cot-3 cDNA ORF clone, Neurospora crassa OR74A

The following cot-3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cot-3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf03921 XM_957193.3
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Neurospora crassa OR74A translation elongation factor aEF-2 (cot-3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf03921
    Clone ID Related Accession (Same CDS sequence) XM_957193.3
    Accession Version XM_957193.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product translation elongation factor aEF-2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026504). On Feb 23, 2015 this sequence version replaced XM_957193.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGTCAACT TCACGATTGA CGAGATCCGT GCCTTGATGG ACAAGCCCAC TAACGTGCGC 
    AATATGTCCG TCATTGCGCA CGTTGATCAC GGCAAGTCCA CTTTGACCGA CTCTCTCCTC
    GCCAAGGCCG GTATCATTTC CTCCGGCAAG GCTGGTGAGG CTCGCGCCAC GGATACTCGT
    GCCGACGAGC AGGAGCGTGG TATCACCATC AAGTCCACTG CCATCTCCCT CTACGGCACC
    CTCCCCGATG AGGAGGACAT CAAGGACATC GTTGGCCAGA AGACTGACGG CAAGGATTTC
    TTGATCAACT TGATCGACTC GCCCGGTCAC GTTGATTTCT CTTCCGAAGT CACTGCTGCC
    CTCCGTGTCA CCGATGGTGC TCTCGTTGTC GTCGACACCG TCGAGGGTGT CTGCGTCCAG
    ACCGAGACCG TCCTTCGTCA GGCTCTCGGT GAGCGCATCA AGCCCGTTGT TGTCATCAAC
    AAGGTCGATC GTGCTCTTCT CGAGCTCCAG GTCTCCAAGG AGGACCTTTA CCAGTCCTTC
    TCTCGTACCA TCGAGTCCGT TAACGTCATC ATCTCTACCT ACTTCGACAA GTCCCTTGGT
    GACGTCCAGG TCTACCCCGA CCGCGGTACC GTTGCCTTCG GTTCCGGTCT CCACGGCTGG
    GCCTTCACCA TCCGCCAGTT CGCCACCCGC TACGCCAAGA AGTTCGGTGT CGACCGCAAC
    AAGATGATGG AGCGTCTCTG GGGCGACAAC TACTTCAACC CCAAGACCAA GAAGTGGACC
    AAGAACGGCA CCTATGAGGG CAAGGAGCTC GAGCGTGCCT TCAACCAGTT CATTCTCGAC
    CCCATCTTCA AGATCTTCTC CGCTGTCATG AACTTCAAGA AGGACGAGGT TGCCGCTCTC
    CTCGAGAAGC TCAACCTTAA GCTCGCTACT GACGACAGAG AGAAGGAGGG CAAGCAGCTT
    CTCAAGGCCG TCATGAAGGC CTTCCTTCCC GCTGCTGACT GCTTGTTGGA GATGATGATC
    CTCCACCTTC CTTCCCCCGT CACCGCCCAG GCTTACCGTG CCGAGACTCT CTACGAGGGT
    CCCCAGGACG ACGAGGCCGC CATGGCCATC AAGACCTGCG ACCCCAAGGG TCCTCTCATG
    CTCTACGTCT CCAAGATGGT TCCCACTTCC GATAAGGGTC GTTTCTACGC CTTCGGTCGT
    GTCTTCGCCG GTACCGTCCG CTCCGGTCTC AAGGTCCGTA TCCAGGGCCC CAACTACACC
    CCCGGCAAGA AGGAGGATCT CTTCATCAAG GCCATTCAGC GTACCGTCCT CATGATGGGT
    GGCAAGGTCG AGCCCATCGA CGACATGCCC GCTGGTAACA TTGTTGGTCT CGTCGGTATC
