NCU11027 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	NCU11027
Entrez Gene ID	5847589
Full Name	zinc metalloprotease
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396489.1	XP_011394791.1	zinc metalloprotease, variant
XM_011396490.1	XP_011394792.1	zinc metalloprotease

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_02442	XP_003709231.1
Eremothecium gossypii	AGOS_AFR334W	NP_985881.1
Caenorhabditis elegans (roundworm)	C05D11.1	NP_001021145.1
Neurospora crassa	NCU11027	XP_001728321.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004747	XP_318071.4
Kluyveromyces lactis	KLLA0F27071g	XP_456284.1
Schizosaccharomyces pombe (fission yeast)	SPAC3H1.02c	NP_593544.1
Saccharomyces cerevisiae (baker's yeast)	YOL098C	NP_014543.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NCU11027 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU11027 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf09390	XM_011396489.1 Latest version!	Neurospora crassa OR74A zinc metalloprotease, variant (NCU11027), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ONf09390	XM_011396490.1 Latest version!	Neurospora crassa OR74A zinc metalloprotease (NCU11027), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NCU11027 ( XM_011396489.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394791.1 Neurospora crassa OR74A zinc metalloprotease, variant (NCU11027), mRNA.

Price & Availability↑

CloneID	ONf09390
Clone ID Related Accession (Same CDS sequence)	XM_011396489.1 , XM_011396490.1
Accession Version	XM_011396489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	zinc metalloprotease, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGTCGACAG CACCAAGGTT CCGCAAGGTG CAGACCTTCG AAACCGACTA TGCCCCGACG 
ACAATCACCC AGTACGTCTC AGAGCGTACT GGTATGCAGG TCATTGTGGC GGATCGCAAG 
GGTCCCAAAA TCAACGGTTA CTTTACCCTC GCTACCGAAA TCTTTGACGA TTCCGGAGCC 
CCTCACACTC TGGAACATCT GGTCTTCATG GGCTCCAAGA ACTACCAATA CAAGGGTCTC 
TTGGACAAGC TAGCTGGGCG TGCTTACTCT GGTACAAATG CATGGACTGC TGTTGACCAC 
ACAGCGTATA CGCTCGAGAC CGCCGGTTGG GACGGCTTTG CCCAGATCCT ACCCGTCTAT 
CTAGAGCATG TCATACTTCC TACCCTCACC GACGATGCGT GTGTGACGGA AGTTCACCAT 
ATCGACGGTG AGGGCAATGA TGCGGGTGTG GTATACTCCG AGATGCAGGC CATCCAATAC 
TCGAGTCAGG AGCTTATGGA TTTGCGGGCT CGCCGGTTGC TGTACCCCGA AAATGTGGGA 
TTTCGCTACG AAACGGGCGG TATGATGGAT GCCCTCAGGG TGCTGACCCC AGACCGCATC 
CGTGAGTTCC ACAAAGCCAT GTACCAACCT CAAAACCTGG CTGTCATCAT CGTCGGCGAA 
GCAAACCATG AGAATCTCTT GGACATTCTG GATAAGTTTG AGGAGAGCAT CAAGGACGAC 
ATCCCCCCTC CTAATCCTAA CTTCAAGCGC CCATTTGTCG ACTCTCCTCA ACCGCCTCCC 
CTGAAGGAAA CTATTGTCGA GACTGTTGAG TTTCCTGAGG AAGATGAGTC TACTGGCGAG 
