACAACAAAAT GGCGAAAACA CCGATGTTGT TCCTCCTAAC 
ATTGCCTCAA ATCTCATCGC TGTGGTGACT ACAGGCAATG CAGCTCTTCT TGATGAGCTA 
CTTAAGGCTA AGTTAAGCCC TGACATTACA GATTCCAAAG GAAAAACTCC ATTGCATGTA 
GCAGCTTCTA GAGGATATGA AGATTGTGTT TTAGTACTCT TAAAGCACGG TTGCAACATC 
CACATAAGAG ATGTGAATGG TAATAGTGCT CTATGGGAAG CAATAATATC GAAGCATTAC 
GAGATATTCA GAATCCTTTA TCATTTCGCA GCCATATCGG ATCCACACAT AGCTGGAGAT 
CTTCTATGTG AAGCAGCGAA ACAGAACAAT GTAGAAGTCA TGAAGGCTCT TTTAAAACAG 
GGGCTTAACG TCGACACAGA GGATCACCAT GGCGTCACAG CTTTACAGGT CGCTATGGCG 
GAGGATCAGA TGGACATGGT GAATCTCCTG GCGACGAACG GTGCAGATGT AGTTTGTGTG 
AATACACATA ATGAATTCAC ACCATTGGAG AAGTTAAGAG TTGTGGAAGA AGAAGAAGAA 
GAAGAACGAG GAAGAGTGAG TATTTACAGA GGACATCCAT TGGAGAGGAG AGAAAGAAGT 
TGCAATGAAG CTGGGAAGCT TATTCTTCTT CCTCCTTCAC TTGATGACCT CAAGAAAATT 
GCAGGAGAGA AGTTTGGGTT TGATGGAAGT GAGACGATGG TGACGAATGA AGATGGAGCT 
GAGATTGACA GTATTGAAGT GATTAGAGAT AATGACAAAC TCTACTTTGT CGTAAACAAG 
ATAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_567651.1
CDS	608..3016
Translation	MDLKYSASHCNLSSDMKLRRFHQHRGKGREEEYDASSLSLNNLSKLILPPLGVASYNQNHIRSSGWIISPMDSRYRCWEFYMVLLVAYSAWVYPFEVAFLNSSPKRNLCIADNIVDLFFAVDIVLTFFVAYIDERTQLLVREPKQIAVRYLSTWFLMDVASTIPFDAIGYLITGTSTLNITCNLLGLLRFWRLRRVKHLFTRLEKDIRYSYFWIRCFRLLSVTLFLVHCAGCSYYLIADRYPHQGKTWTDAIPNFTETSLSIRYIAAIYWSITTMTTVGYGDLHASNTIEMVFITVYMLFNLGLTAYLIGNMTNLVVEGTRRTMEFRNSIEAASNFVNRNRLPPRLKDQILAYMCLRFKAESLNQQHLIDQLPKSIYKSICQHLFLPSVEKVYLFKGVSREILLLLVSKMKAEYIPPREDVIMQNEAPDDVYIIVSGEVEIIDSEMERESVLGTLRCGDIFGEVGALCCRPQSYTFQTKSLSQLLRLKTSFLIETMQIKQQDNATMLKNFLQHHKKLSNLDIGDLKAQQNGENTDVVPPNIASNLIAVVTTGNAALLDELLKAKLSPDITDSKGKTPLHVAASRGYEDCVLVLLKHGCNIHIRDVNGNSALWEAIISKHYEIFRILYHFAAISDPHIAGDLLCEAAKQNNVEVMKALLKQGLNVDTEDHHGVTALQVAMAEDQMDMVNLLATNGADVVCVNTHNEFTPLEKLRVVEEEEEEERGRVSIYRGHPLERRERSCNEAGKLILLPPSLDDLKKIAGEKFGFDGSETMVTNEDGAEIDSIEVIRDNDKLYFVVNKII

Target ORF information:

RefSeq Version	NM_118342.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana potassium transport 2/3 (KT2/3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118342.3

