crf2-1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	crf2-1
Entrez Gene ID	3874826
Full Name	chromatin remodelling factor 2-1
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011396443.1	XP_011394745.1	SNF2 family helicase/ATPase, variant
XM_011396444.1	XP_011394746.1	SNF2 family helicase/ATPase

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_06776	XP_003709493.1
Neurospora crassa	NCU08919	XP_958688.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following crf2-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crf2-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf10805	XM_011396443.1 Latest version!	Neurospora crassa OR74A SNF2 family helicase/ATPase, variant (crf2-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ONf10806	XM_011396444.1 Latest version!	Neurospora crassa OR74A SNF2 family helicase/ATPase (crf2-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crf2-1 ( XM_011396443.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394745.1 Neurospora crassa OR74A SNF2 family helicase/ATPase, variant (crf2-1), mRNA.

Price & Availability↑

CloneID	ONf10805
Clone ID Related Accession (Same CDS sequence)	XM_011396443.1
Accession Version	XM_011396443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	SNF2 family helicase/ATPase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGGACCACT TCTCGACAGT GCTGCAGCGA CCTCCTCATT TTGACGAGGA TGGAACAGAA 
GGGAGAGGAG ATCGAGGAAA CGGCGCCGGT CCAGGTCCAG CACCACCACC ACCACCACCA 
CGCGGAGGTC TGCGCGACAT TCTCAACCCA GTGAGCAGCA ACAGCGCCGT ACAATCCCAA 
GCAGCAGCAG CAGCACCACC ACCACCACCA GCAGTAGCGT CATCATCGCT CCATGGAATT 
GCAGCATCAG TGCCGCCGCC CTCGTCGACA AATTCCATGC GCGCGACGCC GCATAGCTCG 
TCCTCGTTCA ATTTACGATC GCCGACGCGG GAACCGTCGG AATACCGTCA TCCACTTTCC 
TCTCTGGCGA CTCCAGCACC CTTTGCGGCC TCGCCTCCCA CATCCATTGC CAACGCCAAC 
AACACCAACA ACAACAACGC GCTAGGTGCC GCCGGCAGTC TGAGCAGTCA ACCACCACCA 
CCACCACCCA CGGGTCCGCG CTCAATTCTT AATCCTCCAA CACCAAGCCA ACAACATCAA 
CAACAACACC ACCATCCCAA TCCCTTTGTC GCAGCATCCG CACCCTCGCT GCCGCCGCCG 
CCGTCCTCGT TGCAAGCTCC ACCTGCCATT ACACCCATCG CCGGCCTATC AGCTCCGGCC 
CCGGCATCGG GTTCGCTGCC TCTGTCAGCA GGAGGCATCG GAAACTCCAT CACGGTCTCG 
TCCAGCTCCC AACCGCCAGC CCGAGCATCG CAACTGCACG CGCCGTCGGC CTACTATTCG 
CCGGCAGAAT CGTTCCGCGA TCGGGATTCG TCAGTACGCG AAAAATCATC GACAGGCGGC 
AGCTTTTACG ACCCAACCGC CGAGGCCAGC AACGGCATTA GTGGCTCGAG TCCACGAAAA 
GATCGAGATC GCGACCGTGA TCACCGGGGT ACAACAAGGG AATCACAGCG CCGCAGTGTT 
TCGGGCCATT CCGACACGGG CTCCTCCTGG CGAAACGCGA CCCAGACTTC CGCCAGCAAC 
AAGACCCGCG ATCCGTACAA CTATTCCCCG TCCTCGGCCG ATTATTATAA CACCAGGAAA 
AAGGAAAACT ACCCGGTCGA TAATACGACG TCTTCATCGA TTGCAGCTCC GTCCAACTTT 
ACTGTTGCGA CGCGTAGTCC TGTAGCCGCG CTTTCCCATC CGGCGTCCAT AGCTGCACCA 
GCTAGTGTCG GCAGTCTCAC CGGATCCATC AGCCCCAGGC TTTCACTCAG ACCACCAAGC 
ATGGCCTCTC CCACGATACG AAGCGCCGTC TTAGCTAATC CCACAAACGG CACAACTTCC 
ACCGCACTAC CGGCTCTAGG CAGGAACGAT TCGCCACCTT CCAAGATGTC TCCCGGTACA 
TCAACCAACC CTAGCCGTGC CGCTGGTGTC ATGTCCTTTT CCAATATTCT CTCGAGCTCC 
GAGCCCGTTC CTAGACCCCG TGCGACTTCA CCCAACAACC CGGATGATGA CGACGACGTT 
CCCATGAAGG TCGAGCGCGC CGACTCATCC GAGAAGGTCG TCAAGGAGAA GAAGGAGAGG 
AAACCGCGCC AGCCCAAGCA ACCACGCATC TCTGACATCA GGCACTCCGA GTCCACCCCC 
AAAGGGCGAC GAGGGTCGAC CAAGCAAGAG AGTCCACTGC CCAACATTCG CATTCCGGCC 
AAGAGAATGG CCAACGGTGC TCCCAAGCAA CAAAAGACAT TCTCGGCGGA GAATGAGGAG 
AAGATCAGGA AAGCGATGGA TCGAATCGAA ACACGAGAGC TTCCCCACGA GGACGAATTC 
GAAGAGGAGT TGCGCTTGTG GAGAGAGCGA CGCGAGTACA AGAGGCAACA GATGAACCAA 
CGCGACCTTC GCCAGCGTCG ACAGAGGAGA GCCGACTACA CCGAAGTCGA AGCCCAGAAG 
CTCAAGCTTC ATGCCGATTT TGGCAAGCGT CGATATGACG ACCTTAATTA CGATGATGCG 
TTGCAGGAGG TTCGGGAGCG CGAGCTCTTT GCCGAGAAGG AGCGCAAGAA GGACATGCAG 
CGTAAGAGGC GGCGCGAGAA GTCCATGGCC ACTACCATGG AGGCAAAGGC AGCCGCGTTG 
GCCAGGGCCT CTGCAGCGCA GGATGAAGCC GAAAGACAGA AATACATGCG CGAAGCAGAA 
CGGGCGAACA AGAAGGTTCA ACAGACCCGC CTCATTTTGC AGAAGGGCAT CAAGGGCCCG 
TCGCGGAACA CCGGTCCCAT TGAGCCGAAC CTGGAAGGCG GTACTATGGC TACGTTCCAG 
GCCGAGAACA TGGAGCCTGG TAAGACGAAG GGTAAGGGTC GTGCCGGCGC GAGACCCAAG 
AAATCCAAGG AGCAGAAGCA GGCCGAGAAG GATGCCGCCG AAGCTGCCCA AGCCGCTCTT 
GATGCCGGTC TCGAGCTGCC GCCCAAGGAA GAAACCAACA AGATCCGCAT CAAGCTTACC 
AAGACTAAGG CGCCCAAGGA GGCTGATGTG GACAAGGATA AGGAGAACAA GGAGCCTCAA 
GAGCCGAAGG AGCCTAAGGA GCCCAAGGAG AAGGTGATAA AGGAAAAGGT TGTGGAGGAA 
CCCAAGGACC CGTTGGAACT CAAGTTCCAG TCCAAGGGCT TCAACCAGAT CTATGACCAG 
