NCU08225 cDNA ORF clone, Neurospora crassa OR74A

The following NCU08225 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCU08225 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf07068 XM_954222.3
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Neurospora crassa OR74A high affinity nickel transporter nic1 (NCU08225), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf07068
    Clone ID Related Accession (Same CDS sequence) XM_954222.3
    Accession Version XM_954222.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product high affinity nickel transporter nic1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026503). On Feb 23, 2015 this sequence version replaced XM_954222.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCCCGCC TCAAACTCCC CCACCTCCGG CTCCCACCAC CCCTCTCCTA CATCCCCCGC 
    CCCGCCCTCC ACCTCATCAC CCTCCTCATC CTCATCAACT GCCTCGTCTG GGCCGGCGTC
    GGCATCACCC TGCACTACTT CCCCAAGATG ATCTCGCCCG CCGTGCTCTC CTACACCCTC
    GGCCTCCGCC ACGCCCTCGA CGCCGACCAC ATCAGCGCCA TCGACCTGAT GACGCGCCGT
    CTGATTGCTT CCGGTCAGCG ACCCGTAGCC GTGGGCACCT TTTTCAGCTT GGGACACAGC
    ACCGTTGTCA TCATCACTTG CATCGTGGTA GCTGCGACGA GTGGAGCGCT AAGAGACAGG
    TTTGATGGGT TTCAGCGGGT GGGCGGCATC ATTGGCACGT CTGTGAGCGC CGCCTTTTTG
    ATTATCTTGG GAGTGGGGAA CGGGTGGGTG TTGTATAAGC TGGTGAAGCG GTTGCGGGTG
    GTGATGAAGG AGCAGAGGGA GCGCAGAAGG AGGGTGGAGG AAGGAGAGGA TTTGCGAGCC
    GAGGAGGAGC AGGAGGCCAT GAATAACTTG CAGTTGGAAG GGGCCGGGTT TCTGGCGAGG
    GTGTTTAGGA GGGTGTTTGC CATTGTGGAT CGGCCGTGGA AGATGTACCC GCTGGGTGTA
    CTGTTTGGAC TCGGGTTCGA TACCAGTTCG GAGGTGGCCT TGCTGGGGAT TGCGAGCATA
    CAGGCAGTGC AGGGGACGAG CATCTGGTTG ATTCTGATTT TTCCGATTTT GTTTACTGCC
    GGCATGTGCA TGCTCGACAC GACCGACGGC GCCCTGATGA TGGCCCTGTA CACATCCAAG
    GCCTTCTCGA GAGACGTGGT GGCCATCCTG TACTACTCGA TTGTGCTTAC CGGCATCACG
    GTCGTTGTGT CGGCCTTCAT CGGCGTCATC CAGATCCTGT CGCTTGCCTA CAACGTGGCC
    GAGCCGACAG GTCGTTTCTG GGATGGGGTT GAAGCGATAG GCGATCACTA CGACGTTATT
    GGCGGATGCA TCTGCGGCTT GTTTGTCGTT GTCGGTCTGG CGTCGGTCAT CATCTATCGC
    CCCTGGAGGA AAAAGATGGA GGATCGGATG GATGCCATGA GCATATTGGA TGTCGAAAGC
    CCGGTCGCGT CGCCCGCTGC GGTTGCGGAT GGTGCTATGA GCCCGTATGC CGATAGTCCG
    GTGGTCACGC CCTTGGAACG TGTTCCGGAA CGGATTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_959315.1
    CDS374..1615
    Translation

    Target ORF information:

    RefSeq Version XM_954222.3
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A high affinity nickel transporter nic1 (NCU08225), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_954222.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCCCGCC TCAAACTCCC CCACCTCCGG CTCCCACCAC CCCTCTCCTA CATCCCCCGC 
    CCCGCCCTCC ACCTCATCAC CCTCCTCATC CTCATCAACT GCCTCGTCTG GGCCGGCGTC
    GGCATCACCC TGCACTACTT CCCCAAGATG ATCTCGCCCG CCGTGCTCTC CTACACCCTC
    GGCCTCCGCC ACGCCCTCGA CGCCGACCAC ATCAGCGCCA TCGACCTGAT GACGCGCCGT
    CTGATTGCTT CCGGTCAGCG ACCCGTAGCC GTGGGCACCT TTTTCAGCTT GGGACACAGC
    ACCGTTGTCA TCATCACTTG CATCGTGGTA GCTGCGACGA GTGGAGCGCT AAGAGACAGG
    TTTGATGGGT TTCAGCGGGT GGGCGGCATC ATTGGCACGT CTGTGAGCGC CGCCTTTTTG
    ATTATCTTGG GAGTGGGGAA CGGGTGGGTG TTGTATAAGC TGGTGAAGCG GTTGCGGGTG
    GTGATGAAGG AGCAGAGGGA GCGCAGAAGG AGGGTGGAGG AAGGAGAGGA TTTGCGAGCC
    GAGGAGGAGC AGGAGGCCAT GAATAACTTG CAGTTGGAAG GGGCCGGGTT TCTGGCGAGG
    GTGTTTAGGA GGGTGTTTGC CATTGTGGAT CGGCCGTGGA AGATGTACCC GCTGGGTGTA
    CTGTTTGGAC TCGGGTTCGA TACCAGTTCG GAGGTGGCCT TGCTGGGGAT TGCGAGCATA
    CAGGCAGTGC AGGGGACGAG CATCTGGTTG ATTCTGATTT TTCCGATTTT GTTTACTGCC
    GGCATGTGCA TGCTCGACAC GACCGACGGC GCCCTGATGA TGGCCCTGTA CACATCCAAG
    GCCTTCTCGA GAGACGTGGT GGCCATCCTG TACTACTCGA TTGTGCTTAC CGGCATCACG
    GTCGTTGTGT CGGCCTTCAT CGGCGTCATC CAGATCCTGT CGCTTGCCTA CAACGTGGCC
    GAGCCGACAG GTCGTTTCTG GGATGGGGTT GAAGCGATAG GCGATCACTA CGACGTTATT
    GGCGGATGCA TCTGCGGCTT GTTTGTCGTT GTCGGTCTGG CGTCGGTCAT CATCTATCGC
    CCCTGGAGGA AAAAGATGGA GGATCGGATG GATGCCATGA GCATATTGGA TGTCGAAAGC
    CCGGTCGCGT CGCCCGCTGC GGTTGCGGAT GGTGCTATGA GCCCGTATGC CGATAGTCCG
    GTGGTCACGC CCTTGGAACG TGTTCCGGAA CGGATTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
    Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B