ATTGTTGTGG CCTTCTTCGG TCCCAGCTGC GTTGACCAGT TGCAAGCAAC GGCTCTCAAC 
ATCCTCTTGA CATACCTCTG CGGCTCCTCC GTGTCTGTGA TCGAGAACAC TATTGTCGAA 
AAGGAGGAAT TGGCCAGCTC AGTTTCCTTC TGGTGGGATA CTCGCCCCAA CTCCGTTATC 
TGGTTCCAGC CAACCGGTGT CGCTACCGAG AAGCTCGCCT TTGTCGAGGA GCGCCTTATC 
TCGCTTCTGA AGGAGGTTGC ATCCAAGCCA CTCGATATGG AGTACATGAA GGAATGCATC 
TCGCGTGAGT TGCGTCAGAT CAAGTATCAT GCTGAAGGTT CGGAACAGTT CTACTCCAGC 
AATATCATCA CCGACTACTT GTTCGGAAAG CGTGACGGCT CTACCCTGAG GGAACTTCAG 
ACCTTGGGCG AGTACGATGT CCTCGCTAGC TGGGAAGAGC AGCAGTGGCG TGACTTCCTC 
AAGAAGTGGA TTTCCGACGC ACCTCACGTG TCCATCCTAG GCAAGCCCTC GATGGAACTC 
GCCAAAAAGC TCAAGAAGAC CGAGAAGGAG CGTCTCGCCA AGAGGAGGGA AGAGTTGGGA 
GAAGAGGGCC TGAAAGCACT GAAAGAAAAG GTTGAGAAGG CCAAGCAAAA CAACGAGAAG 
CCTATCCCTC CCGAGGTGCT TGATCAGTGG CCGGTTCCCG ACACATCATC CATCCACTTC 
ATTGAGTCGT TGACAGCAAG AAGCGGCAAG GCCCGCAGCC TCGGAACCAC CGATAACTCA 
ACCCAAAAGA TCATCGACGC AGCACCCCAA GGTGCTAAGC CTCTATTCAT CCAGTTTGAG 
GACGTACCTA CCAACTTTGT ACACCTTACG CTGCACATCG GAACGTCGGC GACGCCCAAC 
CGGCTCAAGC CTCTCCTCCC CCTGTTCGCA GACAATTTCT TCAACTCGCC GGTTATGCGC 
AATGGCAAAC GTATGGAGTT TGAAGACGTC GTCAAGCAAC TTGAGAAGGA TACCATCAGC 
TATCACCTGT CCAGTGCCTC GCGCATTGGT GATAACGAAG GTATGGCTCT GCAGTTCCAG 
GTGGACCTCG ACAAGTACAC CACTATGATC AGTTGGCTTC GCACACTGAT GTTTGACATC 
GTTTTCGATC CCGTCCGTCT CAAGGCCGCT GTTGTCAAGG CTCTTGCCGA TATTCCTGAG 
GCCAAGAGAG ACGGCAGAGT CATGTCTCGC GAAGTCGATC AGTCCATCCA TCTACTGCCG 
GAATCTTACT CTGTCGCTAA GCGGACGCTC GTCAAGGCGG TATACTACCG TCGCCTAAAG 
AAACTTCTCG AGAAGGAACC CGAGACCGTC GTCTCCTGGT TCGAGGAACT CCGCCGTAGT 
CTCTTCACCT TCGAGAACCT CAGAGTCCTT GTCACAGCCC ACGTCGAGAA GCTTCCCAAC 
CCAATCGCGG CCTGGGACAG CTTCACCGAG ATCCCCGACC TCGACACCTC CAAGGATCTC 
CTGCCCGTTG TCCGCCCGCA CCAGATGCTG AGCGACGAGG GCAAAGCCCC GGGCTCCTTC 
GGCAGTGTCA TCATCCCCAT GACCACGCTC GACTCCTCCT TCAGCGTCAG CACCGCCAGC 
GGCATCAAGT CCTACTCGGA TCCTCGCTTG CCCGCCTACC TGGTCGCCCT CTCCTACCTT 
GAGACCACCG AGGGCCCCCT TTGGAACGCC GTCCGTGGCA ATGGTCTCGC CTACGGCGTC 
CACTTTAGCC GTGAGTCCGA CTCGGGCTAC ATCCAGTTCC ACGTGTACCG GTCACCCGAC 
GCCAGCAAGG CTATCGAGGC CAGCAGGAAC ATGGTGGCCA AGATTGCTTC CGGAGAGGAG 
CCGCTGGACA AGCACCTAGT TGAGGGCGCC ATCAGCGGCC TGGTGCTGGC TGTTGCAGAC 
GAGCAGGCTA CCATGACGGC GGCGGCGCAG CAGAACTTTG TCAGCAACGT CATTCGCGGC 
CAGGAGCCCG GTTGGAACAA GAAGCTTCTG GCGCGCATTC GTGAGGTGAC GGGTGATCAG 
ATTCGCGAGG CTTTGCGGGA GATTGTGTTG CCTGTCTTTG AGCCGGGCAA GAGTAACGTT 
GTGGTTACTT GTGCTCCCAT TATGGAAGAG AACATGGTCA AGAATCTTGA GGCTATTGGC 
TACAAGACTC AAGTTCAGCC TCTTACGCAC TTCTATGATG ACTACGGCCT AAAGGCCGAC 
GAAGATGAGG AGGAAGAAGA GGAGGAAGAG GAGGATGAAG ATGATGACCA TCACCACCAT 
CACGGCTGCG GAAGCGAGTG CGAGAGCGAG TGCGGAAGTG AGTGTGACTC GGACGAGGAT 
ATGTCGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394791.1
CDS	330..3521
Translation	MSTAPRFRKVQTFETDYAPTTITQYVSERTGMQVIVADRKGPKINGYFTLATEIFDDSGAPHTLEHLVFMGSKNYQYKGLLDKLAGRAYSGTNAWTAVDHTAYTLETAGWDGFAQILPVYLEHVILPTLTDDACVTEVHHIDGEGNDAGVVYSEMQAIQYSSQELMDLRARRLLYPENVGFRYETGGMMDALRVLTPDRIREFHKAMYQPQNLAVIIVGEANHENLLDILDKFEESIKDDIPPPNPNFKRPFVDSPQPPPLKETIVETVEFPEEDESTGEIVVAFFGPSCVDQLQATALNILLTYLCGSSVSVIENTIVEKEELASSVSFWWDTRPNSVIWFQPTGVATEKLAFVEERLISLLKEVASKPLDMEYMKECISRELRQIKYHAEGSEQFYSSNIITDYLFGKRDGSTLRELQTLGEYDVLASWEEQQWRDFLKKWISDAPHVSILGKPSMELAKKLKKTEKERLAKRREELGEEGLKALKEKVEKAKQNNEKPIPPEVLDQWPVPDTSSIHFIESLTARSGKARSLGTTDNSTQKIIDAAPQGAKPLFIQFEDVPTNFVHLTLHIGTSATPNRLKPLLPLFADNFFNSPVMRNGKRMEFEDVVKQLEKDTISYHLSSASRIGDNEGMALQFQVDLDKYTTMISWLRTLMFDIVFDPVRLKAAVVKALADIPEAKRDGRVMSREVDQSIHLLPESYSVAKRTLVKAVYYRRLKKLLEKEPETVVSWFEELRRSLFTFENLRVLVTAHVEKLPNPIAAWDSFTEIPDLDTSKDLLPVVRPHQMLSDEGKAPGSFGSVIIPMTTLDSSFSVSTASGIKSYSDPRLPAYLVALSYLETTEGPLWNAVRGNGLAYGVHFSRESDSGYIQFHVYRSPDASKAIEASRNMVAKIASGEEPLDKHLVEGAISGLVLAVADEQATMTAAAQQNFVSNVIRGQEPGWNKKLLARIREVTGDQIREALREIVLPVFEPGKSNVVVTCAPIMEENMVKNLEAIGYKTQVQPLTHFYDDYGLKADEDEEEEEEEEEDEDDDHHHHHGCGSECESECGSECDSDEDMSD

Target ORF information:

RefSeq Version	XM_011396489.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A zinc metalloprotease, variant (NCU11027), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396489.1

ORF Insert Sequence:

ATGTCGACAG CACCAAGGTT CCGCAAGGTG CAGACCTTCG AAACCGACTA TGCCCCGACG 
ACAATCACCC AGTACGTCTC AGAGCGTACT GGTATGCAGG TCATTGTGGC GGATCGCAAG 
GGTCCCAAAA TCAACGGTTA CTTTACCCTC GCTACCGAAA TCTTTGACGA TTCCGGAGCC 
CCTCACACTC TGGAACATCT GGTCTTCATG GGCTCCAAGA ACTACCAATA CAAGGGTCTC 
TTGGACAAGC TAGCTGGGCG TGCTTACTCT GGTACAAATG CATGGACTGC TGTTGACCAC 
ACAGCGTATA CGCTCGAGAC CGCCGGTTGG GACGGCTTTG CCCAGATCCT ACCCGTCTAT 
CTAGAGCATG TCATACTTCC TACCCTCACC GACGATGCGT GTGTGACGGA AGTTCACCAT 
ATCGACGGTG AGGGCAATGA TGCGGGTGTG GTATACTCCG AGATGCAGGC CATCCAATAC 
TCGAGTCAGG AGCTTATGGA TTTGCGGGCT CGCCGGTTGC TGTACCCCGA AAATGTGGGA 
TTTCGCTACG AAACGGGCGG TATGATGGAT GCCCTCAGGG TGCTGACCCC AGACCGCATC 
CGTGAGTTCC ACAAAGCCAT GTACCAACCT CAAAACCTGG CTGTCATCAT CGTCGGCGAA 
GCAAACCATG AGAATCTCTT GGACATTCTG GATAAGTTTG AGGAGAGCAT CAAGGACGAC 
ATCCCCCCTC CTAATCCTAA CTTCAAGCGC CCATTTGTCG ACTCTCCTCA ACCGCCTCCC 
CTGAAGGAAA CTATTGTCGA GACTGTTGAG TTTCCTGAGG AAGATGAGTC TACTGGCGAG 
ATTGTTGTGG CCTTCTTCGG TCCCAGCTGC GTTGACCAGT TGCAAGCAAC GGCTCTCAAC 
ATCCTCTTGA CATACCTCTG CGGCTCCTCC GTGTCTGTGA TCGAGAACAC TATTGTCGAA 
AAGGAGGAAT TGGCCAGCTC AGTTTCCTTC TGGTGGGATA CTCGCCCCAA CTCCGTTATC 
