en(am)-2 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	en(am)-2
Entrez Gene ID	3873319
Full Name	enhancer-2 of am
Synonyms	en-am
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_011395197.1	XP_011393499.1	glutamate synthase
XM_011395198.1	XP_011393500.1	glutamate synthase, variant

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG9674	NP_001246804.1
Schizosaccharomyces pombe (fission yeast)	glt1	NP_594133.1
Magnaporthe oryzae (rice blast fungus)	MGG_07187	XP_003715392.1
Neurospora crassa	NCU01744	XP_957167.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006360	XP_316385.4
Caenorhabditis elegans (roundworm)	W07E11.1	NP_509693.2
Kluyveromyces lactis	KLLA0E19625g	XP_454839.1
Eremothecium gossypii	AGOS_ADR290W	NP_984386.2
Arabidopsis thaliana (thale cress)	GLT1	NP_200158.2
Oryza sativa (rice)	Os01g0681900	NP_001043884.1
Saccharomyces cerevisiae (baker's yeast)	GLT1	NP_010110.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following en(am)-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the en(am)-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf04205	XM_011395198.1 Latest version!	Neurospora crassa OR74A glutamate synthase, variant (en(am)-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ONf04205	XM_011395197.1 Latest version!	Neurospora crassa OR74A glutamate synthase (en(am)-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

en(am)-2 ( XM_011395197.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393499.1 Neurospora crassa OR74A glutamate synthase (en(am)-2), mRNA.

