NCED4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NCED4
Entrez Gene ID	827655
Full Name	nine-cis-epoxycarotenoid dioxygenase 4
Synonyms	CCD4,T18B16.140,T18B16_140,carotenoid cleavage dioxygenase 4,nine-cis-epoxycarotenoid dioxygenase 4
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_118036.3	NP_193652.1	nine-cis-epoxycarotenoid dioxygenase 4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

enzymatic potential to form acyclic regulatory metabolites
Title: Enzymatic study on AtCCD4 and AtCCD7 and their potential to form acyclic regulatory metabolites.

CCD4 plays a major role in beta-carotene degradation in drying seeds and senescing leaves
Title: Carotenoid cleavage dioxygenase4 is a negative regulator of ?-carotene content in Arabidopsis seeds.

The following NCED4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCED4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb15366	NM_118036.3 Latest version!	Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 4 (NCED4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCED4 ( NM_118036.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_193652.1 Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 4 (NCED4), mRNA.

Price & Availability↑

CloneID	OAb15366
Clone ID Related Accession (Same CDS sequence)	NM_118036.3
Accession Version	NM_118036.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	nine-cis-epoxycarotenoid dioxygenase 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_118036.2.

ATGGACTCTG TTTCTTCTTC TTCCTTCCTC TCCTCCACAT TCTCTCTTCA TCACTCTCTT 
CTTCGCCGCC GATCTTCCTC TCCTACTCTC CTCCGTATCA ACTCCGCCGT CGTCGAAGAA 
CGTTCTCCAA TCACAAACCC AAGCGACAAC AATGATCGTC GTAACAAACC CAAAACACTC 
CACAACCGAA CCAATCACAC CTTAGTCTCA TCACCACCGA AACTCCGACC AGAAATGACT 
CTCGCAACAG CTCTCTTCAC CACCGTCGAA GATGTAATCA ACACGTTCAT CGATCCACCT 
TCACGTCCTT CCGTTGATCC AAAACATGTC CTCTCTGATA ACTTCGCTCC TGTCCTCGAC 
GAGCTTCCTC CAACAGACTG TGAAATCATC CACGGCACTC TTCCACTGTC ACTTAACGGC 
GCTTACATCC GTAACGGTCC AAATCCACAG TTTCTCCCTC GTGGTCCTTA CCATCTCTTC 
GACGGCGACG GTATGCTTCA CGCCATAAAA ATCCACAACG GTAAAGCCAC TCTCTGTAGC 
AGATACGTCA AGACTTATAA ATACAACGTC GAGAAACAAA CCGGAGCTCC GGTTATGCCT 
AACGTGTTTT CCGGATTCAA CGGTGTAACG GCGTCAGTAG CTCGTGGAGC TTTAACGGCA 
GCTAGGGTTT TAACCGGACA GTATAATCCG GTTAACGGCA TTGGTTTAGC TAATACAAGT 
CTAGCTTTCT TCAGTAACCG TCTCTTTGCT TTAGGTGAAT CTGATTTACC CTACGCCGTC 
CGATTAACCG AATCAGGAGA TATTGAAACG ATCGGACGGT ACGATTTCGA CGGGAAATTA 
GCGATGAGTA TGACAGCTCA TCCTAAAACC GATCCAATAA CCGGAGAAAC TTTCGCTTTC 
CGGTACGGTC CGGTTCCACC GTTTTTAACA TATTTCCGGT TTGATTCCGC CGGGAAAAAA 
CAAAGAGACG TTCCGATATT CTCGATGACG TCTCCGTCGT TTCTCCATGA CTTCGCGATC 
ACGAAACGTC ACGCGATTTT CGCAGAGATT CAGCTTGGCA TGAGGATGAA CATGTTGGAT 
TTGGTTCTCG AAGGTGGTTC TCCGGTTGGT ACTGATAACG GAAAAACTCC AAGGCTTGGA 
GTGATTCCTA AGTACGCCGG AGATGAGTCG GAGATGAAAT GGTTCGAAGT TCCTGGATTC 
AATATCATTC ACGCTATTAA TGCTTGGGAT GAAGATGATG GAAACAGCGT CGTTTTGATT 
GCACCGAATA TTATGTCGAT TGAACATACT TTAGAGAGGA TGGATCTGGT TCATGCTTTG 
GTGGAGAAGG TGAAGATCGA TCTCGTCACC GGGATTGTGA GACGTCATCC GATCTCAGCG 
AGGAATCTCG ATTTCGCTGT GATTAATCCG GCGTTTCTCG GGAGATGTAG CAGGTACGTT 
TACGCGGCGA TTGGAGATCC GATGCCGAAG ATCTCCGGTG TGGTGAAGCT TGATGTGTCT 
AAAGGAGATC GGGATGATTG TACGGTGGCC CGTAGAATGT ACGGTTCAGG TTGTTACGGC 
GGAGAACCGT TTTTCGTAGC TAGGGATCCT GGTAATCCGG AGGCGGAGGA GGATGATGGT 
TATGTGGTGA CGTATGTTCA CGATGAAGTG ACTGGAGAAT CGAAGTTTCT GGTGATGGAC 
GCTAAATCGC CGGAGCTTGA AATCGTCGCC GCCGTGAGGT TGCCGCGAAG GGTTCCGTAC 
GGATTCCATG GGTTATTTGT CAAGGAAAGT GACCTTAATA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_193652.1
CDS	217..2004
Translation	MDSVSSSSFLSSTFSLHHSLLRRRSSSPTLLRINSAVVEERSPITNPSDNNDRRNKPKTLHNRTNHTLVSSPPKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGTLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLERMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKISGVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARDPGNPEAEEDDGYVVTYVHDEVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL

Target ORF information:

RefSeq Version	NM_118036.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana nine-cis-epoxycarotenoid dioxygenase 4 (NCED4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_118036.3

ORF Insert Sequence:

ATGGACTCTG TTTCTTCTTC TTCCTTCCTC TCCTCCACAT TCTCTCTTCA TCACTCTCTT 
CTTCGCCGCC GATCTTCCTC TCCTACTCTC CTCCGTATCA ACTCCGCCGT CGTCGAAGAA 
CGTTCTCCAA TCACAAACCC AAGCGACAAC AATGATCGTC GTAACAAACC CAAAACACTC 
CACAACCGAA CCAATCACAC CTTAGTCTCA TCACCACCGA AACTCCGACC AGAAATGACT 
CTCGCAACAG CTCTCTTCAC CACCGTCGAA GATGTAATCA ACACGTTCAT CGATCCACCT 
TCACGTCCTT CCGTTGATCC AAAACATGTC CTCTCTGATA ACTTCGCTCC TGTCCTCGAC 
GAGCTTCCTC CAACAGACTG TGAAATCATC CACGGCACTC TTCCACTGTC ACTTAACGGC 
GCTTACATCC GTAACGGTCC AAATCCACAG TTTCTCCCTC GTGGTCCTTA CCATCTCTTC 
GACGGCGACG GTATGCTTCA CGCCATAAAA ATCCACAACG GTAAAGCCAC TCTCTGTAGC 
AGATACGTCA AGACTTATAA ATACAACGTC GAGAAACAAA CCGGAGCTCC GGTTATGCCT 
AACGTGTTTT CCGGATTCAA CGGTGTAACG GCGTCAGTAG CTCGTGGAGC TTTAACGGCA 
GCTAGGGTTT TAACCGGACA GTATAATCCG GTTAACGGCA TTGGTTTAGC TAATACAAGT 
CTAGCTTTCT TCAGTAACCG TCTCTTTGCT TTAGGTGAAT CTGATTTACC CTACGCCGTC 
CGATTAACCG AATCAGGAGA TATTGAAACG ATCGGACGGT ACGATTTCGA CGGGAAATTA 
GCGATGAGTA TGACAGCTCA TCCTAAAACC GATCCAATAA CCGGAGAAAC TTTCGCTTTC 
CGGTACGGTC CGGTTCCACC GTTTTTAACA TATTTCCGGT TTGATTCCGC CGGGAAAAAA 
CAAAGAGACG TTCCGATATT CTCGATGACG TCTCCGTCGT TTCTCCATGA CTTCGCGATC 
ACGAAACGTC ACGCGATTTT CGCAGAGATT CAGCTTGGCA TGAGGATGAA CATGTTGGAT 
TTGGTTCTCG AAGGTGGTTC TCCGGTTGGT ACTGATAACG GAAAAACTCC AAGGCTTGGA 
GTGATTCCTA AGTACGCCGG AGATGAGTCG GAGATGAAAT GGTTCGAAGT TCCTGGATTC 
AATATCATTC ACGCTATTAA TGCTTGGGAT GAAGATGATG GAAACAGCGT CGTTTTGATT 
GCACCGAATA TTATGTCGAT TGAACATACT TTAGAGAGGA TGGATCTGGT TCATGCTTTG 
GTGGAGAAGG TGAAGATCGA TCTCGTCACC GGGATTGTGA GACGTCATCC GATCTCAGCG 
AGGAATCTCG ATTTCGCTGT GATTAATCCG GCGTTTCTCG GGAGATGTAG CAGGTACGTT 
TACGCGGCGA TTGGAGATCC GATGCCGAAG ATCTCCGGTG TGGTGAAGCT TGATGTGTCT 
AAAGGAGATC GGGATGATTG TACGGTGGCC CGTAGAATGT ACGGTTCAGG TTGTTACGGC 
GGAGAACCGT TTTTCGTAGC TAGGGATCCT GGTAATCCGG AGGCGGAGGA GGATGATGGT 
TATGTGGTGA CGTATGTTCA CGATGAAGTG ACTGGAGAAT CGAAGTTTCT GGTGATGGAC 
GCTAAATCGC CGGAGCTTGA AATCGTCGCC GCCGTGAGGT TGCCGCGAAG GGTTCCGTAC 
GGATTCCATG GGTTATTTGT CAAGGAAAGT GACCTTAATA AGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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NCED4 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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