ORF Insert Sequence:

ATGGACCTCA AGTATTCAGC ATCTCATTGC AACTTATCCT CAGACATGAA GCTCAGGCGT 
TTTCATCAGC ATCGAGGAAA AGGAAGAGAA GAAGAGTATG ATGCTTCTTC TCTCAGCTTG 
AACAATCTGT CAAAACTTAT TCTTCCTCCA CTTGGTGTTG CTAGCTATAA CCAGAATCAC 
ATCAGGTCTA GTGGATGGAT CATCTCACCT ATGGACTCAA GATACAGGTG CTGGGAATTT 
TATATGGTGC TTTTAGTGGC ATACTCTGCG TGGGTTTACC CTTTTGAAGT TGCATTTCTG 
AATTCATCAC CAAAGAGAAA CCTTTGTATC GCGGACAACA TCGTAGACTT GTTCTTCGCG 
GTTGACATTG TCTTGACGTT TTTCGTTGCT TACATAGACG AAAGAACACA GCTTCTTGTC 
CGTGAACCTA AACAGATTGC AGTGAGGTAC CTATCAACAT GGTTCTTGAT GGATGTTGCA 
TCAACTATAC CATTTGACGC TATTGGATAC TTAATCACTG GCACATCCAC GTTAAATATC 
ACTTGTAATC TCTTGGGATT ACTTAGATTT TGGCGACTTC GAAGAGTTAA ACACCTCTTC 
ACTAGGCTCG AGAAGGACAT AAGATATAGC TATTTCTGGA TTCGCTGCTT TCGACTTCTA 
TCAGTGACAT TGTTTCTAGT GCACTGTGCT GGATGCAGTT ATTACCTAAT AGCAGACAGA 
TATCCACACC AAGGAAAGAC ATGGACTGAT GCGATCCCTA ATTTCACAGA GACAAGTCTT 
TCCATCAGAT ACATTGCAGC TATATATTGG TCTATCACTA CAATGACCAC AGTGGGATAT 
GGAGATCTTC ATGCAAGCAA CACTATTGAA ATGGTATTCA TAACAGTCTA CATGTTATTC 
AATCTTGGCC TCACTGCTTA CCTTATTGGT AACATGACTA ATTTGGTCGT GGAAGGGACT 
CGTCGTACCA TGGAATTTAG GAATAGCATT GAAGCAGCGT CAAACTTTGT TAACAGAAAC 
AGATTGCCTC CTAGATTAAA AGACCAGATA TTAGCTTACA TGTGTTTAAG GTTTAAAGCA 
GAGAGCTTAA ATCAGCAACA TCTTATTGAC CAGCTCCCAA AATCTATCTA CAAAAGCATT 
TGTCAACATC TTTTTCTTCC ATCTGTTGAA AAAGTTTACC TCTTCAAAGG CGTCTCAAGA 
GAAATACTTC TTCTTCTGGT TTCAAAAATG AAGGCTGAGT ATATACCACC AAGAGAGGAT 
GTCATTATGC AGAACGAAGC GCCGGATGAT GTTTACATAA TTGTGTCAGG AGAAGTTGAG 
ATCATTGATT CAGAGATGGA GAGAGAGTCT GTTTTAGGCA CTCTACGTTG TGGAGACATA 
TTTGGAGAAG TTGGAGCACT TTGTTGCAGA CCACAAAGCT ACACTTTTCA AACTAAGTCT 
TTATCACAGC TTCTCCGACT CAAAACATCT TTCCTTATTG AGACAATGCA GATTAAACAA 
CAAGACAATG CCACAATGCT CAAGAACTTC TTGCAGCATC ACAAAAAGCT GAGTAATTTA 
GACATTGGTG ATCTAAAGGC ACAACAAAAT GGCGAAAACA CCGATGTTGT TCCTCCTAAC 
ATTGCCTCAA ATCTCATCGC TGTGGTGACT ACAGGCAATG CAGCTCTTCT TGATGAGCTA 
CTTAAGGCTA AGTTAAGCCC TGACATTACA GATTCCAAAG GAAAAACTCC ATTGCATGTA 
GCAGCTTCTA GAGGATATGA AGATTGTGTT TTAGTACTCT TAAAGCACGG TTGCAACATC 
CACATAAGAG ATGTGAATGG TAATAGTGCT CTATGGGAAG CAATAATATC GAAGCATTAC 
GAGATATTCA GAATCCTTTA TCATTTCGCA GCCATATCGG ATCCACACAT AGCTGGAGAT 
CTTCTATGTG AAGCAGCGAA ACAGAACAAT GTAGAAGTCA TGAAGGCTCT TTTAAAACAG 
GGGCTTAACG TCGACACAGA GGATCACCAT GGCGTCACAG CTTTACAGGT CGCTATGGCG 
GAGGATCAGA TGGACATGGT GAATCTCCTG GCGACGAACG GTGCAGATGT AGTTTGTGTG 
AATACACATA ATGAATTCAC ACCATTGGAG AAGTTAAGAG TTGTGGAAGA AGAAGAAGAA 
GAAGAACGAG GAAGAGTGAG TATTTACAGA GGACATCCAT TGGAGAGGAG AGAAAGAAGT 
TGCAATGAAG CTGGGAAGCT TATTCTTCTT CCTCCTTCAC TTGATGACCT CAAGAAAATT 
GCAGGAGAGA AGTTTGGGTT TGATGGAAGT GAGACGATGG TGACGAATGA AGATGGAGCT 
GAGATTGACA GTATTGAAGT GATTAGAGAT AATGACAAAC TCTACTTTGT CGTAAACAAG 
ATAATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KT2/3 ( NM_001341531.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001328922.1 Arabidopsis thaliana potassium transport 2/3 (KT2/3), mRNA.