Price & Availability↑

CloneID	ONf04205
Clone ID Related Accession (Same CDS sequence)	XM_011395197.1 , XM_011395198.1
Accession Version	XM_011395197.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	glutamate synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG 
AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC 
CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC 
ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG 
ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT 
CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG 
CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA 
TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT 
TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA 
ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG 
AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC 
AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT 
TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC 
ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA 
AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT 
GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT 
GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG 
GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG 
GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT 
ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC 
CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC 
GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT 
GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG 
GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG 
CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG 
GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC 
ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT 
CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC 
AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT 
CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC 
CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG 
TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT 
AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC 
ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT 
GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC 
GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT 
GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT 
ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT 
GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC 
CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT 
GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC 
GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG 
TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC 
CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC 
GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT 
CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT 
GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC 
AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA 
CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT 
GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC 
AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT 
CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG 
ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT 
TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT 
AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT 
GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG 
GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC 
CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT 
CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT 
ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC 
AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC 
ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC 
AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC 
GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC 
AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT 
GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA 
TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC 
CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC 
GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT 
GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC 
GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC 
ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC 
GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC 
ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC 
ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT 
CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC 
AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC 
AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG 
AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT 
AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT 
CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG 
TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT 
GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT 
GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC 
TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC 
ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC 
GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC 
ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC 
GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC 
ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC 
CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA 
AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC 
CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT 
CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT 
ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG 
TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG 
TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC 
ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC 
GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT 
TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG 
TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT 
CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT 
GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG 
GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT 
ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393499.1
CDS	487..6837
Translation	MGAIEDFDDRQVHLETEKEQYHPYEYQTENNSSWAGALPVKQGLYDPELEKDACGVGFACHIKGKPSHKIVSDARNLLCNMTHRGAVGSDARDGDGAGVMTSIPHKFFVKNFARDVGVTLPPLGQYAVGNLFFKPDPETLQESKRQLEEIAESLGLRVLGWREPPVDSTLLGPAALSREPSILQPFVVLASAYGPGNEPETTDPEKFDERLFERQLYVLRKRATHTIGLQNWFYICSLSNKNIVYKGQLAPIQVYQYYNDLVNYDYEAHFALVHSRFSTNTFPSWDRAQPLRWAAHNGEINTLRGNKNWMRAREGVMQSDVFGDELELLYPIVEAGGSDSAAFDNVLELLTINGVLSLPEAVMLMVPEAWQGNQSMDPKKAAFYEWAACQMEPWDGPALFTFADGRFCGANLDRNGLRPCRYYVMDDDRIICASEVGTIPVEPEKIIQKGRLQPGRMLLVDTRAGRIIDDSELKAAVSSREDFRSWLDNELITMPQVLETLSGSQSIELAAKPDNTKIQEDPLLHAFGYTFEQVSLLLGPMASDEKEALGSMGNDAPLACLSDAPKLLYEYFRQLFAQVTNPPIDPIRESIVMSLECYVGPQGNLLEMDSSQCGRLLLPSPILSIPEFNALKNMSTIHPEWTVKTIDLTFPKKEGIEGYIRHLDYICMEATAAIEASDRIIVLSDRNTSKDRVAVSALLAGGMVHHHLVSNKWRAMAAIVAETAEAREVHHMCVLLGYGVDAINPYLAMECIIKLNKEKLIKKKLTDDELIYNYKHSCDGGILKVMSKMGISTLASYKGAQIFEALGVDDSVVERCFKGTASRIKGITFELIAEDAFRLHERGFPSRYTVAVPGLPESGEYHWRDNGEAHVNDPTSIANIQDAVRTKNDKSYEAYSLSEYEQIKSCTLRGMLDFNFEDCSPIPIDQVEPWTEIVRRFCTGAMSYGSISLESHSTLAVAMNRLGGKSNTGEGGEDPERSQRLPNGDTMRSAIKQVASGRFGVTSAYLADSDELQIKMAQGAKPGEGGELPGHKVSKSIARTRHSTPGVGLISPPPHHDIYSIEDLKQLIYDLKCSSPRSRVSVKLVSETGVGIVASGVAKAKADHILISGHDGGTGASRWTGIKYAGLPWELGLAETHQTLVLNDLRGRVVVQTDGQLRTGRDVAIACLLGAEEWGFATTPLIAMGCIMMRKCHLNTCPVGIATQDPELRKKFTGTPEHVINFFYYVANELRAIMARLGFRTINEMVGHVEVLKVREDLRTKKTSNIDLSLILTPAHKLRPGVATFNVRKQDHKLYVRLDNKLITEAELTLDKGLPSRIECDIVNTDRAMGTSLSYQISKRYGEKGLPDDTVHVNIKGSAGQSFGAFLAPGVTLELEGDANDYVGKGLSGGRLIVYPPRSAVFKAEENIIVGNVCLYGATSGTCFFRGVAAERFAVRNSGATAVVEGVGDHGCEYMTGGRVVILGSTGRNFAAGMSGGIAYILDIQGDFLTKLNTEMVEASTLEEAEPEEIAFIRSLVEDHHHYTGSELAARILVDFSRALKRFIKVLPVDYKRVLEEEKAKAVKQAAETKRAEYSLPAAARKEKDPHAPKLQDIEESITDNAISKKKALVLDKTRGFMKYQRRAEKYRKPATRVKDWAELSQRLDEDELKYQSARCMDCGVPFCQSDSGCPISNIIPKWNELVFQGQWRDALNRLLMTNNFPEFTGRVCPAPCEGACVLGINEQPVGIKSIECAIIDRGFEMGWMVPEPPKIRTGKNVAIIGSGPAGLAAADQLNKAGHNVTVYERADRLGGLLMYGIPNMKLDKRIVKRRTDFMAAEGVQFKTGVAIGEDITLVDLKKQNDAVIIATGATVARDLPIKNRNLEGIHFAMEFLHKNTKSLLDSELADGSYISAKDKHVIVIGGGDTGNDCIGTSVRHGAKSVINFELLPKPPPARGRDNPWPQWPRVYRVDYGHDEVKQHTGKDPREYCIMSEEFVDDGNGKVRGINTIRVEWTKSASGGWDMKKIEGSQQFFPADLVLLSMGFLGPEARVLGDEIEKDPRKNVKTPPGKYSTNVEGVFAAGDCRRGQSLIVWGINEGRQAAREVDLYLEKSTCLPVTGGIVKRTAEEVFAAAAAA