ATCTGGCGCG ATCTTGCTAG GAAGGACGTC AACAAGGTGT TCAGATTGGC GATCGATTCG 
TATTCTACCA AGTCCTCTAA CCTCAAGAAG ACTGCCATCC TCGCGTCCAA AGAGGCCAAG 
AGATGGCAAC TCCGTACCAA CAAGGGCACC AAGGATTTGC AAGCTCGCGC CAAGCGTGTC 
ATGCGTGACA TGATGGGCTT CTGGAAGCGC AACGAGCGTG AAGAGCGTGA TCTCCGGAAG 
GCAGCTGAAA AGCAAGAGCT GGAGAACGCT CGCAAGGAGG AGGCAGACCG CGAAGCCGCT 
CGCCAGAAGA GGAAGCTCAA CTTCCTCATC TCGCAGACCG AACTGTACTC GCATTTCATT 
GGCAAGAAGA TCAAGACCAA CGAGGTGGAG CGCAGCACGG ATCACCCCGA TGAGATCGCG 
GCTGAGAAGG ACAAGATCCC GGAGAACGAG ATGGACATCG AGGTGCCTAC TGGCCCCATT 
GGCGCCAAGG TCACCAACTT TGAGAATCTG GACTTTGACG CCGAAGATGA ATCGACGCTG 
CGGGCTGCTG CCATGGCGAA TGCGCAGAAT GCCATTGCCG AGGCCCAGAA GAAGGCTAGG 
GAGTTCAACA AGGAAGAGTC CAAGCTTGAT GAGGATGGCG AGATGAACTT CCAGAACCCA 
ACCATGATGG GCGACGTCGA GATCGAGCAG CCCAAGCTTC TCAACTGCCA GCTCAAGGAG 
TACCAGCTCA AGGGTCTCAA TTGGCTCGTC AACCTGTACG AGCAAGGCAT AAACGGTATC 
CTGGCCGACG AAATGGGTCT AGGAAAGACG GTGCAGTCCA TTTCCGTCAT GGCCTACCTT 
GCCGAGAAGT ACGATATCTG GGGTCCCTTC CTGGTTGTTG CGCCTGCATC GACCCTACAC 
AACTGGCAGC AGGAAATCAC AAAGTTCGTT CCCCAGTTCA AGGTGCTCCC TTACTGGGGT 
ACTGCAGGCG ACCGCAAGGT GCTGCGTAAG TTCTGGGATC GCAAGCACAC GACCTACAAG 
AAGGACGCCC CCTTTCATGT CATGATTACC TCGTACCAAC TGGTCGTCTC TGATGTTGCG 
TACTTCCAAA AGATGAAGTG GCAGTACATG ATCCTGGATG AAGCCCAGGC CATCAAGAGT 
TCACAGAGTT CGCGTTGGAA GTGCCTGTTG GGCTTCCACT GCCGTAACCG TCTCCTGTTG 
ACGGGTACGC CTATCCAGAA CAACATGCAG GAACTCTGGG CCCTGCTGCA TTTCATCATG 
CCGTCACTGT TCGATTCTCA CGATGAGTTT AGTGAATGGT TCTCCAAGGA TATCGAGTCT 
CATGCTCAGA GTAATACCAA GCTCAACGAG GACCAGCTGA AGCGTCTGCA CATGATCCTC 
AAACCCTTTA TGCTGCGTCG TGTCAAGAAG CATGTCCAAA AGGAACTCGG CGACAAGATC 
GAGATGGACG TTTTCTGCGA TCTCACATAC AGACAGCGTG CCATGTACGC CAATTTGCGC 
AACCAGATCA GCATCATGGA CCTCATCGAG AAGGCGACAC TGGGCGACGA CGATTCTGCT 
AGTCTCATGA ATCTCGTCAT GCAGTTCCGT AAGGTGTGCA ACCACCCTGA CCTCTTCGAG 
CGGGCGGATA CCGCTTCGCC GTACTCGTTC GGCCACTTTG CCGAGACAGC CTCGTTCATC 
CGTGAGGGTA GCCAGGTTAC CGTCGGTTAC TCTACGCGCA GCTTGATCCA GTATGAGCTG 
CCTAGACTGC TCTGGCGCGA CGGAGGACGG CTGCACAAGG CTGGTGAAGA CAACCAGGTC 
GCTGGATGGC GGAACCAGTG GCTCAATGAG AAGTTTAACA TCTGGACTCC GGAGCATATC 
CGTGAGAGCC TAGCCGGGAC TGACAACTTC TCGTGGTTGC GCTTTGCCGA CACGAGCTAC 
GAGGAGGCCT ACCGGGCTAG CCATAAGGAT CTCTTTGCTC GGGCTGTTGA GATGTCCACC 
AAGAAGAACC GGCTCGCGGA GATCAAGATC GCTTACGATG AGCCGGAGGA CTTGAACTTC 
ACTCCGGCGC ATGCCCTCTT CCACATCAGG GAACGTGAGG ATCGGAGGCC GTTGGCTGAG 
ATCACTGAGC AAGGCATTCT GGGCAGTTTG ATGAATGTCT CCCGGAGTGC ATTCTCCGAG 
ACTGGCCTCG GTCGTCTCGA GCAGGCTGCG GCTCCCAAGG CCTCGGCTCC TCCTATCGAG 
GTGGTCTGCG ACAGCCGTAG CGCAGTGGTT GAGCGGGAGA ACATCATGTT CAACGCTCCC 
ATGCGCAAGG TCCTCTTTGG ACCTACCCTG GCCGAGGAGA AGGCGCTCGT CGTACAAAAG 
GTGCCGCCAT CACGCTACCC ACCGCCAGCG TTGCTTCCTG CACCAGACAA GGAGAAGCAG 
AAGTTCACCA ACATCACCGT CCCGTCGATG CGCCGCTTTG TCACCGACTC GGGCAAGCTC 
GCCAAGCTCG ACGAGCTCCT GCGCGAGCTC AAGGAGAATG GCCACCGCGT CCTCCTCTAC 
TTCCAAATGA CGCGCATGAT CGATCTCATG GAAGAGTATC TTACTTACCG CAACTACAAG 
TACTGCCGAC TCGACGGTTC AACCAAGCTC GAAGACCGTC GCGACACCGT TGCCGACTTC 
CAGACCCGCC CCGAGATCTT CATCTTCCTT TTGTCTACTC GTGCCGGTGG TCTGGGCATC 
AACCTGACCT CTGCCGACAC CGTCATCTTC TACGACTCGG ACTGGAACCC GACCATCGAT 
TCGCAAGCCA TGGATCGTGC CCATCGTCTG GGTCAGACCA AACAGGTTAC TGTCTACCGT 
CTTATCACCA GGGGCACGAT CGAGGAACGC ATCCGCAAGC GTGCCATGCA AAAGGAAGAA 
GTCCAGCGCG TGGTTATCAC GGGTGGCTCA TCGGCTGCTG GTGGCGGTGT GGACTTCTCT 
GGTCGTCGTG CGCCTGAGAA CAGGAACCGT GACATTGCCA TGTGGCTGGC GGATGACGAG 
CAGGCGGAGA TGATTGAGAA GAGGGAGCGC GAGCTGTTGG AGAGTGGAGA GTTGGATAAG 
ATGCAGAAGA AGAGTAGGGG AGGTAACAAG AGGAAGAGAG GAGGTGCTGG GGGAGAAGGG 
AAGGAGGTTA GTTTGGATGA GATGTATCAT GAGGGTGAGG GCAACTTTGA TGATGGAGGA 
AATAATATCA AGGGTAGCGG AACGGCTACG CCTAATGGTG CTGCCGGTGG TGAGGGAGGT 
GATGGGAAGG GAGCAGTGGG AGGAGCGGCG AAGAAGAGGA AGACGGGTGG AAGCAAGAAG 
GCCAAGACGA CCAAGCAGAG ATTGGCTATT GCTGACGGGG AGATTGATAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394745.1
CDS	987..6980