TGGTTCCAGC CAACCGGTGT CGCTACCGAG AAGCTCGCCT TTGTCGAGGA GCGCCTTATC 
TCGCTTCTGA AGGAGGTTGC ATCCAAGCCA CTCGATATGG AGTACATGAA GGAATGCATC 
TCGCGTGAGT TGCGTCAGAT CAAGTATCAT GCTGAAGGTT CGGAACAGTT CTACTCCAGC 
AATATCATCA CCGACTACTT GTTCGGAAAG CGTGACGGCT CTACCCTGAG GGAACTTCAG 
ACCTTGGGCG AGTACGATGT CCTCGCTAGC TGGGAAGAGC AGCAGTGGCG TGACTTCCTC 
AAGAAGTGGA TTTCCGACGC ACCTCACGTG TCCATCCTAG GCAAGCCCTC GATGGAACTC 
GCCAAAAAGC TCAAGAAGAC CGAGAAGGAG CGTCTCGCCA AGAGGAGGGA AGAGTTGGGA 
GAAGAGGGCC TGAAAGCACT GAAAGAAAAG GTTGAGAAGG CCAAGCAAAA CAACGAGAAG 
CCTATCCCTC CCGAGGTGCT TGATCAGTGG CCGGTTCCCG ACACATCATC CATCCACTTC 
ATTGAGTCGT TGACAGCAAG AAGCGGCAAG GCCCGCAGCC TCGGAACCAC CGATAACTCA 
ACCCAAAAGA TCATCGACGC AGCACCCCAA GGTGCTAAGC CTCTATTCAT CCAGTTTGAG 
GACGTACCTA CCAACTTTGT ACACCTTACG CTGCACATCG GAACGTCGGC GACGCCCAAC 
CGGCTCAAGC CTCTCCTCCC CCTGTTCGCA GACAATTTCT TCAACTCGCC GGTTATGCGC 
AATGGCAAAC GTATGGAGTT TGAAGACGTC GTCAAGCAAC TTGAGAAGGA TACCATCAGC 
TATCACCTGT CCAGTGCCTC GCGCATTGGT GATAACGAAG GTATGGCTCT GCAGTTCCAG 
GTGGACCTCG ACAAGTACAC CACTATGATC AGTTGGCTTC GCACACTGAT GTTTGACATC 
GTTTTCGATC CCGTCCGTCT CAAGGCCGCT GTTGTCAAGG CTCTTGCCGA TATTCCTGAG 
GCCAAGAGAG ACGGCAGAGT CATGTCTCGC GAAGTCGATC AGTCCATCCA TCTACTGCCG 
GAATCTTACT CTGTCGCTAA GCGGACGCTC GTCAAGGCGG TATACTACCG TCGCCTAAAG 
AAACTTCTCG AGAAGGAACC CGAGACCGTC GTCTCCTGGT TCGAGGAACT CCGCCGTAGT 
CTCTTCACCT TCGAGAACCT CAGAGTCCTT GTCACAGCCC ACGTCGAGAA GCTTCCCAAC 
CCAATCGCGG CCTGGGACAG CTTCACCGAG ATCCCCGACC TCGACACCTC CAAGGATCTC 
CTGCCCGTTG TCCGCCCGCA CCAGATGCTG AGCGACGAGG GCAAAGCCCC GGGCTCCTTC 
GGCAGTGTCA TCATCCCCAT GACCACGCTC GACTCCTCCT TCAGCGTCAG CACCGCCAGC 
GGCATCAAGT CCTACTCGGA TCCTCGCTTG CCCGCCTACC TGGTCGCCCT CTCCTACCTT 
GAGACCACCG AGGGCCCCCT TTGGAACGCC GTCCGTGGCA ATGGTCTCGC CTACGGCGTC 
CACTTTAGCC GTGAGTCCGA CTCGGGCTAC ATCCAGTTCC ACGTGTACCG GTCACCCGAC 
GCCAGCAAGG CTATCGAGGC CAGCAGGAAC ATGGTGGCCA AGATTGCTTC CGGAGAGGAG 
CCGCTGGACA AGCACCTAGT TGAGGGCGCC ATCAGCGGCC TGGTGCTGGC TGTTGCAGAC 
GAGCAGGCTA CCATGACGGC GGCGGCGCAG CAGAACTTTG TCAGCAACGT CATTCGCGGC 
CAGGAGCCCG GTTGGAACAA GAAGCTTCTG GCGCGCATTC GTGAGGTGAC GGGTGATCAG 
ATTCGCGAGG CTTTGCGGGA GATTGTGTTG CCTGTCTTTG AGCCGGGCAA GAGTAACGTT 
GTGGTTACTT GTGCTCCCAT TATGGAAGAG AACATGGTCA AGAATCTTGA GGCTATTGGC 
TACAAGACTC AAGTTCAGCC TCTTACGCAC TTCTATGATG ACTACGGCCT AAAGGCCGAC 
GAAGATGAGG AGGAAGAAGA GGAGGAAGAG GAGGATGAAG ATGATGACCA TCACCACCAT 
CACGGCTGCG GAAGCGAGTG CGAGAGCGAG TGCGGAAGTG AGTGTGACTC GGACGAGGAT 
ATGTCGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NCU11027 ( XM_011396490.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394792.1 Neurospora crassa OR74A zinc metalloprotease (NCU11027), mRNA.