Target ORF information:

RefSeq Version	XM_011395197.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A glutamate synthase (en(am)-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395197.1

ORF Insert Sequence:

ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG 
AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC 
CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC 
ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG 
ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT 
CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG 
CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA 
TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT 
TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA 
ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG 
AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC 
AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT 
TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC 
ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA 
AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT 
GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT 
GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG 
GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG 
GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT 
ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC 
CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC 
GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT 
GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG 
GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG 
CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG 
GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC 
ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT 
CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC 
AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT 
CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC 
CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG 
TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT 
AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC 
ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT 
GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC 
GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT 
GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT 
ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT 
GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC 
CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT 
GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC 
GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG 
TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC 
CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC 
GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT 
CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT 
GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC 
AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA 
CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT 
GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC 
AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT 
CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG 
ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT 
TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT 
AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT 
GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG 
GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC 
CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT 
CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT 
ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC 
AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC 
ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC 
AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC 
GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC 
AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT 
GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA 
TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC 
CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC 
GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT 
GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC 
GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC 
ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC 
GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC 
ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC 
ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT 
CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC 
AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC 
AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG 
AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT 
AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT 
CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG 
TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT 
GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT 
GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC 
TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC 
ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC 
GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC 
ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC 
GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC 
ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC 
CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA 
AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC 
CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT 
CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT 
ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG 
TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG 
TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC 
ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC 
GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT 
TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG 
TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT 
CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT 
GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG 
GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT 
ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

en(am)-2 ( XM_011395198.1 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_011393500.1 Neurospora crassa OR74A glutamate synthase, variant (en(am)-2), mRNA.

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CloneID	ONf04205
Clone ID Related Accession (Same CDS sequence)	XM_011395197.1 , XM_011395198.1
Accession Version	XM_011395198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	glutamate synthase, variant
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026502).

ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG 
AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC 
CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC 
ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG 
ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT 
CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG 
CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA 
TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT 
TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA 
ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG 
AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC 
AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT 
TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC 
ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA 
AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT 
GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT 
GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG 
GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG 
GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT 
ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC 
CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC 
GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT 
GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG 
GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG 
CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG 
GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC 
ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT 
CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC 
AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT 
CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC 
CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG 
TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT 
AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC 
ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT 
GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC 
GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT 
GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT 
ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT 
GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC 
CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT 
GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC 
GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG 
TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC 
CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC 
GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT 
CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT 
GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC 
AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA 
CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT 
GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC 
AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT 
CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG 
ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT 
TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT 
AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT 
GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG 
GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC 
CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT 
CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT 
ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC 
AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC 
ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC 
AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC 
GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC 
AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT 
GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA 
TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC 
CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC 
GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT 
GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC 
GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC 
ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC 
GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC 
ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC 
ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT 
CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC 
AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC 
AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG 
AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT 
AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT 
CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG 
TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT 
GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT 
GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC 
TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC 
ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC 
GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC 
ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC 
GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC 
ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC 
CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA 
AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC 
CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT 
CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT 
ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG 
TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG 
TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC 
ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC 
GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT 
TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG 
TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT 
CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT 
GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG 
GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT 
ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011393500.1
CDS	483..6833
Translation	MGAIEDFDDRQVHLETEKEQYHPYEYQTENNSSWAGALPVKQGLYDPELEKDACGVGFACHIKGKPSHKIVSDARNLLCNMTHRGAVGSDARDGDGAGVMTSIPHKFFVKNFARDVGVTLPPLGQYAVGNLFFKPDPETLQESKRQLEEIAESLGLRVLGWREPPVDSTLLGPAALSREPSILQPFVVLASAYGPGNEPETTDPEKFDERLFERQLYVLRKRATHTIGLQNWFYICSLSNKNIVYKGQLAPIQVYQYYNDLVNYDYEAHFALVHSRFSTNTFPSWDRAQPLRWAAHNGEINTLRGNKNWMRAREGVMQSDVFGDELELLYPIVEAGGSDSAAFDNVLELLTINGVLSLPEAVMLMVPEAWQGNQSMDPKKAAFYEWAACQMEPWDGPALFTFADGRFCGANLDRNGLRPCRYYVMDDDRIICASEVGTIPVEPEKIIQKGRLQPGRMLLVDTRAGRIIDDSELKAAVSSREDFRSWLDNELITMPQVLETLSGSQSIELAAKPDNTKIQEDPLLHAFGYTFEQVSLLLGPMASDEKEALGSMGNDAPLACLSDAPKLLYEYFRQLFAQVTNPPIDPIRESIVMSLECYVGPQGNLLEMDSSQCGRLLLPSPILSIPEFNALKNMSTIHPEWTVKTIDLTFPKKEGIEGYIRHLDYICMEATAAIEASDRIIVLSDRNTSKDRVAVSALLAGGMVHHHLVSNKWRAMAAIVAETAEAREVHHMCVLLGYGVDAINPYLAMECIIKLNKEKLIKKKLTDDELIYNYKHSCDGGILKVMSKMGISTLASYKGAQIFEALGVDDSVVERCFKGTASRIKGITFELIAEDAFRLHERGFPSRYTVAVPGLPESGEYHWRDNGEAHVNDPTSIANIQDAVRTKNDKSYEAYSLSEYEQIKSCTLRGMLDFNFEDCSPIPIDQVEPWTEIVRRFCTGAMSYGSISLESHSTLAVAMNRLGGKSNTGEGGEDPERSQRLPNGDTMRSAIKQVASGRFGVTSAYLADSDELQIKMAQGAKPGEGGELPGHKVSKSIARTRHSTPGVGLISPPPHHDIYSIEDLKQLIYDLKCSSPRSRVSVKLVSETGVGIVASGVAKAKADHILISGHDGGTGASRWTGIKYAGLPWELGLAETHQTLVLNDLRGRVVVQTDGQLRTGRDVAIACLLGAEEWGFATTPLIAMGCIMMRKCHLNTCPVGIATQDPELRKKFTGTPEHVINFFYYVANELRAIMARLGFRTINEMVGHVEVLKVREDLRTKKTSNIDLSLILTPAHKLRPGVATFNVRKQDHKLYVRLDNKLITEAELTLDKGLPSRIECDIVNTDRAMGTSLSYQISKRYGEKGLPDDTVHVNIKGSAGQSFGAFLAPGVTLELEGDANDYVGKGLSGGRLIVYPPRSAVFKAEENIIVGNVCLYGATSGTCFFRGVAAERFAVRNSGATAVVEGVGDHGCEYMTGGRVVILGSTGRNFAAGMSGGIAYILDIQGDFLTKLNTEMVEASTLEEAEPEEIAFIRSLVEDHHHYTGSELAARILVDFSRALKRFIKVLPVDYKRVLEEEKAKAVKQAAETKRAEYSLPAAARKEKDPHAPKLQDIEESITDNAISKKKALVLDKTRGFMKYQRRAEKYRKPATRVKDWAELSQRLDEDELKYQSARCMDCGVPFCQSDSGCPISNIIPKWNELVFQGQWRDALNRLLMTNNFPEFTGRVCPAPCEGACVLGINEQPVGIKSIECAIIDRGFEMGWMVPEPPKIRTGKNVAIIGSGPAGLAAADQLNKAGHNVTVYERADRLGGLLMYGIPNMKLDKRIVKRRTDFMAAEGVQFKTGVAIGEDITLVDLKKQNDAVIIATGATVARDLPIKNRNLEGIHFAMEFLHKNTKSLLDSELADGSYISAKDKHVIVIGGGDTGNDCIGTSVRHGAKSVINFELLPKPPPARGRDNPWPQWPRVYRVDYGHDEVKQHTGKDPREYCIMSEEFVDDGNGKVRGINTIRVEWTKSASGGWDMKKIEGSQQFFPADLVLLSMGFLGPEARVLGDEIEKDPRKNVKTPPGKYSTNVEGVFAAGDCRRGQSLIVWGINEGRQAAREVDLYLEKSTCLPVTGGIVKRTAEEVFAAAAAA