Price & Availability↑

CloneID	OAb39587
Clone ID Related Accession (Same CDS sequence)	NM_001341531.1
Accession Version	NM_001341531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	potassium transport 2/3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGACCTCA AGTATTCAGC ATCTCATTGC AACTTATCCT CAGACATGAA GCTCAGGCGT 
TTTCATCAGC ATCGAGGAAA AGGAAGAGAA GAAGAGTATG ATGCTTCTTC TCTCAGCTTG 
AACAATCTGT CAAAACTTAT TCTTCCTCCA CTTGGTGTTG CTAGCTATAA CCAGAATCAC 
ATCAGGTCTA GTGGATGGAT CATCTCACCT ATGGACTCAA GATACAGGTG CTGGGAATTT 
TATATGGTGC TTTTAGTGGC ATACTCTGCG TGGGTTTACC CTTTTGAAGT TGCATTTCTG 
AATTCATCAC CAAAGAGAAA CCTTTGTATC GCGGACAACA TCGTAGACTT GTTCTTCGCG 
GTTGACATTG TCTTGACGTT TTTCGTTGCT TACATAGACG AAAGAACACA GCTTCTTGTC 
CGTGAACCTA AACAGATTGC AGTGAGGTAC CTATCAACAT GGTTCTTGAT GGATGTTGCA 
TCAACTATAC CATTTGACGC TATTGGATAC TTAATCACTG GCACATCCAC GTTAAATATC 
ACTTGTAATC TCTTGGGATT ACTTAGATTT TGGCGACTTC GAAGAGTTAA ACACCTCTTC 
ACTAGGCTCG AGAAGGACAT AAGATATAGC TATTTCTGGA TTCGCTGCTT TCGACTTCTA 
TCAGTGACAT TGTTTCTAGT GCACTGTGCT GGATGCAGTT ATTACCTAAT AGCAGACAGA 
TATCCACACC AAGGAAAGAC ATGGACTGAT GCGATCCCTA ATTTCACAGA GACAAGTCTT 
TCCATCAGAT ACATTGCAGC TATATATTGG TCTATCACTA CAATGACCAC AGTGGGATAT 
GGAGATCTTC ATGCAAGCAA CACTATTGAA ATGGTATTCA TAACAGTCTA CATGTTATTC 
AATCTTGGCC TCACTGCTTA CCTTATTGGT AACATGACTA ATTTGGTCGT GGAAGGGACT 
CGTCGTACCA TGGAATTTAG GAATAGCATT GAAGCAGCGT CAAACTTTGT TAACAGAAAC 
AGATTGCCTC CTAGATTAAA AGACCAGATA TTAGCTTACA TGTGTTTAAG GTTTAAAGCA 
GAGAGCTTAA ATCAGCAACA TCTTATTGAC CAGCTCCCAA AATCTATCTA CAAAAGCATT 
TGTCAACATC TTTTTCTTCC ATCTGTTGAA AAAGTTTACC TCTTCAAAGG CGTCTCAAGA 
