en(am)-2 cDNA ORF clone, Neurospora crassa OR74A

The following en(am)-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the en(am)-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ONf04205 XM_011395198.1
Latest version!
Neurospora crassa OR74A glutamate synthase, variant (en(am)-2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
ONf04205 XM_011395197.1
Latest version!
Neurospora crassa OR74A glutamate synthase (en(am)-2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ONf04205
    Clone ID Related Accession (Same CDS sequence) XM_011395197.1 , XM_011395198.1
    Accession Version XM_011395197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product glutamate synthase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
    TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG
    AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC
    CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC
    ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG
    ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT
    CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG
    CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA
    TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT
    TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA
    ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG
    AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC
    AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT
    TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC
    ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA
    AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT
    GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT
    GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG
    GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG
    GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT
    ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC
    CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC
    GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT
    GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG
    GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG
    CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG
    GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC
    ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT
    CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC
    AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT
    CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC
    CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG
    TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT
    AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC
    ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT
    GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC
    GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT
    GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT
    ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT
    GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC
    CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT
    GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC
    GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG
    TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC
    CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC
    GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT
    CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT
    GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC
    AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA
    CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT
    GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC
    AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT
    CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG
    ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT
    TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT
    AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT
    GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG
    GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC
    CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT
    CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT
    ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC
    AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC
    ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC
    AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC
    GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC
    AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT
    GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA
    TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC
    CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC
    GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT
    GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC
    GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC
    ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC
    GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC
    ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC
    ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT
    CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC
    AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC
    AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG
    AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT
    AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT
    CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG
    TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT
    GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT
    GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC
    TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC
    ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC
    GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC
    ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC
    GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC
    ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC
    CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA
    AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC
    CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT
    CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT
    ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG
    TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG
    TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC
    ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC
    GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT
    TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG
    TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT
    CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT
    GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG
    GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT
    ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393499.1
    CDS487..6837
    Translation

    Target ORF information:

    RefSeq Version XM_011395197.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A glutamate synthase (en(am)-2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011395197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
    TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG
    AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC
    CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC
    ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG
    ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT
    CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG
    CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA
    TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT
    TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA
    ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG
    AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC
    AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT
    TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC
    ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA
    AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT
    GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT
    GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG
    GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG
    GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT
    ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC
    CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC
    GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT
    GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG
    GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG
    CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG
    GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC
    ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT
    CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC
    AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT
    CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC
    CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG
    TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT
    AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC
    ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT
    GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC
    GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT
    GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT
    ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT
    GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC
    CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT
    GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC
    GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG
    TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC
    CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC
    GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT
    CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT
    GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC
    AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA
    CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT
    GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC
    AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT
    CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG
    ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT
    TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT
    AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT
    GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG
    GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC
    CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT
    CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT
    ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC
    AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC
    ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC
    AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC
    GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC
    AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT
    GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA
    TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC
    CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC
    GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT
    GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC
    GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC
    ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC
    GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC
    ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC
    ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT
    CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC
    AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC
    AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG
    AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT
    AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT
    CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG
    TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT
    GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT
    GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC
    TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC
    ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC
    GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC
    ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC
    GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC
    ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC
    CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA
    AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC
    CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT
    CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT
    ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG
    TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG
    TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC
    ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC
    GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT
    TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG
    TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT
    CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT
    GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG
    GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT
    ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ONf04205
    Clone ID Related Accession (Same CDS sequence) XM_011395197.1 , XM_011395198.1
    Accession Version XM_011395198.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-10
    Organism Neurospora crassa OR74A
    Product glutamate synthase, variant
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
    TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG
    AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC
    CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC
    ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG
    ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT
    CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG
    CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA
    TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT
    TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA
    ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG
    AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC
    AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT
    TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC
    ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA
    AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT
    GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT
    GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG
    GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG
    GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT
    ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC
    CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC
    GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT
    GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG
    GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG
    CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG
    GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC
    ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT
    CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC
    AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT
    CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC
    CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG
    TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT
    AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC
    ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT
    GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC
    GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT
    GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT
    ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT
    GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC
    CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT
    GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC
    GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG
    TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC
    CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC
    GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT
    CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT
    GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC
    AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA
    CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT
    GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC
    AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT
    CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG
    ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT
    TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT
    AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT
    GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG
    GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC
    CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT
    CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT
    ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC
    AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC
    ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC
    AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC
    GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC
    AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT
    GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA
    TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC
    CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC
    GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT
    GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC
    GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC
    ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC
    GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC
    ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC
    ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT
    CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC
    AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC
    AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG
    AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT
    AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT
    CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG
    TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT
    GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT
    GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC
    TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC
    ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC
    GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC
    ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC
    GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC
    ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC
    CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA
    AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC
    CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT
    CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT
    ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG
    TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG
    TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC
    ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC
    GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT
    TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG
    TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT
    CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT
    GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG
    GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT
    ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011393500.1
    CDS483..6833
    Translation

    Target ORF information:

    RefSeq Version XM_011395198.1
    Organism Neurospora crassa OR74A
    Definition Neurospora crassa OR74A glutamate synthase, variant (en(am)-2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011395198.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
    TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG
    AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC
    CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC
    ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG
    ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT
    CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG
    CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA
    TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT
    TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA
    ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG
    AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC
    AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT
    TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC
    ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA
    AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT
    GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT
    GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG
    GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG
    GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT
    ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC
    CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC
    GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT
    GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG
    GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG
    CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG
    GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC
    ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT
    CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC
    AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT
    CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC
    CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG
    TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT
    AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC
    ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT
    GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC
    GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT
    GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT
    ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT
    GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC
    CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT
    GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC
    GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG
    TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC
    CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC
    GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT
    CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT
    GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC
    AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA
    CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT
    GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC
    AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT
    CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG
    ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT
    TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT
    AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT
    GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG
    GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC
    CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT
    CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT
    ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC
    AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC
    ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC
    AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC
    GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC
    AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT
    GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA
    TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC
    CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC
    GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT
    GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC
    GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC
    ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC
    GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC
    ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC
    ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT
    CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC
    AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC
    AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG
    AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT
    AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT
    CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG
    TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT
    GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT
    GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC
    TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC
    ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC
    GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC
    ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC
    GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC
    ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC
    CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA
    AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC
    CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT
    CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT
    ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG
    TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG
    TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC
    ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC
    GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT
    TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG
    TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT
    CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT
    GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG
    GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT
    ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the filamentous fungus Neurospora crassa.
    Nature422(6934)859-68(2003 Apr)
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