Target ORF information:

RefSeq Version	XM_011395198.1
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A glutamate synthase, variant (en(am)-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011395198.1

ORF Insert Sequence:

ATGGGCGCTA TCGAGGATTT CGACGACCGA CAAGTGCATC TTGAAACAGA GAAAGAGCAG 
TATCATCCCT ATGAATACCA AACAGAGAAT AACTCTTCGT GGGCTGGCGC CTTGCCGGTG 
AAGCAGGGAC TGTACGATCC CGAGCTCGAA AAGGATGCCT GTGGTGTAGG TTTTGCTTGC 
CATATCAAGG GCAAGCCCAG TCACAAGATT GTCAGCGATG CACGAAACCT GCTCTGCAAC 
ATGACCCATC GTGGTGCCGT GGGCTCTGAC GCACGCGATG GCGATGGTGC GGGTGTCATG 
ACATCGATCC CCCACAAGTT CTTCGTGAAG AACTTCGCGC GCGATGTGGG AGTTACGCTT 
CCTCCACTCG GCCAGTATGC TGTGGGAAAC TTGTTCTTCA AACCCGATCC AGAGACACTG 
CAGGAGTCGA AGCGGCAGTT GGAAGAGATT GCCGAGTCGT TGGGCTTGAG AGTTCTAGGA 
TGGCGAGAGC CTCCCGTGGA TTCGACTTTG CTTGGCCCTG CTGCTCTTTC CCGAGAGCCT 
TCCATTCTGC AGCCTTTCGT TGTGCTAGCG TCTGCTTACG GGCCCGGCAA TGAACCAGAA 
ACAACCGATC CCGAGAAGTT TGACGAGAGG CTGTTTGAGC GGCAGCTCTA TGTCCTCCGG 
AAGAGAGCTA CACACACGAT TGGGCTGCAG AACTGGTTCT ACATCTGCTC CCTTTCCAAC 
AAGAACATTG TCTACAAGGG TCAGCTGGCG CCGATTCAGG TTTACCAATA CTACAATGAT 
TTGGTGAACT ATGACTATGA GGCTCACTTT GCCTTGGTGC ATTCGCGTTT CTCCACCAAC 
ACCTTCCCCT CTTGGGATCG TGCCCAGCCT CTCCGATGGG CTGCTCACAA CGGTGAAATA 
AACACCCTTC GCGGCAACAA GAATTGGATG CGTGCCCGTG AGGGAGTCAT GCAGTCTGAT 
GTCTTTGGAG ATGAGCTGGA ACTTCTCTAC CCTATTGTTG AGGCTGGCGG TTCCGACTCT 
GCTGCCTTCG ATAACGTGTT GGAGCTCCTC ACCATCAACG GCGTTCTCTC TCTTCCCGAG 
GCCGTGATGC TGATGGTGCC CGAGGCTTGG CAAGGAAACC AGAGCATGGA TCCCAAGAAG 
GCCGCCTTCT ATGAGTGGGC AGCTTGCCAA ATGGAGCCCT GGGATGGCCC TGCGCTCTTT 
ACGTTCGCTG ATGGTCGTTT TTGCGGTGCC AACTTGGATC GTAACGGTCT CAGACCTTGC 
CGTTATTATG TGATGGACGA CGATCGCATT ATCTGCGCGT CTGAGGTCGG CACTATCCCC 
GTTGAGCCCG AGAAGATCAT TCAGAAGGGC CGTCTCCAAC CTGGCCGCAT GCTTCTCGTT 
GATACTCGCG CCGGTCGCAT CATTGATGAT TCCGAGTTGA AGGCTGCTGT CTCTAGCCGG 
GAGGACTTCA GGTCATGGCT CGACAACGAA CTCATCACCA TGCCTCAGGT GCTCGAGACG 