Translation	MDHFSTVLQRPPHFDEDGTEGRGDRGNGAGPGPAPPPPPPRGGLRDILNPVSSNSAVQSQAAAAAPPPPPAVASSSLHGIAASVPPPSSTNSMRATPHSSSSFNLRSPTREPSEYRHPLSSLATPAPFAASPPTSIANANNTNNNNALGAAGSLSSQPPPPPPTGPRSILNPPTPSQQHQQQHHHPNPFVAASAPSLPPPPSSLQAPPAITPIAGLSAPAPASGSLPLSAGGIGNSITVSSSSQPPARASQLHAPSAYYSPAESFRDRDSSVREKSSTGGSFYDPTAEASNGISGSSPRKDRDRDRDHRGTTRESQRRSVSGHSDTGSSWRNATQTSASNKTRDPYNYSPSSADYYNTRKKENYPVDNTTSSSIAAPSNFTVATRSPVAALSHPASIAAPASVGSLTGSISPRLSLRPPSMASPTIRSAVLANPTNGTTSTALPALGRNDSPPSKMSPGTSTNPSRAAGVMSFSNILSSSEPVPRPRATSPNNPDDDDDVPMKVERADSSEKVVKEKKERKPRQPKQPRISDIRHSESTPKGRRGSTKQESPLPNIRIPAKRMANGAPKQQKTFSAENEEKIRKAMDRIETRELPHEDEFEEELRLWRERREYKRQQMNQRDLRQRRQRRADYTEVEAQKLKLHADFGKRRYDDLNYDDALQEVRERELFAEKERKKDMQRKRRREKSMATTMEAKAAALARASAAQDEAERQKYMREAERANKKVQQTRLILQKGIKGPSRNTGPIEPNLEGGTMATFQAENMEPGKTKGKGRAGARPKKSKEQKQAEKDAAEAAQAALDAGLELPPKEETNKIRIKLTKTKAPKEADVDKDKENKEPQEPKEPKEPKEKVIKEKVVEEPKDPLELKFQSKGFNQIYDQIWRDLARKDVNKVFRLAIDSYSTKSSNLKKTAILASKEAKRWQLRTNKGTKDLQARAKRVMRDMMGFWKRNEREERDLRKAAEKQELENARKEEADREAARQKRKLNFLISQTELYSHFIGKKIKTNEVERSTDHPDEIAAEKDKIPENEMDIEVPTGPIGAKVTNFENLDFDAEDESTLRAAAMANAQNAIAEAQKKAREFNKEESKLDEDGEMNFQNPTMMGDVEIEQPKLLNCQLKEYQLKGLNWLVNLYEQGINGILADEMGLGKTVQSISVMAYLAEKYDIWGPFLVVAPASTLHNWQQEITKFVPQFKVLPYWGTAGDRKVLRKFWDRKHTTYKKDAPFHVMITSYQLVVSDVAYFQKMKWQYMILDEAQAIKSSQSSRWKCLLGFHCRNRLLLTGTPIQNNMQELWALLHFIMPSLFDSHDEFSEWFSKDIESHAQSNTKLNEDQLKRLHMILKPFMLRRVKKHVQKELGDKIEMDVFCDLTYRQRAMYANLRNQISIMDLIEKATLGDDDSASLMNLVMQFRKVCNHPDLFERADTASPYSFGHFAETASFIREGSQVTVGYSTRSLIQYELPRLLWRDGGRLHKAGEDNQVAGWRNQWLNEKFNIWTPEHIRESLAGTDNFSWLRFADTSYEEAYRASHKDLFARAVEMSTKKNRLAEIKIAYDEPEDLNFTPAHALFHIREREDRRPLAEITEQGILGSLMNVSRSAFSETGLGRLEQAAAPKASAPPIEVVCDSRSAVVERENIMFNAPMRKVLFGPTLAEEKALVVQKVPPSRYPPPALLPAPDKEKQKFTNITVPSMRRFVTDSGKLAKLDELLRELKENGHRVLLYFQMTRMIDLMEEYLTYRNYKYCRLDGSTKLEDRRDTVADFQTRPEIFIFLLSTRAGGLGINLTSADTVIFYDSDWNPTIDSQAMDRAHRLGQTKQVTVYRLITRGTIEERIRKRAMQKEEVQRVVITGGSSAAGGGVDFSGRRAPENRNRDIAMWLADDEQAEMIEKRERELLESGELDKMQKKSRGGNKRKRGGAGGEGKEVSLDEMYHEGEGNFDDGGNNIKGSGTATPNGAAGGEGGDGKGAVGGAAKKRKTGGSKKAKTTKQRLAIADGEIDI

Target ORF information:

RefSeq Version	XM_011396443.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A SNF2 family helicase/ATPase, variant (crf2-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396443.1

ORF Insert Sequence:

ATGGACCACT TCTCGACAGT GCTGCAGCGA CCTCCTCATT TTGACGAGGA TGGAACAGAA 
GGGAGAGGAG ATCGAGGAAA CGGCGCCGGT CCAGGTCCAG CACCACCACC ACCACCACCA 
CGCGGAGGTC TGCGCGACAT TCTCAACCCA GTGAGCAGCA ACAGCGCCGT ACAATCCCAA 
GCAGCAGCAG CAGCACCACC ACCACCACCA GCAGTAGCGT CATCATCGCT CCATGGAATT 
GCAGCATCAG TGCCGCCGCC CTCGTCGACA AATTCCATGC GCGCGACGCC GCATAGCTCG 
TCCTCGTTCA ATTTACGATC GCCGACGCGG GAACCGTCGG AATACCGTCA TCCACTTTCC 
TCTCTGGCGA CTCCAGCACC CTTTGCGGCC TCGCCTCCCA CATCCATTGC CAACGCCAAC 
AACACCAACA ACAACAACGC GCTAGGTGCC GCCGGCAGTC TGAGCAGTCA ACCACCACCA 
CCACCACCCA CGGGTCCGCG CTCAATTCTT AATCCTCCAA CACCAAGCCA ACAACATCAA 
CAACAACACC ACCATCCCAA TCCCTTTGTC GCAGCATCCG CACCCTCGCT GCCGCCGCCG 
CCGTCCTCGT TGCAAGCTCC ACCTGCCATT ACACCCATCG CCGGCCTATC AGCTCCGGCC 
CCGGCATCGG GTTCGCTGCC TCTGTCAGCA GGAGGCATCG GAAACTCCAT CACGGTCTCG 
TCCAGCTCCC AACCGCCAGC CCGAGCATCG CAACTGCACG CGCCGTCGGC CTACTATTCG 
CCGGCAGAAT CGTTCCGCGA TCGGGATTCG TCAGTACGCG AAAAATCATC GACAGGCGGC 
AGCTTTTACG ACCCAACCGC CGAGGCCAGC AACGGCATTA GTGGCTCGAG TCCACGAAAA 
GATCGAGATC GCGACCGTGA TCACCGGGGT ACAACAAGGG AATCACAGCG CCGCAGTGTT 
TCGGGCCATT CCGACACGGG CTCCTCCTGG CGAAACGCGA CCCAGACTTC CGCCAGCAAC 
AAGACCCGCG ATCCGTACAA CTATTCCCCG TCCTCGGCCG ATTATTATAA CACCAGGAAA 
AAGGAAAACT ACCCGGTCGA TAATACGACG TCTTCATCGA TTGCAGCTCC GTCCAACTTT 
ACTGTTGCGA CGCGTAGTCC TGTAGCCGCG CTTTCCCATC CGGCGTCCAT AGCTGCACCA 
GCTAGTGTCG GCAGTCTCAC CGGATCCATC AGCCCCAGGC TTTCACTCAG ACCACCAAGC 
ATGGCCTCTC CCACGATACG AAGCGCCGTC TTAGCTAATC CCACAAACGG CACAACTTCC 
ACCGCACTAC CGGCTCTAGG CAGGAACGAT TCGCCACCTT CCAAGATGTC TCCCGGTACA 
TCAACCAACC CTAGCCGTGC CGCTGGTGTC ATGTCCTTTT CCAATATTCT CTCGAGCTCC 
GAGCCCGTTC CTAGACCCCG TGCGACTTCA CCCAACAACC CGGATGATGA CGACGACGTT 
CCCATGAAGG TCGAGCGCGC CGACTCATCC GAGAAGGTCG TCAAGGAGAA GAAGGAGAGG 
AAACCGCGCC AGCCCAAGCA ACCACGCATC TCTGACATCA GGCACTCCGA GTCCACCCCC 
AAAGGGCGAC GAGGGTCGAC CAAGCAAGAG AGTCCACTGC CCAACATTCG CATTCCGGCC 
AAGAGAATGG CCAACGGTGC TCCCAAGCAA CAAAAGACAT TCTCGGCGGA GAATGAGGAG 
AAGATCAGGA AAGCGATGGA TCGAATCGAA ACACGAGAGC TTCCCCACGA GGACGAATTC 
GAAGAGGAGT TGCGCTTGTG GAGAGAGCGA CGCGAGTACA AGAGGCAACA GATGAACCAA 
CGCGACCTTC GCCAGCGTCG ACAGAGGAGA GCCGACTACA CCGAAGTCGA AGCCCAGAAG 
CTCAAGCTTC ATGCCGATTT TGGCAAGCGT CGATATGACG ACCTTAATTA CGATGATGCG 
TTGCAGGAGG TTCGGGAGCG CGAGCTCTTT GCCGAGAAGG AGCGCAAGAA GGACATGCAG 
CGTAAGAGGC GGCGCGAGAA GTCCATGGCC ACTACCATGG AGGCAAAGGC AGCCGCGTTG 
GCCAGGGCCT CTGCAGCGCA GGATGAAGCC GAAAGACAGA AATACATGCG CGAAGCAGAA 
CGGGCGAACA AGAAGGTTCA ACAGACCCGC CTCATTTTGC AGAAGGGCAT CAAGGGCCCG 
TCGCGGAACA CCGGTCCCAT TGAGCCGAAC CTGGAAGGCG GTACTATGGC TACGTTCCAG 
GCCGAGAACA TGGAGCCTGG TAAGACGAAG GGTAAGGGTC GTGCCGGCGC GAGACCCAAG 
