Gene Symbol | PHOT1 |
Entrez Gene ID | 823721 |
Full Name | phototropin 1 |
Synonyms | F16L2.3,JK224,NONPHOTOTROPIC HYPOCOTYL 1,NPH1,PHOTOTROPIN,ROOT PHOTOTROPISM 1,RPT1,phototropin 1 |
Gene Type | protein-coding |
Organism | Arabidopsis thaliana(Thale cress) |
ORF » Species Summary » Arabidopsis thaliana » PHOT1 cDNA ORF clone
Gene Symbol | PHOT1 |
Entrez Gene ID | 823721 |
Full Name | phototropin 1 |
Synonyms | F16L2.3,JK224,NONPHOTOTROPIC HYPOCOTYL 1,NPH1,PHOTOTROPIN,ROOT PHOTOTROPISM 1,RPT1,phototropin 1 |
Gene Type | protein-coding |
Organism | Arabidopsis thaliana(Thale cress) |
mRNA | Protein | Name |
---|---|---|
NM_001035737.1 | NP_001030814.1 | phototropin 1 |
NM_001035737.2 | NP_001030814.1 | phototropin 1 |
NM_114447.4 | NP_190164.1 | phototropin 1 |
NM_114447.3 | NP_190164.1 | phototropin 1 |
Oryza sativa (rice) | Os11g0102200 | NP_001065225.1 |
Arabidopsis thaliana (thale cress) | PHOT1 | NP_001030814.1 |
Oryza sativa (rice) | Os12g0101800 | NP_001065232.1 |
Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.
Salanoubat M, Lemcke K, Rieger M, Ansorge W, Unseld M, Fartmann B, Valle G, Bl?cker H, Perez-Alonso M, Obermaier B, Delseny M, Boutry M, Grivell LA, Mache R, Puigdom?nech P, De Simone V, Choisne N, Artiguenave F, Robert C, Brottier P, Wincker P, Cattolico L, Weissenbach J, Saurin W, Qu?tier F, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Benes V, Wurmbach E, Drzonek H, Erfle H, Jordan N, Bangert S, Wiedelmann R, Kranz H, Voss H, Holland R, Brandt P, Nyakatura G, Vezzi A, D'Angelo M, Pallavicini A, Toppo S, Simionati B, Conrad A, Hornischer K, Kauer G, L?hnert TH, Nordsiek G, Reichelt J, Scharfe M, Sch?n O, Bargues M, Terol J, Climent J, Navarro P, Collado C, Perez-Perez A, Ottenw?lder B, Duchemin D, Cooke R, Laudie M, Berger-Llauro C, Purnelle B, Masuy D, de Haan M, Maarse AC, Alcaraz JP, Cottet A, Casacuberta E, Monfort A, Argiriou A, flores M, Liguori R, Vitale D, Mannhaupt G, Haase D, Schoof H, Rudd S, Zaccaria P, Mewes HW, Mayer KF, Kaul S, Town CD, Koo HL, Tallon LJ, Jenkins J, Rooney T, Rizzo M, Walts A, Utterback T, Fujii CY, Shea TP, Creasy TH, Haas B, Maiti R, Wu D, Peterson J, Van Aken S, Pai G, Militscher J, Sellers P, Gill JE, Feldblyum TV, Preuss D, Lin X, Nierman WC, Salzberg SL, White O, Venter JC, Fraser CM, Kaneko T, Nakamura Y, Sato S, Kato T, Asamizu E, Sasamoto S, Kimura T, Idesawa K, Kawashima K, Kishida Y, Kiyokawa C, Kohara M, Matsumoto M, Matsuno A, Muraki A, Nakayama S, Nakazaki N, Shinpo S, Takeuchi C, Wada T, Watanabe A, Yamada M, Yasuda M, Tabata S, , ,
Nature408(6814)820-2(2000 Dec)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Data suggest that Arabidopsis can adapt to elevated phosphorylation status of phototropin-1 R472H mutant in part by reducing its stability, whereas activity of the mutant under high-light conditions can be attributed to additional increases in LOV2-mediated photoreceptor autophosphorylation. [phot1]
Title: Functional characterization of a constitutively active kinase variant of
The spatial targeting approach therefore excludes the hypocotyl apex as the site for light perception for phototropism and shows that phot1-mediated NPH3 de-phosphorylation is tissue autonomous and occurs more prominently in the basal hypocotyl.
Title: Functional characterization of Arabidopsis phototropin 1 in the hypocotyl apex.
lysine 475, a functionally important residue, in the N-terminal region of LOV2 plays a critical role in transmitting the structural changes in LOV2 to the kinase domain. The interface between the domains is critical for signaling, suitably changing the structure to activate the kinase in response to conformational changes in the adjoining LOV2.
Title: Blue Light-excited Light-Oxygen-Voltage-sensing Domain 2 (LOV2) Triggers a Rearrangement of the Kinase Domain to Induce Phosphorylation Activity in Arabidopsis Phototropin1.
phot1 and phot2 phosphorylate BLUE LIGHT SIGNALING1 (BLUS1) kinase as a common substrate in stomatal opening. phot1 phosphorylates BLUS1 more efficiently. The phosphorylation status of BLUS1 is a key determinant of physiological response.
Title: Arabidopsis phot1 and phot2 phosphorylate BLUS1 kinase with different efficiencies in stomatal opening.
After cold treatment, in darkness, the expression of phototropin1 is down-regulated, while phototropin2 levels are up-regulated. The motile system of chloroplasts in Arabidopsis is more sensitive to blue light at low temperature, similar to other species.
Title: The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana.
The following PHOT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PHOT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OAb12916 | |
Clone ID Related Accession (Same CDS sequence) | NM_001035737.1 , NM_001035737.2 , NM_114447.4 , NM_114447.3 | |
Accession Version | NM_001035737.1 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2014-01-21 | |
Organism | Arabidopsis thaliana(Thale cress) | |
Product | phototropin 1 | |
Comment |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001030814.1 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001035737.1 |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OAb12916 | |
Clone ID Related Accession (Same CDS sequence) | NM_001035737.1 , NM_001035737.2 , NM_114447.4 , NM_114447.3 | |
Accession Version | NM_001035737.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-13 | |
Organism | Arabidopsis thaliana(thale cress) | |
Product | phototropin 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_001035737.1. |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_001030814.1 |
CDS | 211..3201 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001035737.2 |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OAb12916 | |
Clone ID Related Accession (Same CDS sequence) | NM_001035737.1 , NM_001035737.2 , NM_114447.4 , NM_114447.3 | |
Accession Version | NM_114447.4 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-02-13 | |
Organism | Arabidopsis thaliana(thale cress) | |
Product | phototropin 1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003074). On Sep 12, 2016 this sequence version replaced NM_114447.3. |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_190164.1 |
CDS | 209..3199 |
Translation |
Target ORF information:
Target ORF information:
|
NM_114447.4 |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
CloneID | OAb12916 | |
Clone ID Related Accession (Same CDS sequence) | NM_001035737.1 , NM_001035737.2 , NM_114447.4 , NM_114447.3 | |
Accession Version | NM_114447.3 | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2014-01-21 | |
Organism | Arabidopsis thaliana(Thale cress) | |
Product | phototropin 1 | |
Comment |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_190164.1 |
Translation |
Target ORF information:
Target ORF information:
|
NM_114447.3 |
1 | ATGGAACCAA CAGAAAAACC ATCGACCAAA CCATCTTCTC GGACTCTACC TAGAGACACT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana. |