Price & Availability↑

CloneID	ONf09390
Clone ID Related Accession (Same CDS sequence)	XM_011396489.1 , XM_011396490.1
Accession Version	XM_011396490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	zinc metalloprotease
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGTCGACAG CACCAAGGTT CCGCAAGGTG CAGACCTTCG AAACCGACTA TGCCCCGACG 
ACAATCACCC AGTACGTCTC AGAGCGTACT GGTATGCAGG TCATTGTGGC GGATCGCAAG 
GGTCCCAAAA TCAACGGTTA CTTTACCCTC GCTACCGAAA TCTTTGACGA TTCCGGAGCC 
CCTCACACTC TGGAACATCT GGTCTTCATG GGCTCCAAGA ACTACCAATA CAAGGGTCTC 
TTGGACAAGC TAGCTGGGCG TGCTTACTCT GGTACAAATG CATGGACTGC TGTTGACCAC 
ACAGCGTATA CGCTCGAGAC CGCCGGTTGG GACGGCTTTG CCCAGATCCT ACCCGTCTAT 
CTAGAGCATG TCATACTTCC TACCCTCACC GACGATGCGT GTGTGACGGA AGTTCACCAT 
ATCGACGGTG AGGGCAATGA TGCGGGTGTG GTATACTCCG AGATGCAGGC CATCCAATAC 
TCGAGTCAGG AGCTTATGGA TTTGCGGGCT CGCCGGTTGC TGTACCCCGA AAATGTGGGA 
TTTCGCTACG AAACGGGCGG TATGATGGAT GCCCTCAGGG TGCTGACCCC AGACCGCATC 
CGTGAGTTCC ACAAAGCCAT GTACCAACCT CAAAACCTGG CTGTCATCAT CGTCGGCGAA 
GCAAACCATG AGAATCTCTT GGACATTCTG GATAAGTTTG AGGAGAGCAT CAAGGACGAC 
ATCCCCCCTC CTAATCCTAA CTTCAAGCGC CCATTTGTCG ACTCTCCTCA ACCGCCTCCC 
CTGAAGGAAA CTATTGTCGA GACTGTTGAG TTTCCTGAGG AAGATGAGTC TACTGGCGAG 
ATTGTTGTGG CCTTCTTCGG TCCCAGCTGC GTTGACCAGT TGCAAGCAAC GGCTCTCAAC 
ATCCTCTTGA CATACCTCTG CGGCTCCTCC GTGTCTGTGA TCGAGAACAC TATTGTCGAA 
AAGGAGGAAT TGGCCAGCTC AGTTTCCTTC TGGTGGGATA CTCGCCCCAA CTCCGTTATC 
TGGTTCCAGC CAACCGGTGT CGCTACCGAG AAGCTCGCCT TTGTCGAGGA GCGCCTTATC 
TCGCTTCTGA AGGAGGTTGC ATCCAAGCCA CTCGATATGG AGTACATGAA GGAATGCATC 
TCGCGTGAGT TGCGTCAGAT CAAGTATCAT GCTGAAGGTT CGGAACAGTT CTACTCCAGC 
AATATCATCA CCGACTACTT GTTCGGAAAG CGTGACGGCT CTACCCTGAG GGAACTTCAG 
ACCTTGGGCG AGTACGATGT CCTCGCTAGC TGGGAAGAGC AGCAGTGGCG TGACTTCCTC 
AAGAAGTGGA TTTCCGACGC ACCTCACGTG TCCATCCTAG GCAAGCCCTC GATGGAACTC 
GCCAAAAAGC TCAAGAAGAC CGAGAAGGAG CGTCTCGCCA AGAGGAGGGA AGAGTTGGGA 
GAAGAGGGCC TGAAAGCACT GAAAGAAAAG GTTGAGAAGG CCAAGCAAAA CAACGAGAAG 
CCTATCCCTC CCGAGGTGCT TGATCAGTGG CCGGTTCCCG ACACATCATC CATCCACTTC 
ATTGAGTCGT TGACAGCAAG AAGCGGCAAG GCCCGCAGCC TCGGAACCAC CGATAACTCA 
ACCCAAAAGA TCATCGACGC AGCACCCCAA GGTGCTAAGC CTCTATTCAT CCAGTTTGAG 
GACGTACCTA CCAACTTTGT ACACCTTACG CTGCACATCG GAACGTCGGC GACGCCCAAC 