AAATCCAAGG AGCAGAAGCA GGCCGAGAAG GATGCCGCCG AAGCTGCCCA AGCCGCTCTT 
GATGCCGGTC TCGAGCTGCC GCCCAAGGAA GAAACCAACA AGATCCGCAT CAAGCTTACC 
AAGACTAAGG CGCCCAAGGA GGCTGATGTG GACAAGGATA AGGAGAACAA GGAGCCTCAA 
GAGCCGAAGG AGCCTAAGGA GCCCAAGGAG AAGGTGATAA AGGAAAAGGT TGTGGAGGAA 
CCCAAGGACC CGTTGGAACT CAAGTTCCAG TCCAAGGGCT TCAACCAGAT CTATGACCAG 
ATCTGGCGCG ATCTTGCTAG GAAGGACGTC AACAAGGTGT TCAGATTGGC GATCGATTCG 
TATTCTACCA AGTCCTCTAA CCTCAAGAAG ACTGCCATCC TCGCGTCCAA AGAGGCCAAG 
AGATGGCAAC TCCGTACCAA CAAGGGCACC AAGGATTTGC AAGCTCGCGC CAAGCGTGTC 
ATGCGTGACA TGATGGGCTT CTGGAAGCGC AACGAGCGTG AAGAGCGTGA TCTCCGGAAG 
GCAGCTGAAA AGCAAGAGCT GGAGAACGCT CGCAAGGAGG AGGCAGACCG CGAAGCCGCT 
CGCCAGAAGA GGAAGCTCAA CTTCCTCATC TCGCAGACCG AACTGTACTC GCATTTCATT 
GGCAAGAAGA TCAAGACCAA CGAGGTGGAG CGCAGCACGG ATCACCCCGA TGAGATCGCG 
GCTGAGAAGG ACAAGATCCC GGAGAACGAG ATGGACATCG AGGTGCCTAC TGGCCCCATT 
GGCGCCAAGG TCACCAACTT TGAGAATCTG GACTTTGACG CCGAAGATGA ATCGACGCTG 
CGGGCTGCTG CCATGGCGAA TGCGCAGAAT GCCATTGCCG AGGCCCAGAA GAAGGCTAGG 
GAGTTCAACA AGGAAGAGTC CAAGCTTGAT GAGGATGGCG AGATGAACTT CCAGAACCCA 
ACCATGATGG GCGACGTCGA GATCGAGCAG CCCAAGCTTC TCAACTGCCA GCTCAAGGAG 
TACCAGCTCA AGGGTCTCAA TTGGCTCGTC AACCTGTACG AGCAAGGCAT AAACGGTATC 
CTGGCCGACG AAATGGGTCT AGGAAAGACG GTGCAGTCCA TTTCCGTCAT GGCCTACCTT 
GCCGAGAAGT ACGATATCTG GGGTCCCTTC CTGGTTGTTG CGCCTGCATC GACCCTACAC 
AACTGGCAGC AGGAAATCAC AAAGTTCGTT CCCCAGTTCA AGGTGCTCCC TTACTGGGGT 
ACTGCAGGCG ACCGCAAGGT GCTGCGTAAG TTCTGGGATC GCAAGCACAC GACCTACAAG 
AAGGACGCCC CCTTTCATGT CATGATTACC TCGTACCAAC TGGTCGTCTC TGATGTTGCG 
TACTTCCAAA AGATGAAGTG GCAGTACATG ATCCTGGATG AAGCCCAGGC CATCAAGAGT 
TCACAGAGTT CGCGTTGGAA GTGCCTGTTG GGCTTCCACT GCCGTAACCG TCTCCTGTTG 
ACGGGTACGC CTATCCAGAA CAACATGCAG GAACTCTGGG CCCTGCTGCA TTTCATCATG 
CCGTCACTGT TCGATTCTCA CGATGAGTTT AGTGAATGGT TCTCCAAGGA TATCGAGTCT 
CATGCTCAGA GTAATACCAA GCTCAACGAG GACCAGCTGA AGCGTCTGCA CATGATCCTC 
AAACCCTTTA TGCTGCGTCG TGTCAAGAAG CATGTCCAAA AGGAACTCGG CGACAAGATC 
GAGATGGACG TTTTCTGCGA TCTCACATAC AGACAGCGTG CCATGTACGC CAATTTGCGC 
AACCAGATCA GCATCATGGA CCTCATCGAG AAGGCGACAC TGGGCGACGA CGATTCTGCT 
AGTCTCATGA ATCTCGTCAT GCAGTTCCGT AAGGTGTGCA ACCACCCTGA CCTCTTCGAG 
CGGGCGGATA CCGCTTCGCC GTACTCGTTC GGCCACTTTG CCGAGACAGC CTCGTTCATC 
CGTGAGGGTA GCCAGGTTAC CGTCGGTTAC TCTACGCGCA GCTTGATCCA GTATGAGCTG 
CCTAGACTGC TCTGGCGCGA CGGAGGACGG CTGCACAAGG CTGGTGAAGA CAACCAGGTC 
GCTGGATGGC GGAACCAGTG GCTCAATGAG AAGTTTAACA TCTGGACTCC GGAGCATATC 
CGTGAGAGCC TAGCCGGGAC TGACAACTTC TCGTGGTTGC GCTTTGCCGA CACGAGCTAC 
GAGGAGGCCT ACCGGGCTAG CCATAAGGAT CTCTTTGCTC GGGCTGTTGA GATGTCCACC 
AAGAAGAACC GGCTCGCGGA GATCAAGATC GCTTACGATG AGCCGGAGGA CTTGAACTTC 
ACTCCGGCGC ATGCCCTCTT CCACATCAGG GAACGTGAGG ATCGGAGGCC GTTGGCTGAG 
ATCACTGAGC AAGGCATTCT GGGCAGTTTG ATGAATGTCT CCCGGAGTGC ATTCTCCGAG 
ACTGGCCTCG GTCGTCTCGA GCAGGCTGCG GCTCCCAAGG CCTCGGCTCC TCCTATCGAG 
GTGGTCTGCG ACAGCCGTAG CGCAGTGGTT GAGCGGGAGA ACATCATGTT CAACGCTCCC 
ATGCGCAAGG TCCTCTTTGG ACCTACCCTG GCCGAGGAGA AGGCGCTCGT CGTACAAAAG 
GTGCCGCCAT CACGCTACCC ACCGCCAGCG TTGCTTCCTG CACCAGACAA GGAGAAGCAG 
AAGTTCACCA ACATCACCGT CCCGTCGATG CGCCGCTTTG TCACCGACTC GGGCAAGCTC 
GCCAAGCTCG ACGAGCTCCT GCGCGAGCTC AAGGAGAATG GCCACCGCGT CCTCCTCTAC 
TTCCAAATGA CGCGCATGAT CGATCTCATG GAAGAGTATC TTACTTACCG CAACTACAAG 
TACTGCCGAC TCGACGGTTC AACCAAGCTC GAAGACCGTC GCGACACCGT TGCCGACTTC 
CAGACCCGCC CCGAGATCTT CATCTTCCTT TTGTCTACTC GTGCCGGTGG TCTGGGCATC 
AACCTGACCT CTGCCGACAC CGTCATCTTC TACGACTCGG ACTGGAACCC GACCATCGAT 
TCGCAAGCCA TGGATCGTGC CCATCGTCTG GGTCAGACCA AACAGGTTAC TGTCTACCGT 
CTTATCACCA GGGGCACGAT CGAGGAACGC ATCCGCAAGC GTGCCATGCA AAAGGAAGAA 
GTCCAGCGCG TGGTTATCAC GGGTGGCTCA TCGGCTGCTG GTGGCGGTGT GGACTTCTCT 
GGTCGTCGTG CGCCTGAGAA CAGGAACCGT GACATTGCCA TGTGGCTGGC GGATGACGAG 
CAGGCGGAGA TGATTGAGAA GAGGGAGCGC GAGCTGTTGG AGAGTGGAGA GTTGGATAAG 
ATGCAGAAGA AGAGTAGGGG AGGTAACAAG AGGAAGAGAG GAGGTGCTGG GGGAGAAGGG 
AAGGAGGTTA GTTTGGATGA GATGTATCAT GAGGGTGAGG GCAACTTTGA TGATGGAGGA 
AATAATATCA AGGGTAGCGG AACGGCTACG CCTAATGGTG CTGCCGGTGG TGAGGGAGGT 
GATGGGAAGG GAGCAGTGGG AGGAGCGGCG AAGAAGAGGA AGACGGGTGG AAGCAAGAAG 
GCCAAGACGA CCAAGCAGAG ATTGGCTATT GCTGACGGGG AGATTGATAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crf2-1 ( XM_011396444.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011394746.1 Neurospora crassa OR74A SNF2 family helicase/ATPase (crf2-1), mRNA.