GAAATACTTC TTCTTCTGGT TTCAAAAATG AAGGCTGAGT ATATACCACC AAGAGAGGAT 
GTCATTATGC AGAACGAAGC GCCGGATGAT GTTTACATAA TTGTGTCAGG AGAAGTTGAG 
ATCATTGATT CAGAGATGGA GAGAGAGTCT GTTTTAGGCA CTCTACGTTG TGGAGACATA 
TTTGGAGAAG TTGGAGCACT TTGTTGCAGA CCACAAAGCT ACACTTTTCA AACTAAGTCT 
TTATCACAGC TTCTCCGACT CAAAACATCT TTCCTTATTG AGACAATGCA GATTAAACAA 
CAAGACAATG CCACAATGCT CAAGAACTTC TTGCAGCATC ACAAAAAGCT GAGTAATTTA 
GACATTGGTG ATCTAAAGGC ACAACAAAAT GGCGAAAACA CCGATGTTGT TCCTCCTAAC 
ATTGCCTCAA ATCTCATCGC TGTGGTGACT ACAGGCAATG CAGCTCTTCT TGATGAGCTA 
CTTAAGGCTA AGTTAAGCCC TGACATTACA GATTCCAAAG GAAAAACTCC ATTGGTAACG 
TTTTGCTTTC ACCATAAGCC ATTTAAAACC CTTACTCATA ATCATCATCA CAGCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001328922.1
CDS	608..2407
Translation	MDLKYSASHCNLSSDMKLRRFHQHRGKGREEEYDASSLSLNNLSKLILPPLGVASYNQNHIRSSGWIISPMDSRYRCWEFYMVLLVAYSAWVYPFEVAFLNSSPKRNLCIADNIVDLFFAVDIVLTFFVAYIDERTQLLVREPKQIAVRYLSTWFLMDVASTIPFDAIGYLITGTSTLNITCNLLGLLRFWRLRRVKHLFTRLEKDIRYSYFWIRCFRLLSVTLFLVHCAGCSYYLIADRYPHQGKTWTDAIPNFTETSLSIRYIAAIYWSITTMTTVGYGDLHASNTIEMVFITVYMLFNLGLTAYLIGNMTNLVVEGTRRTMEFRNSIEAASNFVNRNRLPPRLKDQILAYMCLRFKAESLNQQHLIDQLPKSIYKSICQHLFLPSVEKVYLFKGVSREILLLLVSKMKAEYIPPREDVIMQNEAPDDVYIIVSGEVEIIDSEMERESVLGTLRCGDIFGEVGALCCRPQSYTFQTKSLSQLLRLKTSFLIETMQIKQQDNATMLKNFLQHHKKLSNLDIGDLKAQQNGENTDVVPPNIASNLIAVVTTGNAALLDELLKAKLSPDITDSKGKTPLVTFCFHHKPFKTLTHNHHHSR

Target ORF information:

RefSeq Version	NM_001341531.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana potassium transport 2/3 (KT2/3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001341531.1

ORF Insert Sequence:

ATGGACCTCA AGTATTCAGC ATCTCATTGC AACTTATCCT CAGACATGAA GCTCAGGCGT 
TTTCATCAGC ATCGAGGAAA AGGAAGAGAA GAAGAGTATG ATGCTTCTTC TCTCAGCTTG 
AACAATCTGT CAAAACTTAT TCTTCCTCCA CTTGGTGTTG CTAGCTATAA CCAGAATCAC 
ATCAGGTCTA GTGGATGGAT CATCTCACCT ATGGACTCAA GATACAGGTG CTGGGAATTT 
TATATGGTGC TTTTAGTGGC ATACTCTGCG TGGGTTTACC CTTTTGAAGT TGCATTTCTG 
AATTCATCAC CAAAGAGAAA CCTTTGTATC GCGGACAACA TCGTAGACTT GTTCTTCGCG 
GTTGACATTG TCTTGACGTT TTTCGTTGCT TACATAGACG AAAGAACACA GCTTCTTGTC 
CGTGAACCTA AACAGATTGC AGTGAGGTAC CTATCAACAT GGTTCTTGAT GGATGTTGCA 
TCAACTATAC CATTTGACGC TATTGGATAC TTAATCACTG GCACATCCAC GTTAAATATC 
ACTTGTAATC TCTTGGGATT ACTTAGATTT TGGCGACTTC GAAGAGTTAA ACACCTCTTC 
ACTAGGCTCG AGAAGGACAT AAGATATAGC TATTTCTGGA TTCGCTGCTT TCGACTTCTA 
TCAGTGACAT TGTTTCTAGT GCACTGTGCT GGATGCAGTT ATTACCTAAT AGCAGACAGA 
TATCCACACC AAGGAAAGAC ATGGACTGAT GCGATCCCTA ATTTCACAGA GACAAGTCTT 
TCCATCAGAT ACATTGCAGC TATATATTGG TCTATCACTA CAATGACCAC AGTGGGATAT 
GGAGATCTTC ATGCAAGCAA CACTATTGAA ATGGTATTCA TAACAGTCTA CATGTTATTC 
AATCTTGGCC TCACTGCTTA CCTTATTGGT AACATGACTA ATTTGGTCGT GGAAGGGACT 
CGTCGTACCA TGGAATTTAG GAATAGCATT GAAGCAGCGT CAAACTTTGT TAACAGAAAC 
AGATTGCCTC CTAGATTAAA AGACCAGATA TTAGCTTACA TGTGTTTAAG GTTTAAAGCA 
GAGAGCTTAA ATCAGCAACA TCTTATTGAC CAGCTCCCAA AATCTATCTA CAAAAGCATT 
TGTCAACATC TTTTTCTTCC ATCTGTTGAA AAAGTTTACC TCTTCAAAGG CGTCTCAAGA 
GAAATACTTC TTCTTCTGGT TTCAAAAATG AAGGCTGAGT ATATACCACC AAGAGAGGAT 
GTCATTATGC AGAACGAAGC GCCGGATGAT GTTTACATAA TTGTGTCAGG AGAAGTTGAG 
ATCATTGATT CAGAGATGGA GAGAGAGTCT GTTTTAGGCA CTCTACGTTG TGGAGACATA 
TTTGGAGAAG TTGGAGCACT TTGTTGCAGA CCACAAAGCT ACACTTTTCA AACTAAGTCT 
TTATCACAGC TTCTCCGACT CAAAACATCT TTCCTTATTG AGACAATGCA GATTAAACAA 
CAAGACAATG CCACAATGCT CAAGAACTTC TTGCAGCATC ACAAAAAGCT GAGTAATTTA 
GACATTGGTG ATCTAAAGGC ACAACAAAAT GGCGAAAACA CCGATGTTGT TCCTCCTAAC 
ATTGCCTCAA ATCTCATCGC TGTGGTGACT ACAGGCAATG CAGCTCTTCT TGATGAGCTA 
CTTAAGGCTA AGTTAAGCCC TGACATTACA GATTCCAAAG GAAAAACTCC ATTGGTAACG 
TTTTGCTTTC ACCATAAGCC ATTTAAAACC CTTACTCATA ATCATCATCA CAGCCGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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KT2/3 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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