CTTTCCGGGA GCCAGAGCAT TGAGCTTGCT GCCAAGCCTG ATAACACCAA GATCCAGGAG 
GACCCCCTGT TGCACGCTTT TGGATACACC TTTGAGCAGG TGAGCTTGCT GCTTGGCCCC 
ATGGCCTCGG ATGAAAAGGA GGCTCTTGGT TCCATGGGCA ACGATGCTCC GCTTGCCTGT 
CTGTCCGATG CTCCCAAGTT GCTGTACGAA TATTTCCGTC AGCTGTTCGC TCAGGTCACC 
AACCCGCCCA TCGATCCCAT CAGAGAGTCG ATTGTCATGT CTCTTGAATG CTACGTTGGT 
CCCCAGGGCA ACCTTCTCGA AATGGACTCG TCCCAATGTG GAAGACTCCT GCTTCCTAGC 
CCCATCCTCT CCATTCCTGA GTTCAATGCA CTTAAGAACA TGTCTACTAT CCATCCCGAG 
TGGACTGTCA AGACCATTGA CTTGACATTC CCCAAGAAGG AAGGCATTGA GGGCTACATT 
AGACACCTTG ACTATATTTG TATGGAGGCT ACTGCCGCTA TTGAAGCTAG TGATCGTATC 
ATTGTGCTGT CTGACCGCAA CACCTCCAAG GACCGTGTCG CTGTTTCTGC TCTCCTCGCT 
GGTGGCATGG TTCACCATCA CCTTGTCAGC AACAAGTGGC GTGCAATGGC AGCCATTGTC 
GCTGAGACGG CCGAGGCTCG TGAGGTTCAC CACATGTGCG TCCTCCTTGG CTACGGTGTT 
GACGCCATCA ATCCCTACCT TGCCATGGAG TGCATCATCA AGCTCAACAA GGAGAAGCTT 
ATCAAGAAGA AGCTGACTGA TGATGAGCTG ATCTACAACT ACAAGCACTC CTGTGATGGT 
GGTATCCTCA AGGTCATGAG CAAGATGGGT ATCTCTACCT TGGCCAGTTA CAAAGGTGCC 
CAGATCTTTG AGGCCTTGGG TGTCGATGAC AGCGTGGTTG AGCGCTGCTT CAAAGGTACT 
GCCTCCCGTA TCAAGGGTAT TACTTTTGAA CTTATCGCTG AGGATGCCTT CCGTTTGCAC 
GAGCGCGGTT TCCCGTCGCG TTATACCGTT GCTGTTCCTG GACTTCCCGA GTCTGGTGAG 
TACCACTGGC GTGACAATGG CGAGGCACAT GTCAACGATC CTACTTCTAT TGCAAACATC 
CAGGATGCCG TCCGTACCAA GAACGACAAG TCTTACGAGG CCTACTCACT CTCCGAGTAC 
GAGCAGATCA AATCCTGCAC GCTTCGTGGT ATGCTTGACT TCAATTTTGA AGACTGCTCT 
CCTATCCCTA TTGACCAGGT TGAGCCTTGG ACCGAGATTG TTCGCCGATT CTGCACTGGT 
GCTATGTCGT ATGGTTCTAT CTCCCTCGAA TCTCACTCTA CCCTGGCCGT TGCCATGAAC 
AGGCTTGGTG GCAAGTCCAA CACCGGTGAA GGTGGTGAGG ATCCTGAACG GTCCCAAAGA 
CTACCGAACG GCGATACCAT GCGCTCAGCC ATCAAACAGG TGGCCTCGGG TCGCTTTGGT 
GTTACATCTG CTTATCTGGC CGACTCTGAC GAACTTCAGA TCAAGATGGC ACAGGGTGCC 
AAGCCTGGTG AGGGTGGTGA GCTTCCTGGC CACAAGGTTT CCAAGTCCAT TGCCCGCACT 
CGTCACTCTA CCCCTGGCGT CGGCCTCATC TCGCCACCTC CTCATCACGA TATCTACTCG 