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CloneID	ONf10806
Clone ID Related Accession (Same CDS sequence)	XM_011396444.1
Accession Version	XM_011396444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	SNF2 family helicase/ATPase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505).

ATGGACCACT TCTCGACAGT GCTGCAGCGA CCTCCTCATT TTGACGAGGA TGGAACAGAA 
GGGAGAGGAG ATCGAGGAAA CGGCGCCGGT CCAGGTCCAG CACCACCACC ACCACCACCA 
CGCGGAGGTC TGCGCGACAT TCTCAACCCA GTGAGCAGCA ACAGCGCCGT ACAATCCCAA 
GCAGCAGCAG CAGCACCACC ACCACCACCA GCAGTAGCGT CATCATCGCT CCATGGAATT 
GCAGCATCAG TGCCGCCGCC CTCGTCGACA AATTCCATGC GCGCGACGCC GCATAGCTCG 
TCCTCGTTCA ATTTACGATC GCCGACGCGG GAACCGTCGG AATACCGTCA TCCACTTTCC 
TCTCTGGCGA CTCCAGCACC CTTTGCGGCC TCGCCTCCCA CATCCATTGC CAACGCCAAC 
AACACCAACA ACAACAACGC GCTAGGTGCC GCCGGCAGTC TGAGCAGTCA ACCACCACCA 
CCACCACCCA CGGGTCCGCG CTCAATTCTT AATCCTCCAA CACCAAGCCA ACAACATCAA 
CAACAACACC ACCATCCCAA TCCCTTTGTC GCAGCATCCG CACCCTCGCT GCCGCCGCCG 
CCGTCCTCGT TGCAAGCTCC ACCTGCCATT ACACCCATCG CCGGCCTATC AGCTCCGGCC 
CCGGCATCGG GTTCGCTGCC TCTGTCAGCA GGAGGCATCG GAAACTCCAT CACGGTCTCG 
TCCAGCTCCC AACCGCCAGC CCGAGCATCG CAACTGCACG CGCCGTCGGC CTACTATTCG 
CCGGCAGAAT CGTTCCGCGA TCGGGATTCG TCAGTACGCG AAAAATCATC GACAGGCGGC 
AGCTTTTACG ACCCAACCGC CGAGGCCAGC AACGGCATTA GTGGCTCGAG TCCACGAAAA 
GATCGAGATC GCGACCGTGA TCACCGGGGT ACAACAAGGG AATCACAGCG CCGCAGTGTT 
TCGGGCCATT CCGACACGGG CTCCTCCTGG CGAAACGCGA CCCAGACTTC CGCCAGCAAC 
AAGACCCGCG ATCCGTACAA CTATTCCCCG TCCTCGGCCG ATTATTATAA CACCAGGAAA 
AAGGAAAACT ACCCGGTCGA TAATACGACG TCTTCATCGA TTGCAGCTCC GTCCAACTTT 
ACTGTTGCGA CGCGTAGTCC TGTAGCCGCG CTTTCCCATC CGGCGTCCAT AGCTGCACCA 
GCTAGTGTCG GCAGTCTCAC CGGATCCATC AGCCCCAGGC TTTCACTCAG ACCACCAAGC 
ATGGCCTCTC CCACGATACG AAGCGCCGTC TTAGCTAATC CCACAAACGG CACAACTTCC 
ACCGCACTAC CGGCTCTAGG CAGGAACGAT TCGCCACCTT CCAAGATGTC TCCCGGTACA 
TCAACCGTAA GCTCTTTCGC TCTTCGTCCC CATGTACCAA TCGGCAATAA CCCTAGCCGT 
GCCGCTGGTG TCATGTCCTT TTCCAATATT CTCTCGAGCT CCGAGCCCGT TCCTAGACCC 
CGTGCGACTT CACCCAACAA CCCGGATGAT GACGACGACG TTCCCATGAA GGTCGAGCGC 
GCCGACTCAT CCGAGAAGGT CGTCAAGGAG AAGAAGGAGA GGAAACCGCG CCAGCCCAAG 
CAACCACGCA TCTCTGACAT CAGGCACTCC GAGTCCACCC CCAAAGGGCG ACGAGGGTCG 
ACCAAGCAAG AGAGTCCACT GCCCAACATT CGCATTCCGG CCAAGAGAAT GGCCAACGGT 
GCTCCCAAGC AACAAAAGAC ATTCTCGGCG GAGAATGAGG AGAAGATCAG GAAAGCGATG 
GATCGAATCG AAACACGAGA GCTTCCCCAC GAGGACGAAT TCGAAGAGGA GTTGCGCTTG 
TGGAGAGAGC GACGCGAGTA CAAGAGGCAA CAGATGAACC AACGCGACCT TCGCCAGCGT 
CGACAGAGGA GAGCCGACTA CACCGAAGTC GAAGCCCAGA AGCTCAAGCT TCATGCCGAT 
TTTGGCAAGC GTCGATATGA CGACCTTAAT TACGATGATG CGTTGCAGGA GGTTCGGGAG 
CGCGAGCTCT TTGCCGAGAA GGAGCGCAAG AAGGACATGC AGCGTAAGAG GCGGCGCGAG 
AAGTCCATGG CCACTACCAT GGAGGCAAAG GCAGCCGCGT TGGCCAGGGC CTCTGCAGCG 
CAGGATGAAG CCGAAAGACA GAAATACATG CGCGAAGCAG AACGGGCGAA CAAGAAGGTT 
CAACAGACCC GCCTCATTTT GCAGAAGGGC ATCAAGGGCC CGTCGCGGAA CACCGGTCCC 
ATTGAGCCGA ACCTGGAAGG CGGTACTATG GCTACGTTCC AGGCCGAGAA CATGGAGCCT 
GGTAAGACGA AGGGTAAGGG TCGTGCCGGC GCGAGACCCA AGAAATCCAA GGAGCAGAAG 
CAGGCCGAGA AGGATGCCGC CGAAGCTGCC CAAGCCGCTC TTGATGCCGG TCTCGAGCTG 
CCGCCCAAGG AAGAAACCAA CAAGATCCGC ATCAAGCTTA CCAAGACTAA GGCGCCCAAG 
GAGGCTGATG TGGACAAGGA TAAGGAGAAC AAGGAGCCTC AAGAGCCGAA GGAGCCTAAG 
GAGCCCAAGG AGAAGGTGAT AAAGGAAAAG GTTGTGGAGG AACCCAAGGA CCCGTTGGAA 
CTCAAGTTCC AGTCCAAGGG CTTCAACCAG ATCTATGACC AGATCTGGCG CGATCTTGCT 
AGGAAGGACG TCAACAAGGT GTTCAGATTG GCGATCGATT CGTATTCTAC CAAGTCCTCT 
AACCTCAAGA AGACTGCCAT CCTCGCGTCC AAAGAGGCCA AGAGATGGCA ACTCCGTACC 
AACAAGGGCA CCAAGGATTT GCAAGCTCGC GCCAAGCGTG TCATGCGTGA CATGATGGGC 
TTCTGGAAGC GCAACGAGCG TGAAGAGCGT GATCTCCGGA AGGCAGCTGA AAAGCAAGAG 
CTGGAGAACG CTCGCAAGGA GGAGGCAGAC CGCGAAGCCG CTCGCCAGAA GAGGAAGCTC 
AACTTCCTCA TCTCGCAGAC CGAACTGTAC TCGCATTTCA TTGGCAAGAA GATCAAGACC 
AACGAGGTGG AGCGCAGCAC GGATCACCCC GATGAGATCG CGGCTGAGAA GGACAAGATC 
CCGGAGAACG AGATGGACAT CGAGGTGCCT ACTGGCCCCA TTGGCGCCAA GGTCACCAAC 
TTTGAGAATC TGGACTTTGA CGCCGAAGAT GAATCGACGC TGCGGGCTGC TGCCATGGCG 
AATGCGCAGA ATGCCATTGC CGAGGCCCAG AAGAAGGCTA GGGAGTTCAA CAAGGAAGAG 
TCCAAGCTTG ATGAGGATGG CGAGATGAAC TTCCAGAACC CAACCATGAT GGGCGACGTC 
GAGATCGAGC AGCCCAAGCT TCTCAACTGC CAGCTCAAGG AGTACCAGCT CAAGGGTCTC 