CGGCTCAAGC CTCTCCTCCC CCTGTTCGCA GACAATTTCT TCAACTCGCC GGTTATGCGC 
AATGGCAAAC GTATGGAGTT TGAAGACGTC GTCAAGCAAC TTGAGAAGGA TACCATCAGC 
TATCACCTGT CCAGTGCCTC GCGCATTGGT GATAACGAAG GTATGGCTCT GCAGTTCCAG 
GTGGACCTCG ACAAGTACAC CACTATGATC AGTTGGCTTC GCACACTGAT GTTTGACATC 
GTTTTCGATC CCGTCCGTCT CAAGGCCGCT GTTGTCAAGG CTCTTGCCGA TATTCCTGAG 
GCCAAGAGAG ACGGCAGAGT CATGTCTCGC GAAGTCGATC AGTCCATCCA TCTACTGCCG 
GAATCTTACT CTGTCGCTAA GCGGACGCTC GTCAAGGCGG TATACTACCG TCGCCTAAAG 
AAACTTCTCG AGAAGGAACC CGAGACCGTC GTCTCCTGGT TCGAGGAACT CCGCCGTAGT 
CTCTTCACCT TCGAGAACCT CAGAGTCCTT GTCACAGCCC ACGTCGAGAA GCTTCCCAAC 
CCAATCGCGG CCTGGGACAG CTTCACCGAG ATCCCCGACC TCGACACCTC CAAGGATCTC 
CTGCCCGTTG TCCGCCCGCA CCAGATGCTG AGCGACGAGG GCAAAGCCCC GGGCTCCTTC 
GGCAGTGTCA TCATCCCCAT GACCACGCTC GACTCCTCCT TCAGCGTCAG CACCGCCAGC 
GGCATCAAGT CCTACTCGGA TCCTCGCTTG CCCGCCTACC TGGTCGCCCT CTCCTACCTT 
GAGACCACCG AGGGCCCCCT TTGGAACGCC GTCCGTGGCA ATGGTCTCGC CTACGGCGTC 
CACTTTAGCC GTGAGTCCGA CTCGGGCTAC ATCCAGTTCC ACGTGTACCG GTCACCCGAC 
GCCAGCAAGG CTATCGAGGC CAGCAGGAAC ATGGTGGCCA AGATTGCTTC CGGAGAGGAG 
CCGCTGGACA AGCACCTAGT TGAGGGCGCC ATCAGCGGCC TGGTGCTGGC TGTTGCAGAC 
GAGCAGGCTA CCATGACGGC GGCGGCGCAG CAGAACTTTG TCAGCAACGT CATTCGCGGC 
CAGGAGCCCG GTTGGAACAA GAAGCTTCTG GCGCGCATTC GTGAGGTGAC GGGTGATCAG 
ATTCGCGAGG CTTTGCGGGA GATTGTGTTG CCTGTCTTTG AGCCGGGCAA GAGTAACGTT 
GTGGTTACTT GTGCTCCCAT TATGGAAGAG AACATGGTCA AGAATCTTGA GGCTATTGGC 
TACAAGACTC AAGTTCAGCC TCTTACGCAC TTCTATGATG ACTACGGCCT AAAGGCCGAC 
GAAGATGAGG AGGAAGAAGA GGAGGAAGAG GAGGATGAAG ATGATGACCA TCACCACCAT 
CACGGCTGCG GAAGCGAGTG CGAGAGCGAG TGCGGAAGTG AGTGTGACTC GGACGAGGAT 
ATGTCGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394792.1
CDS	560..3751
Translation	MSTAPRFRKVQTFETDYAPTTITQYVSERTGMQVIVADRKGPKINGYFTLATEIFDDSGAPHTLEHLVFMGSKNYQYKGLLDKLAGRAYSGTNAWTAVDHTAYTLETAGWDGFAQILPVYLEHVILPTLTDDACVTEVHHIDGEGNDAGVVYSEMQAIQYSSQELMDLRARRLLYPENVGFRYETGGMMDALRVLTPDRIREFHKAMYQPQNLAVIIVGEANHENLLDILDKFEESIKDDIPPPNPNFKRPFVDSPQPPPLKETIVETVEFPEEDESTGEIVVAFFGPSCVDQLQATALNILLTYLCGSSVSVIENTIVEKEELASSVSFWWDTRPNSVIWFQPTGVATEKLAFVEERLISLLKEVASKPLDMEYMKECISRELRQIKYHAEGSEQFYSSNIITDYLFGKRDGSTLRELQTLGEYDVLASWEEQQWRDFLKKWISDAPHVSILGKPSMELAKKLKKTEKERLAKRREELGEEGLKALKEKVEKAKQNNEKPIPPEVLDQWPVPDTSSIHFIESLTARSGKARSLGTTDNSTQKIIDAAPQGAKPLFIQFEDVPTNFVHLTLHIGTSATPNRLKPLLPLFADNFFNSPVMRNGKRMEFEDVVKQLEKDTISYHLSSASRIGDNEGMALQFQVDLDKYTTMISWLRTLMFDIVFDPVRLKAAVVKALADIPEAKRDGRVMSREVDQSIHLLPESYSVAKRTLVKAVYYRRLKKLLEKEPETVVSWFEELRRSLFTFENLRVLVTAHVEKLPNPIAAWDSFTEIPDLDTSKDLLPVVRPHQMLSDEGKAPGSFGSVIIPMTTLDSSFSVSTASGIKSYSDPRLPAYLVALSYLETTEGPLWNAVRGNGLAYGVHFSRESDSGYIQFHVYRSPDASKAIEASRNMVAKIASGEEPLDKHLVEGAISGLVLAVADEQATMTAAAQQNFVSNVIRGQEPGWNKKLLARIREVTGDQIREALREIVLPVFEPGKSNVVVTCAPIMEENMVKNLEAIGYKTQVQPLTHFYDDYGLKADEDEEEEEEEEEDEDDDHHHHHGCGSECESECGSECDSDEDMSD

Target ORF information:

RefSeq Version	XM_011396490.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A zinc metalloprotease (NCU11027), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396490.1

ORF Insert Sequence:

ATGTCGACAG CACCAAGGTT CCGCAAGGTG CAGACCTTCG AAACCGACTA TGCCCCGACG 
ACAATCACCC AGTACGTCTC AGAGCGTACT GGTATGCAGG TCATTGTGGC GGATCGCAAG 
GGTCCCAAAA TCAACGGTTA CTTTACCCTC GCTACCGAAA TCTTTGACGA TTCCGGAGCC 
CCTCACACTC TGGAACATCT GGTCTTCATG GGCTCCAAGA ACTACCAATA CAAGGGTCTC 
TTGGACAAGC TAGCTGGGCG TGCTTACTCT GGTACAAATG CATGGACTGC TGTTGACCAC 
ACAGCGTATA CGCTCGAGAC CGCCGGTTGG GACGGCTTTG CCCAGATCCT ACCCGTCTAT 
CTAGAGCATG TCATACTTCC TACCCTCACC GACGATGCGT GTGTGACGGA AGTTCACCAT 
ATCGACGGTG AGGGCAATGA TGCGGGTGTG GTATACTCCG AGATGCAGGC CATCCAATAC 
TCGAGTCAGG AGCTTATGGA TTTGCGGGCT CGCCGGTTGC TGTACCCCGA AAATGTGGGA 
TTTCGCTACG AAACGGGCGG TATGATGGAT GCCCTCAGGG TGCTGACCCC AGACCGCATC 
CGTGAGTTCC ACAAAGCCAT GTACCAACCT CAAAACCTGG CTGTCATCAT CGTCGGCGAA 
GCAAACCATG AGAATCTCTT GGACATTCTG GATAAGTTTG AGGAGAGCAT CAAGGACGAC 
ATCCCCCCTC CTAATCCTAA CTTCAAGCGC CCATTTGTCG ACTCTCCTCA ACCGCCTCCC 
CTGAAGGAAA CTATTGTCGA GACTGTTGAG TTTCCTGAGG AAGATGAGTC TACTGGCGAG 
ATTGTTGTGG CCTTCTTCGG TCCCAGCTGC GTTGACCAGT TGCAAGCAAC GGCTCTCAAC 
ATCCTCTTGA CATACCTCTG CGGCTCCTCC GTGTCTGTGA TCGAGAACAC TATTGTCGAA 
AAGGAGGAAT TGGCCAGCTC AGTTTCCTTC TGGTGGGATA CTCGCCCCAA CTCCGTTATC 
TGGTTCCAGC CAACCGGTGT CGCTACCGAG AAGCTCGCCT TTGTCGAGGA GCGCCTTATC 
TCGCTTCTGA AGGAGGTTGC ATCCAAGCCA CTCGATATGG AGTACATGAA GGAATGCATC 
TCGCGTGAGT TGCGTCAGAT CAAGTATCAT GCTGAAGGTT CGGAACAGTT CTACTCCAGC 
AATATCATCA CCGACTACTT GTTCGGAAAG CGTGACGGCT CTACCCTGAG GGAACTTCAG 
ACCTTGGGCG AGTACGATGT CCTCGCTAGC TGGGAAGAGC AGCAGTGGCG TGACTTCCTC 
AAGAAGTGGA TTTCCGACGC ACCTCACGTG TCCATCCTAG GCAAGCCCTC GATGGAACTC 