ATTGAAGACT TGAAGCAGCT CATTTATGAC CTTAAGTGCT CGAGTCCTCG TTCTCGCGTT 
TCCGTCAAGC TTGTGTCTGA GACTGGTGTT GGTATCGTCG CCTCGGGTGT CGCTAAGGCT 
AAGGCAGACC ACATTCTCAT CTCTGGTCAT GACGGTGGCA CTGGTGCCTC TCGCTGGACT 
GGTATCAAGT ACGCCGGTCT CCCCTGGGAG TTGGGTCTTG CTGAAACTCA TCAGACCTTG 
GTGCTTAACG ACCTGCGTGG GCGCGTTGTT GTTCAGACTG ATGGTCAGCT TCGTACCGGC 
CGTGATGTCG CCATTGCTTG CTTGCTGGGC GCTGAAGAAT GGGGGTTCGC GACCACTCCT 
CTCATTGCCA TGGGCTGCAT CATGATGCGC AAGTGCCATT TGAATACCTG CCCTGTTGGT 
ATTGCCACTC AGGACCCTGA ACTTCGCAAG AAGTTCACTG GTACGCCTGA GCATGTGATC 
AACTTCTTCT ACTACGTAGC GAACGAGCTC CGTGCTATCA TGGCCAGGCT TGGTTTCCGC 
ACCATCAATG AGATGGTTGG TCATGTTGAG GTGCTCAAGG TTCGTGAGGA TCTCAGGACC 
AAGAAGACCT CCAACATTGA CCTATCTCTC ATCTTGACTC CCGCCCATAA ACTCCGTCCC 
GGGGTGGCAA CTTTCAATGT CCGCAAGCAG GATCACAAGC TTTACGTTCG CTTGGACAAC 
AAGCTTATTA CTGAGGCTGA GTTGACCCTT GATAAGGGAC TGCCTTCACG TATTGAGTGT 
GATATCGTTA ACACTGACCG CGCCATGGGC ACTTCACTGT CCTACCAAAT TTCCAAACGA 
TATGGCGAGA AGGGACTGCC CGATGACACT GTCCATGTCA ATATTAAGGG TTCTGCGGGC 
CAGTCCTTCG GTGCTTTCCT TGCTCCGGGT GTCACACTCG AATTGGAGGG CGATGCCAAC 
GATTACGTTG GCAAGGGTTT GTCCGGTGGT AGACTGATTG TCTACCCCCC TCGCTCCGCT 
GTATTCAAGG CTGAGGAGAA CATTATCGTT GGTAACGTGT GCTTGTACGG AGCCACTAGC 
GGTACCTGCT TCTTCCGCGG CGTTGCAGCT GAGCGTTTCG CTGTACGCAA CTCTGGTGCC 
ACCGCCGTTG TCGAGGGTGT TGGTGATCAC GGCTGCGAGT ACATGACGGG TGGTCGGGTC 
GTGATCCTGG GAAGTACTGG CCGTAACTTT GCTGCTGGTA TGTCTGGTGG AATCGCCTAC 
ATCTTGGATA TTCAGGGCGA CTTCTTGACC AAGCTCAACA CCGAGATGGT TGAAGCCAGC 
ACTCTTGAGG AGGCGGAGCC TGAAGAGATC GCATTCATCC GCAGTCTCGT TGAGGATCAT 
CACCACTACA CTGGTTCCGA GCTTGCTGCT CGTATTCTGG TTGACTTTTC CCGTGCCCTC 
AAGCGCTTCA TCAAGGTGCT ACCCGTCGAC TACAAGCGCG TACTGGAGGA GGAGAAGGCC 
AAGGCTGTCA AGCAGGCCGC CGAGACCAAG CGGGCTGAGT ACAGCCTTCC TGCGGCTGCG 
AGAAAGGAGA AGGATCCTCA TGCGCCCAAG CTTCAGGATA TTGAGGAGAG CATTACCGAT 
AACGCTATCA GCAAGAAGAA GGCCCTTGTA TTGGACAAGA CGCGCGGTTT TATGAAGTAT 
