NIP5;1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIP5;1
Entrez Gene ID	826630
Full Name	NOD26-like intrinsic protein 5;1
Synonyms	AtNIP5;1,F7L13.6,NLM6,NLM8,NOD26-LIKE MIP 6,NOD26-LIKE MIP 8,NOD26-like intrinsic protein 5;1
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_117106.3	NP_192776.1	NOD26-like intrinsic protein 5;1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	NIP5;1	NP_192776.1
Oryza sativa (rice)	Os02g0745100	NP_001048108.1
Arabidopsis thaliana (thale cress)	NIP6;1	NP_178191.1
Oryza sativa (rice)	Os06g0228200	NP_001057207.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots.
Title: Polar Localization of the NIP5;1 Boric Acid Channel Is Maintained by Endocytosis and Facilitates Boron Transport in Arabidopsis Roots.

The direct root uptake of urea was increased under boron deficiency, in both the high and low affinity range. This activity was entirely mediated by the NIP5;1 channel.
Title: High and Low Affinity Urea Root Uptake: Involvement of NIP5;1.

Rough mapping and whole-genome sequencing identified the mutant with altered localization of NIP5;1 as an allele of UDP-glucose 4-epimerase 4.
Title: Identification and characterization of an Arabidopsis mutant with altered localization of NIP5;1, a plasma membrane boric acid channel, reveals the requirement for D-galactose in endomembrane organization.

The 5' UTR controls boron-dependent NIP5;1 mRNA degradation and that NIP5;1 mRNA degradation is important for plant acclimation to high-boron conditions.
Title: Boron-dependent degradation of NIP5;1 mRNA for acclimation to excess boron conditions in Arabidopsis.

The residue at the H5 helix position of the aromatic/arginine filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to silicon and boron, but there is a relatively low selectivity for arsenite.
Title: The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic.

The following NIP5;1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIP5;1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb14532	NM_117106.3 Latest version!	Arabidopsis thaliana NOD26-like intrinsic protein 5;1 (NIP5;1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIP5;1 ( NM_117106.3 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_192776.1 Arabidopsis thaliana NOD26-like intrinsic protein 5;1 (NIP5;1), mRNA.

Price & Availability↑

CloneID	OAb14532
Clone ID Related Accession (Same CDS sequence)	NM_117106.3
Accession Version	NM_117106.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	NOD26-like intrinsic protein 5;1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_117106.2.

ATGGCTCCAC CGGAGGCTGA GGTGGGTGCG GTAATGGTGA TGGCTCCTCC AACGCCGGGG 
ACACCGGGAA CACCGGGAGG ACCGTTGATC ACGGGGATGA GAGTGGATTC AATGTCGTTT 
GATCATCGGA AACCAACGCC TCGATGCAAA TGCTTGCCGG TGATGGGATC AACTTGGGGT 
CAACATGACA CATGCTTCAC CGATTTTCCC TCTCCTGATG TCTCCCTCAC TCGCAAGCTT 
GGAGCCGAGT TCGTGGGAAC ATTCATCTTG ATATTCACCG CGACAGCCGG TCCAATCGTG 
AACCAGAAAT ACGATGGAGC AGAAACCCTA ATCGGTAACG CTGCATGCGC AGGACTCGCA 
GTGATGATCA TAATACTCTC AACAGGTCAT ATCTCAGGGG CTCACCTAAA CCCATCACTG 
ACCATAGCAT TCGCTGCTCT AAGGCACTTC CCTTGGGCAC ACGTCCCTGC TTACATAGCG 
GCTCAAGTCT CAGCTTCCAT TTGCGCTTCA TTCGCACTTA AAGGAGTGTT CCATCCTTTT 
ATGTCCGGTG GTGTCACTAT TCCATCTGTT AGCCTTGGAC AAGCCTTTGC TCTTGAGTTC 
ATCATCACTT TCATTCTCCT CTTTGTTGTA ACTGCCGTCG CTACCGACAC TCGTGCCGTT 
GGAGAATTGG CAGGTATAGC CGTTGGAGCC ACGGTCATGC TCAATATTCT AGTCGCAGGG 
CCATCGACTG GTGGATCTAT GAATCCTGTG AGAACTCTAG GACCAGCCGT TGCATCAGGA 
AACTATAGGT CACTATGGGT GTATCTGGTG GCTCCTACAC TTGGTGCCAT ATCTGGTGCA 
GCGGTCTACA CAGGTGTTAA GCTTAACGAT AGCGTGACTG ACCCGCCTCG TCCGGTTAGG 
AGCTTTCGTC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_192776.1
CDS	887..1801
Translation	MAPPEAEVGAVMVMAPPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPVMGSTWGQHDTCFTDFPSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDPPRPVRSFRR

Target ORF information:

RefSeq Version	NM_117106.3
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana NOD26-like intrinsic protein 5;1 (NIP5;1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_117106.3

ORF Insert Sequence:

ATGGCTCCAC CGGAGGCTGA GGTGGGTGCG GTAATGGTGA TGGCTCCTCC AACGCCGGGG 
ACACCGGGAA CACCGGGAGG ACCGTTGATC ACGGGGATGA GAGTGGATTC AATGTCGTTT 
GATCATCGGA AACCAACGCC TCGATGCAAA TGCTTGCCGG TGATGGGATC AACTTGGGGT 
CAACATGACA CATGCTTCAC CGATTTTCCC TCTCCTGATG TCTCCCTCAC TCGCAAGCTT 
GGAGCCGAGT TCGTGGGAAC ATTCATCTTG ATATTCACCG CGACAGCCGG TCCAATCGTG 
AACCAGAAAT ACGATGGAGC AGAAACCCTA ATCGGTAACG CTGCATGCGC AGGACTCGCA 
GTGATGATCA TAATACTCTC AACAGGTCAT ATCTCAGGGG CTCACCTAAA CCCATCACTG 
ACCATAGCAT TCGCTGCTCT AAGGCACTTC CCTTGGGCAC ACGTCCCTGC TTACATAGCG 
GCTCAAGTCT CAGCTTCCAT TTGCGCTTCA TTCGCACTTA AAGGAGTGTT CCATCCTTTT 
ATGTCCGGTG GTGTCACTAT TCCATCTGTT AGCCTTGGAC AAGCCTTTGC TCTTGAGTTC 
ATCATCACTT TCATTCTCCT CTTTGTTGTA ACTGCCGTCG CTACCGACAC TCGTGCCGTT 
GGAGAATTGG CAGGTATAGC CGTTGGAGCC ACGGTCATGC TCAATATTCT AGTCGCAGGG 
CCATCGACTG GTGGATCTAT GAATCCTGTG AGAACTCTAG GACCAGCCGT TGCATCAGGA 
AACTATAGGT CACTATGGGT GTATCTGGTG GCTCCTACAC TTGGTGCCAT ATCTGGTGCA 
GCGGTCTACA CAGGTGTTAA GCTTAACGAT AGCGTGACTG ACCCGCCTCG TCCGGTTAGG 
AGCTTTCGTC GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
Mayer K,Sch?ller C,Wambutt R,Murphy G,Volckaert G,Pohl T,D?sterh?ft A,Stiekema W,Entian KD,Terryn N,Harris B,Ansorge W,Brandt P,Grivell L,Rieger M,Weichselgartner M,de Simone V,Obermaier B,Mache R,M?ller M,Kreis M,Delseny M,Puigdomenech P,Watson M,Schmidtheini T,Reichert B,Portatelle D,Perez-Alonso M,Boutry M,Bancroft I,Vos P,Hoheisel J,Zimmermann W,Wedler H,Ridley P,Langham SA,McCullagh B,Bilham L,Robben J,Van der Schueren J,Grymonprez B,Chuang YJ,Vandenbussche F,Braeken M,Weltjens I,Voet M,Bastiaens I,Aert R,Defoor E,Weitzenegger T,Bothe G,Ramsperger U,Hilbert H,Braun M,Holzer E,Brandt A,Peters S,van Staveren M,Dirske W,Mooijman P,Klein Lankhorst R,Rose M,Hauf J,K?tter P,Berneiser S,Hempel S,Feldpausch M,Lamberth S,Van den Daele H,De Keyser A,Buysshaert C,Gielen J,Villarroel R,De Clercq R,Van Montagu M,Rogers J,Cronin A,Quail M,Bray-Allen S,Clark L,Doggett J,Hall S,Kay M,Lennard N,McLay K,Mayes R,Pettett A,Rajandream MA,Lyne M,Benes V,Rechmann S,Borkova D,Bl?cker H,Scharfe M,Grimm M,L?hnert TH,Dose S,de Haan M,Maarse A,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,Fartmann B,Granderath K,Dauner D,Herzl A,Neumann S,Argiriou A,Vitale D,Liguori R,Piravandi E,Massenet O,Quigley F,Clabauld G,M?ndlein A,Felber R,Schnabl S,Hiller R,Schmidt W,Lecharny A,Aubourg S,Chefdor F,Cooke R,Berger C,Montfort A,Casacuberta E,Gibbons T,Weber N,Vandenbol M,Bargues M,Terol J,Torres A,Perez-Perez A,Purnelle B,Bent E,Johnson S,Tacon D,Jesse T,Heijnen L,Schwarz S,Scholler P,Heber S,Francs P,Bielke C,Frishman D,Haase D,Lemcke K,Mewes HW,Stocker S,Zaccaria P,Bevan M,Wilson RK,de la Bastide M,Habermann K,Parnell L,Dedhia N,Gnoj L,Schutz K,Huang E,Spiegel L,Sehkon M,Murray J,Sheet P,Cordes M,Abu-Threideh J,Stoneking T,Kalicki J,Graves T,Harmon G,Edwards J,Latreille P,Courtney L,Cloud J,Abbott A,Scott K,Johnson D,Minx P,Bentley D,Fulton B,Miller N,Greco T,Kemp K,Kramer J,Fulton L,Mardis E,Dante M,Pepin K,Hillier L,Nelson J,Spieth J,Ryan E,Andrews S,Geisel C,Layman D,Du H,Ali J,Berghoff A,Jones K,Drone K,Cotton M,Joshu C,Antonoiu B,Zidanic M,Strong C,Sun H,Lamar B,Yordan C,Ma P,Zhong J,Preston R,Vil D,Shekher M,Matero A,Shah R,Swaby IK,O'Shaughnessy A,Rodriguez M,Hoffmann J,Till S,Granat S,Shohdy N,Hasegawa A,Hameed A,Lodhi M,Johnson A,Chen E,Marra M,Martienssen R,McCombie WR

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NIP5;1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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