AATTGGCTCG TCAACCTGTA CGAGCAAGGC ATAAACGGTA TCCTGGCCGA CGAAATGGGT 
CTAGGAAAGA CGGTGCAGTC CATTTCCGTC ATGGCCTACC TTGCCGAGAA GTACGATATC 
TGGGGTCCCT TCCTGGTTGT TGCGCCTGCA TCGACCCTAC ACAACTGGCA GCAGGAAATC 
ACAAAGTTCG TTCCCCAGTT CAAGGTGCTC CCTTACTGGG GTACTGCAGG CGACCGCAAG 
GTGCTGCGTA AGTTCTGGGA TCGCAAGCAC ACGACCTACA AGAAGGACGC CCCCTTTCAT 
GTCATGATTA CCTCGTACCA ACTGGTCGTC TCTGATGTTG CGTACTTCCA AAAGATGAAG 
TGGCAGTACA TGATCCTGGA TGAAGCCCAG GCCATCAAGA GTTCACAGAG TTCGCGTTGG 
AAGTGCCTGT TGGGCTTCCA CTGCCGTAAC CGTCTCCTGT TGACGGGTAC GCCTATCCAG 
AACAACATGC AGGAACTCTG GGCCCTGCTG CATTTCATCA TGCCGTCACT GTTCGATTCT 
CACGATGAGT TTAGTGAATG GTTCTCCAAG GATATCGAGT CTCATGCTCA GAGTAATACC 
AAGCTCAACG AGGACCAGCT GAAGCGTCTG CACATGATCC TCAAACCCTT TATGCTGCGT 
CGTGTCAAGA AGCATGTCCA AAAGGAACTC GGCGACAAGA TCGAGATGGA CGTTTTCTGC 
GATCTCACAT ACAGACAGCG TGCCATGTAC GCCAATTTGC GCAACCAGAT CAGCATCATG 
GACCTCATCG AGAAGGCGAC ACTGGGCGAC GACGATTCTG CTAGTCTCAT GAATCTCGTC 
ATGCAGTTCC GTAAGGTGTG CAACCACCCT GACCTCTTCG AGCGGGCGGA TACCGCTTCG 
CCGTACTCGT TCGGCCACTT TGCCGAGACA GCCTCGTTCA TCCGTGAGGG TAGCCAGGTT 
ACCGTCGGTT ACTCTACGCG CAGCTTGATC CAGTATGAGC TGCCTAGACT GCTCTGGCGC 
GACGGAGGAC GGCTGCACAA GGCTGGTGAA GACAACCAGG TCGCTGGATG GCGGAACCAG 
TGGCTCAATG AGAAGTTTAA CATCTGGACT CCGGAGCATA TCCGTGAGAG CCTAGCCGGG 
ACTGACAACT TCTCGTGGTT GCGCTTTGCC GACACGAGCT ACGAGGAGGC CTACCGGGCT 
AGCCATAAGG ATCTCTTTGC TCGGGCTGTT GAGATGTCCA CCAAGAAGAA CCGGCTCGCG 
GAGATCAAGA TCGCTTACGA TGAGCCGGAG GACTTGAACT TCACTCCGGC GCATGCCCTC 
TTCCACATCA GGGAACGTGA GGATCGGAGG CCGTTGGCTG AGATCACTGA GCAAGGCATT 
CTGGGCAGTT TGATGAATGT CTCCCGGAGT GCATTCTCCG AGACTGGCCT CGGTCGTCTC 
GAGCAGGCTG CGGCTCCCAA GGCCTCGGCT CCTCCTATCG AGGTGGTCTG CGACAGCCGT 
AGCGCAGTGG TTGAGCGGGA GAACATCATG TTCAACGCTC CCATGCGCAA GGTCCTCTTT 
GGACCTACCC TGGCCGAGGA GAAGGCGCTC GTCGTACAAA AGGTGCCGCC ATCACGCTAC 
CCACCGCCAG CGTTGCTTCC TGCACCAGAC AAGGAGAAGC AGAAGTTCAC CAACATCACC 
GTCCCGTCGA TGCGCCGCTT TGTCACCGAC TCGGGCAAGC TCGCCAAGCT CGACGAGCTC 
CTGCGCGAGC TCAAGGAGAA TGGCCACCGC GTCCTCCTCT ACTTCCAAAT GACGCGCATG 
ATCGATCTCA TGGAAGAGTA TCTTACTTAC CGCAACTACA AGTACTGCCG ACTCGACGGT 
TCAACCAAGC TCGAAGACCG TCGCGACACC GTTGCCGACT TCCAGACCCG CCCCGAGATC 
TTCATCTTCC TTTTGTCTAC TCGTGCCGGT GGTCTGGGCA TCAACCTGAC CTCTGCCGAC 
ACCGTCATCT TCTACGACTC GGACTGGAAC CCGACCATCG ATTCGCAAGC CATGGATCGT 
GCCCATCGTC TGGGTCAGAC CAAACAGGTT ACTGTCTACC GTCTTATCAC CAGGGGCACG 
ATCGAGGAAC GCATCCGCAA GCGTGCCATG CAAAAGGAAG AAGTCCAGCG CGTGGTTATC 
ACGGGTGGCT CATCGGCTGC TGGTGGCGGT GTGGACTTCT CTGGTCGTCG TGCGCCTGAG 
AACAGGAACC GTGACATTGC CATGTGGCTG GCGGATGACG AGCAGGCGGA GATGATTGAG 
AAGAGGGAGC GCGAGCTGTT GGAGAGTGGA GAGTTGGATA AGATGCAGAA GAAGAGTAGG 
GGAGGTAACA AGAGGAAGAG AGGAGGTGCT GGGGGAGAAG GGAAGGAGGT TAGTTTGGAT 
GAGATGTATC ATGAGGGTGA GGGCAACTTT GATGATGGAG GAAATAATAT CAAGGGTAGC 
GGAACGGCTA CGCCTAATGG TGCTGCCGGT GGTGAGGGAG GTGATGGGAA GGGAGCAGTG 
GGAGGAGCGG CGAAGAAGAG GAAGACGGGT GGAAGCAAGA AGGCCAAGAC GACCAAGCAG 
AGATTGGCTA TTGCTGACGG GGAGATTGAT ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011394746.1
CDS	987..7022
Translation	MDHFSTVLQRPPHFDEDGTEGRGDRGNGAGPGPAPPPPPPRGGLRDILNPVSSNSAVQSQAAAAAPPPPPAVASSSLHGIAASVPPPSSTNSMRATPHSSSSFNLRSPTREPSEYRHPLSSLATPAPFAASPPTSIANANNTNNNNALGAAGSLSSQPPPPPPTGPRSILNPPTPSQQHQQQHHHPNPFVAASAPSLPPPPSSLQAPPAITPIAGLSAPAPASGSLPLSAGGIGNSITVSSSSQPPARASQLHAPSAYYSPAESFRDRDSSVREKSSTGGSFYDPTAEASNGISGSSPRKDRDRDRDHRGTTRESQRRSVSGHSDTGSSWRNATQTSASNKTRDPYNYSPSSADYYNTRKKENYPVDNTTSSSIAAPSNFTVATRSPVAALSHPASIAAPASVGSLTGSISPRLSLRPPSMASPTIRSAVLANPTNGTTSTALPALGRNDSPPSKMSPGTSTVSSFALRPHVPIGNNPSRAAGVMSFSNILSSSEPVPRPRATSPNNPDDDDDVPMKVERADSSEKVVKEKKERKPRQPKQPRISDIRHSESTPKGRRGSTKQESPLPNIRIPAKRMANGAPKQQKTFSAENEEKIRKAMDRIETRELPHEDEFEEELRLWRERREYKRQQMNQRDLRQRRQRRADYTEVEAQKLKLHADFGKRRYDDLNYDDALQEVRERELFAEKERKKDMQRKRRREKSMATTMEAKAAALARASAAQDEAERQKYMREAERANKKVQQTRLILQKGIKGPSRNTGPIEPNLEGGTMATFQAENMEPGKTKGKGRAGARPKKSKEQKQAEKDAAEAAQAALDAGLELPPKEETNKIRIKLTKTKAPKEADVDKDKENKEPQEPKEPKEPKEKVIKEKVVEEPKDPLELKFQSKGFNQIYDQIWRDLARKDVNKVFRLAIDSYSTKSSNLKKTAILASKEAKRWQLRTNKGTKDLQARAKRVMRDMMGFWKRNEREERDLRKAAEKQELENARKEEADREAARQKRKLNFLISQTELYSHFIGKKIKTNEVERSTDHPDEIAAEKDKIPENEMDIEVPTGPIGAKVTNFENLDFDAEDESTLRAAAMANAQNAIAEAQKKAREFNKEESKLDEDGEMNFQNPTMMGDVEIEQPKLLNCQLKEYQLKGLNWLVNLYEQGINGILADEMGLGKTVQSISVMAYLAEKYDIWGPFLVVAPASTLHNWQQEITKFVPQFKVLPYWGTAGDRKVLRKFWDRKHTTYKKDAPFHVMITSYQLVVSDVAYFQKMKWQYMILDEAQAIKSSQSSRWKCLLGFHCRNRLLLTGTPIQNNMQELWALLHFIMPSLFDSHDEFSEWFSKDIESHAQSNTKLNEDQLKRLHMILKPFMLRRVKKHVQKELGDKIEMDVFCDLTYRQRAMYANLRNQISIMDLIEKATLGDDDSASLMNLVMQFRKVCNHPDLFERADTASPYSFGHFAETASFIREGSQVTVGYSTRSLIQYELPRLLWRDGGRLHKAGEDNQVAGWRNQWLNEKFNIWTPEHIRESLAGTDNFSWLRFADTSYEEAYRASHKDLFARAVEMSTKKNRLAEIKIAYDEPEDLNFTPAHALFHIREREDRRPLAEITEQGILGSLMNVSRSAFSETGLGRLEQAAAPKASAPPIEVVCDSRSAVVERENIMFNAPMRKVLFGPTLAEEKALVVQKVPPSRYPPPALLPAPDKEKQKFTNITVPSMRRFVTDSGKLAKLDELLRELKENGHRVLLYFQMTRMIDLMEEYLTYRNYKYCRLDGSTKLEDRRDTVADFQTRPEIFIFLLSTRAGGLGINLTSADTVIFYDSDWNPTIDSQAMDRAHRLGQTKQVTVYRLITRGTIEERIRKRAMQKEEVQRVVITGGSSAAGGGVDFSGRRAPENRNRDIAMWLADDEQAEMIEKRERELLESGELDKMQKKSRGGNKRKRGGAGGEGKEVSLDEMYHEGEGNFDDGGNNIKGSGTATPNGAAGGEGGDGKGAVGGAAKKRKTGGSKKAKTTKQRLAIADGEIDI

Target ORF information:

RefSeq Version	XM_011396444.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A SNF2 family helicase/ATPase (crf2-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011396444.1

ORF Insert Sequence:

ATGGACCACT TCTCGACAGT GCTGCAGCGA CCTCCTCATT TTGACGAGGA TGGAACAGAA 
GGGAGAGGAG ATCGAGGAAA CGGCGCCGGT CCAGGTCCAG CACCACCACC ACCACCACCA 
CGCGGAGGTC TGCGCGACAT TCTCAACCCA GTGAGCAGCA ACAGCGCCGT ACAATCCCAA 
GCAGCAGCAG CAGCACCACC ACCACCACCA GCAGTAGCGT CATCATCGCT CCATGGAATT 
GCAGCATCAG TGCCGCCGCC CTCGTCGACA AATTCCATGC GCGCGACGCC GCATAGCTCG 
TCCTCGTTCA ATTTACGATC GCCGACGCGG GAACCGTCGG AATACCGTCA TCCACTTTCC 
TCTCTGGCGA CTCCAGCACC CTTTGCGGCC TCGCCTCCCA CATCCATTGC CAACGCCAAC 
AACACCAACA ACAACAACGC GCTAGGTGCC GCCGGCAGTC TGAGCAGTCA ACCACCACCA 
CCACCACCCA CGGGTCCGCG CTCAATTCTT AATCCTCCAA CACCAAGCCA ACAACATCAA 
CAACAACACC ACCATCCCAA TCCCTTTGTC GCAGCATCCG CACCCTCGCT GCCGCCGCCG 
CCGTCCTCGT TGCAAGCTCC ACCTGCCATT ACACCCATCG CCGGCCTATC AGCTCCGGCC 
CCGGCATCGG GTTCGCTGCC TCTGTCAGCA GGAGGCATCG GAAACTCCAT CACGGTCTCG 
TCCAGCTCCC AACCGCCAGC CCGAGCATCG CAACTGCACG CGCCGTCGGC CTACTATTCG 
CCGGCAGAAT CGTTCCGCGA TCGGGATTCG TCAGTACGCG AAAAATCATC GACAGGCGGC 
AGCTTTTACG ACCCAACCGC CGAGGCCAGC AACGGCATTA GTGGCTCGAG TCCACGAAAA 
GATCGAGATC GCGACCGTGA TCACCGGGGT ACAACAAGGG AATCACAGCG CCGCAGTGTT 
TCGGGCCATT CCGACACGGG CTCCTCCTGG CGAAACGCGA CCCAGACTTC CGCCAGCAAC 
AAGACCCGCG ATCCGTACAA CTATTCCCCG TCCTCGGCCG ATTATTATAA CACCAGGAAA 
AAGGAAAACT ACCCGGTCGA TAATACGACG TCTTCATCGA TTGCAGCTCC GTCCAACTTT 
ACTGTTGCGA CGCGTAGTCC TGTAGCCGCG CTTTCCCATC CGGCGTCCAT AGCTGCACCA 
GCTAGTGTCG GCAGTCTCAC CGGATCCATC AGCCCCAGGC TTTCACTCAG ACCACCAAGC 
ATGGCCTCTC CCACGATACG AAGCGCCGTC TTAGCTAATC CCACAAACGG CACAACTTCC 
ACCGCACTAC CGGCTCTAGG CAGGAACGAT TCGCCACCTT CCAAGATGTC TCCCGGTACA 
TCAACCGTAA GCTCTTTCGC TCTTCGTCCC CATGTACCAA TCGGCAATAA CCCTAGCCGT 
GCCGCTGGTG TCATGTCCTT TTCCAATATT CTCTCGAGCT CCGAGCCCGT TCCTAGACCC 
CGTGCGACTT CACCCAACAA CCCGGATGAT GACGACGACG TTCCCATGAA GGTCGAGCGC 
GCCGACTCAT CCGAGAAGGT CGTCAAGGAG AAGAAGGAGA GGAAACCGCG CCAGCCCAAG 
CAACCACGCA TCTCTGACAT CAGGCACTCC GAGTCCACCC CCAAAGGGCG ACGAGGGTCG 
ACCAAGCAAG AGAGTCCACT GCCCAACATT CGCATTCCGG CCAAGAGAAT GGCCAACGGT 
GCTCCCAAGC AACAAAAGAC ATTCTCGGCG GAGAATGAGG AGAAGATCAG GAAAGCGATG 
GATCGAATCG AAACACGAGA GCTTCCCCAC GAGGACGAAT TCGAAGAGGA GTTGCGCTTG 
TGGAGAGAGC GACGCGAGTA CAAGAGGCAA CAGATGAACC AACGCGACCT TCGCCAGCGT 
CGACAGAGGA GAGCCGACTA CACCGAAGTC GAAGCCCAGA AGCTCAAGCT TCATGCCGAT 
TTTGGCAAGC GTCGATATGA CGACCTTAAT TACGATGATG CGTTGCAGGA GGTTCGGGAG 
CGCGAGCTCT TTGCCGAGAA GGAGCGCAAG AAGGACATGC AGCGTAAGAG GCGGCGCGAG 
AAGTCCATGG CCACTACCAT GGAGGCAAAG GCAGCCGCGT TGGCCAGGGC CTCTGCAGCG 
CAGGATGAAG CCGAAAGACA GAAATACATG CGCGAAGCAG AACGGGCGAA CAAGAAGGTT 
CAACAGACCC GCCTCATTTT GCAGAAGGGC ATCAAGGGCC CGTCGCGGAA CACCGGTCCC 
ATTGAGCCGA ACCTGGAAGG CGGTACTATG GCTACGTTCC AGGCCGAGAA CATGGAGCCT 
GGTAAGACGA AGGGTAAGGG TCGTGCCGGC GCGAGACCCA AGAAATCCAA GGAGCAGAAG 
CAGGCCGAGA AGGATGCCGC CGAAGCTGCC CAAGCCGCTC TTGATGCCGG TCTCGAGCTG 
CCGCCCAAGG AAGAAACCAA CAAGATCCGC ATCAAGCTTA CCAAGACTAA GGCGCCCAAG 
GAGGCTGATG TGGACAAGGA TAAGGAGAAC AAGGAGCCTC AAGAGCCGAA GGAGCCTAAG 