GCCAAAAAGC TCAAGAAGAC CGAGAAGGAG CGTCTCGCCA AGAGGAGGGA AGAGTTGGGA 
GAAGAGGGCC TGAAAGCACT GAAAGAAAAG GTTGAGAAGG CCAAGCAAAA CAACGAGAAG 
CCTATCCCTC CCGAGGTGCT TGATCAGTGG CCGGTTCCCG ACACATCATC CATCCACTTC 
ATTGAGTCGT TGACAGCAAG AAGCGGCAAG GCCCGCAGCC TCGGAACCAC CGATAACTCA 
ACCCAAAAGA TCATCGACGC AGCACCCCAA GGTGCTAAGC CTCTATTCAT CCAGTTTGAG 
GACGTACCTA CCAACTTTGT ACACCTTACG CTGCACATCG GAACGTCGGC GACGCCCAAC 
CGGCTCAAGC CTCTCCTCCC CCTGTTCGCA GACAATTTCT TCAACTCGCC GGTTATGCGC 
AATGGCAAAC GTATGGAGTT TGAAGACGTC GTCAAGCAAC TTGAGAAGGA TACCATCAGC 
TATCACCTGT CCAGTGCCTC GCGCATTGGT GATAACGAAG GTATGGCTCT GCAGTTCCAG 
GTGGACCTCG ACAAGTACAC CACTATGATC AGTTGGCTTC GCACACTGAT GTTTGACATC 
GTTTTCGATC CCGTCCGTCT CAAGGCCGCT GTTGTCAAGG CTCTTGCCGA TATTCCTGAG 
GCCAAGAGAG ACGGCAGAGT CATGTCTCGC GAAGTCGATC AGTCCATCCA TCTACTGCCG 
GAATCTTACT CTGTCGCTAA GCGGACGCTC GTCAAGGCGG TATACTACCG TCGCCTAAAG 
AAACTTCTCG AGAAGGAACC CGAGACCGTC GTCTCCTGGT TCGAGGAACT CCGCCGTAGT 
CTCTTCACCT TCGAGAACCT CAGAGTCCTT GTCACAGCCC ACGTCGAGAA GCTTCCCAAC 
CCAATCGCGG CCTGGGACAG CTTCACCGAG ATCCCCGACC TCGACACCTC CAAGGATCTC 
CTGCCCGTTG TCCGCCCGCA CCAGATGCTG AGCGACGAGG GCAAAGCCCC GGGCTCCTTC 
GGCAGTGTCA TCATCCCCAT GACCACGCTC GACTCCTCCT TCAGCGTCAG CACCGCCAGC 
GGCATCAAGT CCTACTCGGA TCCTCGCTTG CCCGCCTACC TGGTCGCCCT CTCCTACCTT 
GAGACCACCG AGGGCCCCCT TTGGAACGCC GTCCGTGGCA ATGGTCTCGC CTACGGCGTC 
CACTTTAGCC GTGAGTCCGA CTCGGGCTAC ATCCAGTTCC ACGTGTACCG GTCACCCGAC 
GCCAGCAAGG CTATCGAGGC CAGCAGGAAC ATGGTGGCCA AGATTGCTTC CGGAGAGGAG 
CCGCTGGACA AGCACCTAGT TGAGGGCGCC ATCAGCGGCC TGGTGCTGGC TGTTGCAGAC 
GAGCAGGCTA CCATGACGGC GGCGGCGCAG CAGAACTTTG TCAGCAACGT CATTCGCGGC 
CAGGAGCCCG GTTGGAACAA GAAGCTTCTG GCGCGCATTC GTGAGGTGAC GGGTGATCAG 
ATTCGCGAGG CTTTGCGGGA GATTGTGTTG CCTGTCTTTG AGCCGGGCAA GAGTAACGTT 
GTGGTTACTT GTGCTCCCAT TATGGAAGAG AACATGGTCA AGAATCTTGA GGCTATTGGC 
TACAAGACTC AAGTTCAGCC TCTTACGCAC TTCTATGATG ACTACGGCCT AAAGGCCGAC 
GAAGATGAGG AGGAAGAAGA GGAGGAAGAG GAGGATGAAG ATGATGACCA TCACCACCAT 
CACGGCTGCG GAAGCGAGTG CGAGAGCGAG TGCGGAAGTG AGTGTGACTC GGACGAGGAT 
ATGTCGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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NCU11027 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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