    GACCAGTTCC TTCTCAAGTC TGGTACCCTT ACCACCTCCG AGACTGCCCA CAACATGAAG
    GTCATGAAGT TCTCCGTCTC TCCCGTCGTC CAGCGTTCCG TCCAGGTCAA GAACGCCCAG
    GATCTCCCCA AGCTTGTCGA GGGTCTCAAG CGTCTCTCCA AGTCCGACCC TTGCGTCCTT
    ACCTTCTCCA ACGAGTCTGG TGAGCACGTC GTTGCTGGTG CTGGTGAGCT CCATCTTGAG
    ATTTGCTTGA ACGATCTTGA GAACGACCAC GCCGGTGTCC CTCTTACCAT CTCCGACCCC
    GTCGTCCAGT ACCGTGAGAC CGTCGCTGGC AAGTCCTCCA TGACTGCCCT CTCCAAGTCT
    CCCAACAAGC ACAACCGTCT CTACATGGTT GCCGAGCCCC TTGAGGAGGA CCTCTGCCTT
    GCCATCGAGG CCGGCAAGAT CACCCCCCGT GACGATTTCA AGGCCCGTGC CCGTATCCTC
    GCTGATGACT TCGGCTGGGA CGTCACTGAT GCTCGTAAGA TCTGGGCTTT CGGCCCCGAC
    ACCAACGGTG CCAACTTGCT CGTCGACCAG ACCAAGGCCG TTCAGTACCT CAACGAAATC
    AAGGATTCCG TTGTCTCCGG TTTCCAGTGG GCCACCCGTG AGGGTCCCAT TGGTGAGGAG
    CCCATGCGCT CCATCCGCTT CAACATCCTT GATGTTACCC TCCACGCTGA TGCTATTCAC
    CGTGGTGGCG GTCAGATCAT CCCTACTGCT CGTCGCGTTC TTTACGCTGC TACCCTTCTT
    GCCGAGCCCT CGCTCCTCGA GCCCGTGTTC TTGGTCGAGA TCCAGGTTCC CGAGCAGGCC
    ATGGGTGGTG TCTACGGTGT CCTCACCCGC CGTCGTGGTC ACGTCTTCGG CGAGGAGCAG
    CGCCCCGGCA CTCCCCTCTT CACCATCAAG GCCTATCTCC CCGTCATGGA GTCTTTCGGC
    TTCAACGGCG ATCTTCGTGC CGCCACCTCC GGCCAGGCTT TCCCCCAGTC CGTCTTCGAT
    CACTGGGAGC GTCTCCCCGG TGGCTCTCCT CTCGACTCCA CCTCCAAGGT TGGTCAGATC
    GTCCAGGAGA TGCGCAAGCG CAAGGGTCTC AAGGTCGAGG TTCCCGGCTA CGAGAACTAC
    TACGACAAGC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_962286.2
    CDS414..2948
    Translation

    Target ORF information:

    RefSeq Version XM_957193.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A translation elongation factor aEF-2 (cot-3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_957193.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGGTCAACT TCACGATTGA CGAGATCCGT GCCTTGATGG ACAAGCCCAC TAACGTGCGC 
    AATATGTCCG TCATTGCGCA CGTTGATCAC GGCAAGTCCA CTTTGACCGA CTCTCTCCTC
    GCCAAGGCCG GTATCATTTC CTCCGGCAAG GCTGGTGAGG CTCGCGCCAC GGATACTCGT
    GCCGACGAGC AGGAGCGTGG TATCACCATC AAGTCCACTG CCATCTCCCT CTACGGCACC
    CTCCCCGATG AGGAGGACAT CAAGGACATC GTTGGCCAGA AGACTGACGG CAAGGATTTC
    TTGATCAACT TGATCGACTC GCCCGGTCAC GTTGATTTCT CTTCCGAAGT CACTGCTGCC
    CTCCGTGTCA CCGATGGTGC TCTCGTTGTC GTCGACACCG TCGAGGGTGT CTGCGTCCAG
    ACCGAGACCG TCCTTCGTCA GGCTCTCGGT GAGCGCATCA AGCCCGTTGT TGTCATCAAC
    AAGGTCGATC GTGCTCTTCT CGAGCTCCAG GTCTCCAAGG AGGACCTTTA CCAGTCCTTC