GAGCCCAAGG AGAAGGTGAT AAAGGAAAAG GTTGTGGAGG AACCCAAGGA CCCGTTGGAA 
CTCAAGTTCC AGTCCAAGGG CTTCAACCAG ATCTATGACC AGATCTGGCG CGATCTTGCT 
AGGAAGGACG TCAACAAGGT GTTCAGATTG GCGATCGATT CGTATTCTAC CAAGTCCTCT 
AACCTCAAGA AGACTGCCAT CCTCGCGTCC AAAGAGGCCA AGAGATGGCA ACTCCGTACC 
AACAAGGGCA CCAAGGATTT GCAAGCTCGC GCCAAGCGTG TCATGCGTGA CATGATGGGC 
TTCTGGAAGC GCAACGAGCG TGAAGAGCGT GATCTCCGGA AGGCAGCTGA AAAGCAAGAG 
CTGGAGAACG CTCGCAAGGA GGAGGCAGAC CGCGAAGCCG CTCGCCAGAA GAGGAAGCTC 
AACTTCCTCA TCTCGCAGAC CGAACTGTAC TCGCATTTCA TTGGCAAGAA GATCAAGACC 
AACGAGGTGG AGCGCAGCAC GGATCACCCC GATGAGATCG CGGCTGAGAA GGACAAGATC 
CCGGAGAACG AGATGGACAT CGAGGTGCCT ACTGGCCCCA TTGGCGCCAA GGTCACCAAC 
TTTGAGAATC TGGACTTTGA CGCCGAAGAT GAATCGACGC TGCGGGCTGC TGCCATGGCG 
AATGCGCAGA ATGCCATTGC CGAGGCCCAG AAGAAGGCTA GGGAGTTCAA CAAGGAAGAG 
TCCAAGCTTG ATGAGGATGG CGAGATGAAC TTCCAGAACC CAACCATGAT GGGCGACGTC 
GAGATCGAGC AGCCCAAGCT TCTCAACTGC CAGCTCAAGG AGTACCAGCT CAAGGGTCTC 
AATTGGCTCG TCAACCTGTA CGAGCAAGGC ATAAACGGTA TCCTGGCCGA CGAAATGGGT 
CTAGGAAAGA CGGTGCAGTC CATTTCCGTC ATGGCCTACC TTGCCGAGAA GTACGATATC 
TGGGGTCCCT TCCTGGTTGT TGCGCCTGCA TCGACCCTAC ACAACTGGCA GCAGGAAATC 
ACAAAGTTCG TTCCCCAGTT CAAGGTGCTC CCTTACTGGG GTACTGCAGG CGACCGCAAG 
GTGCTGCGTA AGTTCTGGGA TCGCAAGCAC ACGACCTACA AGAAGGACGC CCCCTTTCAT 
GTCATGATTA CCTCGTACCA ACTGGTCGTC TCTGATGTTG CGTACTTCCA AAAGATGAAG 
TGGCAGTACA TGATCCTGGA TGAAGCCCAG GCCATCAAGA GTTCACAGAG TTCGCGTTGG 
AAGTGCCTGT TGGGCTTCCA CTGCCGTAAC CGTCTCCTGT TGACGGGTAC GCCTATCCAG 
AACAACATGC AGGAACTCTG GGCCCTGCTG CATTTCATCA TGCCGTCACT GTTCGATTCT 
CACGATGAGT TTAGTGAATG GTTCTCCAAG GATATCGAGT CTCATGCTCA GAGTAATACC 
AAGCTCAACG AGGACCAGCT GAAGCGTCTG CACATGATCC TCAAACCCTT TATGCTGCGT 
CGTGTCAAGA AGCATGTCCA AAAGGAACTC GGCGACAAGA TCGAGATGGA CGTTTTCTGC 
GATCTCACAT ACAGACAGCG TGCCATGTAC GCCAATTTGC GCAACCAGAT CAGCATCATG 
GACCTCATCG AGAAGGCGAC ACTGGGCGAC GACGATTCTG CTAGTCTCAT GAATCTCGTC 
ATGCAGTTCC GTAAGGTGTG CAACCACCCT GACCTCTTCG AGCGGGCGGA TACCGCTTCG 
CCGTACTCGT TCGGCCACTT TGCCGAGACA GCCTCGTTCA TCCGTGAGGG TAGCCAGGTT 
ACCGTCGGTT ACTCTACGCG CAGCTTGATC CAGTATGAGC TGCCTAGACT GCTCTGGCGC 
GACGGAGGAC GGCTGCACAA GGCTGGTGAA GACAACCAGG TCGCTGGATG GCGGAACCAG 
TGGCTCAATG AGAAGTTTAA CATCTGGACT CCGGAGCATA TCCGTGAGAG CCTAGCCGGG 
ACTGACAACT TCTCGTGGTT GCGCTTTGCC GACACGAGCT ACGAGGAGGC CTACCGGGCT 
AGCCATAAGG ATCTCTTTGC TCGGGCTGTT GAGATGTCCA CCAAGAAGAA CCGGCTCGCG 
GAGATCAAGA TCGCTTACGA TGAGCCGGAG GACTTGAACT TCACTCCGGC GCATGCCCTC 
TTCCACATCA GGGAACGTGA GGATCGGAGG CCGTTGGCTG AGATCACTGA GCAAGGCATT 
CTGGGCAGTT TGATGAATGT CTCCCGGAGT GCATTCTCCG AGACTGGCCT CGGTCGTCTC 
GAGCAGGCTG CGGCTCCCAA GGCCTCGGCT CCTCCTATCG AGGTGGTCTG CGACAGCCGT 
AGCGCAGTGG TTGAGCGGGA GAACATCATG TTCAACGCTC CCATGCGCAA GGTCCTCTTT 
GGACCTACCC TGGCCGAGGA GAAGGCGCTC GTCGTACAAA AGGTGCCGCC ATCACGCTAC 
CCACCGCCAG CGTTGCTTCC TGCACCAGAC AAGGAGAAGC AGAAGTTCAC CAACATCACC 
GTCCCGTCGA TGCGCCGCTT TGTCACCGAC TCGGGCAAGC TCGCCAAGCT CGACGAGCTC 
CTGCGCGAGC TCAAGGAGAA TGGCCACCGC GTCCTCCTCT ACTTCCAAAT GACGCGCATG 
ATCGATCTCA TGGAAGAGTA TCTTACTTAC CGCAACTACA AGTACTGCCG ACTCGACGGT 
TCAACCAAGC TCGAAGACCG TCGCGACACC GTTGCCGACT TCCAGACCCG CCCCGAGATC 
TTCATCTTCC TTTTGTCTAC TCGTGCCGGT GGTCTGGGCA TCAACCTGAC CTCTGCCGAC 
ACCGTCATCT TCTACGACTC GGACTGGAAC CCGACCATCG ATTCGCAAGC CATGGATCGT 
GCCCATCGTC TGGGTCAGAC CAAACAGGTT ACTGTCTACC GTCTTATCAC CAGGGGCACG 
ATCGAGGAAC GCATCCGCAA GCGTGCCATG CAAAAGGAAG AAGTCCAGCG CGTGGTTATC 
ACGGGTGGCT CATCGGCTGC TGGTGGCGGT GTGGACTTCT CTGGTCGTCG TGCGCCTGAG 
AACAGGAACC GTGACATTGC CATGTGGCTG GCGGATGACG AGCAGGCGGA GATGATTGAG 
AAGAGGGAGC GCGAGCTGTT GGAGAGTGGA GAGTTGGATA AGATGCAGAA GAAGAGTAGG 
GGAGGTAACA AGAGGAAGAG AGGAGGTGCT GGGGGAGAAG GGAAGGAGGT TAGTTTGGAT 
GAGATGTATC ATGAGGGTGA GGGCAACTTT GATGATGGAG GAAATAATAT CAAGGGTAGC 
GGAACGGCTA CGCCTAATGG TGCTGCCGGT GGTGAGGGAG GTGATGGGAA GGGAGCAGTG 
GGAGGAGCGG CGAAGAAGAG GAAGACGGGT GGAAGCAAGA AGGCCAAGAC GACCAAGCAG 
AGATTGGCTA TTGCTGACGG GGAGATTGAT ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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crf2-1 cDNA ORF clone, Neurospora crassa OR74A

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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