CAGCGTCGTG CTGAGAAGTA CCGGAAGCCC GCGACTCGTG TCAAGGATTG GGCTGAACTG 
TCTCAGCGTC TTGATGAGGA CGAACTCAAG TACCAGTCTG CCCGTTGCAT GGACTGCGGT 
GTTCCCTTCT GCCAATCCGA TAGCGGCTGC CCAATTTCCA ACATTATCCC CAAGTGGAAT 
GAGCTGGTCT TCCAGGGTCA GTGGCGAGAT GCATTGAACC GCCTGTTAAT GACCAACAAC 
TTCCCCGAGT TTACTGGTCG CGTTTGCCCT GCTCCTTGCG AGGGTGCTTG CGTCTTGGGC 
ATCAACGAGC AGCCTGTTGG AATCAAGTCG ATCGAGTGCG CCATCATTGA CCGTGGTTTC 
GAGATGGGAT GGATGGTACC GGAGCCCCCT AAGATTCGCA CAGGCAAGAA CGTTGCCATC 
ATCGGATCTG GTCCCGCTGG TCTCGCTGCC GCCGACCAGC TGAACAAGGC TGGTCACAAC 
GTTACTGTGT ATGAGCGCGC CGACCGTCTT GGTGGTCTGC TCATGTATGG CATTCCCAAC 
ATGAAATTGG ACAAGCGCAT CGTCAAGAGA CGTACTGATT TCATGGCTGC TGAGGGTGTC 
CAGTTCAAGA CTGGCGTTGC GATTGGCGAG GATATAACAC TCGTGGATCT CAAGAAGCAA 
AATGATGCTG TCATCATTGC TACCGGTGCT ACTGTCGCCC GCGACCTTCC TATCAAGAAC 
CGCAATCTTG AGGGCATTCA TTTCGCTATG GAGTTCCTGC ACAAGAACAC CAAGTCTCTT 
CTTGATTCCG AACTGGCCGA CGGTTCCTAC ATCTCTGCCA AGGATAAGCA TGTCATTGTT 
ATTGGTGGTG GTGATACCGG TAACGACTGC ATTGGTACCT CTGTCCGCCA CGGTGCCAAG 
TCGGTCATCA ACTTTGAGCT TCTCCCCAAG CCTCCCCCGG CTCGCGGCCG CGACAACCCG 
TGGCCGCAGT GGCCCCGCGT GTACCGCGTG GACTACGGCC ACGATGAGGT CAAGCAACAC 
ACGGGCAAGG ATCCTCGTGA ATACTGCATC ATGTCGGAGG AGTTTGTCGA TGACGGCAAC 
GGAAAGGTGA GGGGCATCAA CACGATCCGC GTGGAGTGGA CCAAGTCTGC CAGCGGCGGT 
TGGGACATGA AGAAGATCGA GGGCTCGCAG CAGTTCTTCC CTGCCGATCT TGTCCTGCTG 
TCCATGGGCT TTTTGGGACC TGAGGCTCGT GTGCTCGGCG ATGAAATCGA GAAGGATCCT 
CGCAAGAACG TAAAGACTCC TCCCGGCAAG TATAGCACCA ACGTCGAGGG CGTGTTCGCT 
GCCGGTGATT GCCGTCGGGG ACAGTCGCTC ATCGTCTGGG GTATCAACGA AGGTCGCCAG 
GCTGCTCGCG AGGTGGATCT CTACCTCGAG AAGAGCACAT GTTTGCCTGT TACTGGCGGT 
ATCGTCAAGC GCACCGCTGA GGAGGTGTTC GCCGCTGCCG CCGCAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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en(am)-2 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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