    TCTCGTACCA TCGAGTCCGT TAACGTCATC ATCTCTACCT ACTTCGACAA GTCCCTTGGT
    GACGTCCAGG TCTACCCCGA CCGCGGTACC GTTGCCTTCG GTTCCGGTCT CCACGGCTGG
    GCCTTCACCA TCCGCCAGTT CGCCACCCGC TACGCCAAGA AGTTCGGTGT CGACCGCAAC
    AAGATGATGG AGCGTCTCTG GGGCGACAAC TACTTCAACC CCAAGACCAA GAAGTGGACC
    AAGAACGGCA CCTATGAGGG CAAGGAGCTC GAGCGTGCCT TCAACCAGTT CATTCTCGAC
    CCCATCTTCA AGATCTTCTC CGCTGTCATG AACTTCAAGA AGGACGAGGT TGCCGCTCTC
    CTCGAGAAGC TCAACCTTAA GCTCGCTACT GACGACAGAG AGAAGGAGGG CAAGCAGCTT
    CTCAAGGCCG TCATGAAGGC CTTCCTTCCC GCTGCTGACT GCTTGTTGGA GATGATGATC
    CTCCACCTTC CTTCCCCCGT CACCGCCCAG GCTTACCGTG CCGAGACTCT CTACGAGGGT
    CCCCAGGACG ACGAGGCCGC CATGGCCATC AAGACCTGCG ACCCCAAGGG TCCTCTCATG
    CTCTACGTCT CCAAGATGGT TCCCACTTCC GATAAGGGTC GTTTCTACGC CTTCGGTCGT
    GTCTTCGCCG GTACCGTCCG CTCCGGTCTC AAGGTCCGTA TCCAGGGCCC CAACTACACC
    CCCGGCAAGA AGGAGGATCT CTTCATCAAG GCCATTCAGC GTACCGTCCT CATGATGGGT
    GGCAAGGTCG AGCCCATCGA CGACATGCCC GCTGGTAACA TTGTTGGTCT CGTCGGTATC
    GACCAGTTCC TTCTCAAGTC TGGTACCCTT ACCACCTCCG AGACTGCCCA CAACATGAAG
    GTCATGAAGT TCTCCGTCTC TCCCGTCGTC CAGCGTTCCG TCCAGGTCAA GAACGCCCAG
    GATCTCCCCA AGCTTGTCGA GGGTCTCAAG CGTCTCTCCA AGTCCGACCC TTGCGTCCTT
    ACCTTCTCCA ACGAGTCTGG TGAGCACGTC GTTGCTGGTG CTGGTGAGCT CCATCTTGAG
    ATTTGCTTGA ACGATCTTGA GAACGACCAC GCCGGTGTCC CTCTTACCAT CTCCGACCCC
    GTCGTCCAGT ACCGTGAGAC CGTCGCTGGC AAGTCCTCCA TGACTGCCCT CTCCAAGTCT
    CCCAACAAGC ACAACCGTCT CTACATGGTT GCCGAGCCCC TTGAGGAGGA CCTCTGCCTT
    GCCATCGAGG CCGGCAAGAT CACCCCCCGT GACGATTTCA AGGCCCGTGC CCGTATCCTC
    GCTGATGACT TCGGCTGGGA CGTCACTGAT GCTCGTAAGA TCTGGGCTTT CGGCCCCGAC
    ACCAACGGTG CCAACTTGCT CGTCGACCAG ACCAAGGCCG TTCAGTACCT CAACGAAATC
    AAGGATTCCG TTGTCTCCGG TTTCCAGTGG GCCACCCGTG AGGGTCCCAT TGGTGAGGAG
    CCCATGCGCT CCATCCGCTT CAACATCCTT GATGTTACCC TCCACGCTGA TGCTATTCAC
    CGTGGTGGCG GTCAGATCAT CCCTACTGCT CGTCGCGTTC TTTACGCTGC TACCCTTCTT
    GCCGAGCCCT CGCTCCTCGA GCCCGTGTTC TTGGTCGAGA TCCAGGTTCC CGAGCAGGCC
    ATGGGTGGTG TCTACGGTGT CCTCACCCGC CGTCGTGGTC ACGTCTTCGG CGAGGAGCAG
    CGCCCCGGCA CTCCCCTCTT CACCATCAAG GCCTATCTCC CCGTCATGGA GTCTTTCGGC
    TTCAACGGCG ATCTTCGTGC CGCCACCTCC GGCCAGGCTT TCCCCCAGTC CGTCTTCGAT
    CACTGGGAGC GTCTCCCCGG TGGCTCTCCT CTCGACTCCA CCTCCAAGGT TGGTCAGATC
    GTCCAGGAGA TGCGCAAGCG CAAGGGTCTC AAGGTCGAGG TTCCCGGCTA CGAGAACTAC
    TACGACAAGC TCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B