bni1 cDNA ORF clone, Neurospora crassa OR74A

Gene
Clones
gRNAs

Summary

Gene Symbol	bni1
Entrez Gene ID	3872138
Full Name	bni1-like
Gene Type	protein-coding
Organism	Neurospora crassa OR74A

mRNA and Protein(s)

mRNA	Protein	Name
XM_950898.2	XP_955991.1	cytokinesis protein sepA

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CP, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B
Nature422(6934)859-68(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following bni1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bni1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONf05111	XM_950898.2 Latest version!	Neurospora crassa OR74A cytokinesis protein sepA (bni1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bni1 ( XM_950898.2 ) cDNA ORF clone, Neurospora crassa OR74A -> XP_955991.1 Neurospora crassa OR74A cytokinesis protein sepA (bni1), mRNA.

Price & Availability↑

CloneID	ONf05111
Clone ID Related Accession (Same CDS sequence)	XM_950898.2
Accession Version	XM_950898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5454bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-10
Organism	Neurospora crassa OR74A
Product	cytokinesis protein sepA
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_026505). On Feb 23, 2015 this sequence version replaced XM_950898.1.

ATGTCCTCCC ACGACAAGAA TGGGAGGGCT ACCTCCTCGA GCGGCAAGGC CTCCTCCTTC 
TTCTCGAGAG CCACTGGCAA GAACAAGGCC GACAAACGGA ACGCAAGCGA TGAGGGTGGC 
CGATACCTTG CCGCCGACAA CGACGCCGCC AGCACCCATA CCGCACGCAG TTCGCGCCAT 
TACCGGGACT CGTCTGTAAT ATCGCTGGAC CGTCCCGACA CGCCCGAATC TGGCCTCAAC 
ATGACGGCAG GGGTCGTCAC GACGATCCCC TATGACCCTG TTTCCCCCGA CAATCGCTCC 
CCCGTCGCCC TCGACCACCT TCCCAGGCCC GACCAAATGC CCCTAAGGCG AGAGCTAGTA 
CCGCACCACC TCAACAAAGG CACTGGTGAC TTCCACCAGT ATCCCACCAT CGACCAAAGC 
CCAAGCTCCC CCTATAATGG GCAGTCACCC AGCGCATCAA GTTCGCGCTC AACTCTCGCT 
ACTGCTAACA TCACCATGGC CTCCACGGGC CGCCAGGCCC AGTTTCAGCA ATGGGGACCA 
CCGAGAGAGA GCAGTTTGCC CGGGTACAAT TCCAGAGGCC ACTCGTACAT GACAACCCGC 
AGTTCGGGCG ACAATGCCAG CATCTACTCA GGTAGGGACG GACACTACCA AGAAGCCCCA 
GGAACCAGCC ATCGCTCATC CAGGATAGGA CTCCCTAGTA CTTCGTCTCA GTCATCCTAC 
CTTTCTCCCC ATGCCCATAA GGACAGAGAT ACCCAGCGGT TAACCAAGGT CGCTGCCGGC 
CACCAGGGCT CCGAAGGATT CAACTTTCCC AAGCCAGACG ATGACAATGT GATTGAACAG 
ATGTTCATAA ACCTGATGCA AAAGCGAGGA TGGCACAACC TCCCGGACCA GGCCAAGCGC 
CAGATGATCG CATACCCCGC CGCGAAGAAA TGGACCTTGA TTTACCAGGA TCGACTCACC 
GAGTGGCAGG GCGAACAGAA GCGCAGACAG ACGGCCAAGA TTGGCCAATA CAGCAATGTC 
GATATTACCC AATCCTCCGA TGACGAAGGC AGCCCCGAGT GGTATGTGCG CAAGGTCATG 
GAAAACGCTC TGGACAGCAA AGGGCTCGGA GGACTCGAAG TCAACCTTCG GACACAGCAG 
ATCGGCTGGG TCAAGCGCTT CATCGACTGT CAGGGACAGA TCGCCTTGAC CAATGTCCTG 
CTAAAGATGA ACCGAAAGAC TGCCTATGGA CCTGCTCAGC CGGATAATGG AAGATTGGAT 
AAGAATTTGG ACCGAGAATA TGACATTGTC AAATGTTTGA AGGCTCTTAT GAACAACAAG 
TTTGGCGCAG ACGATGCGCT AGCTCACCAA CAGGTTATTG TGGCTCTGGC CACCTCCCTT 
ATATCACCTC GGCTCACCAC CCGGAAGCTT GTCAGCGAAG TCCTGACATT CCTCTGCCAT 
TGGGGCGAAG GCAAGGGCCA TGTCAAGGTC ATCGAGGCCA TGGACGTCGT TAAGGCCCAG 
CAAGGCGAAA ACGGGCGCTT CGATGCTTGG ATGCGTCTTG TTGAGGTCAC CGTCGACGGC 
CGCGGGAAGA TGGGCAGTTT GGTCGGTGCT AGTGAGGAGG TACGAAGTGG TGGCATTGGC 
ATGGAGAATC TGCTCATGGA ATACGCCGTT GCTACCCTCA TCTTGGTCAA CATGTTGGTC 
GATGCCCCTG AGAAGGACCT GCAGCTGCGC ATACACATTC GGGCTCAGTT TACGGCCTGT 
GGCATCAGGC GTATCCTGAA CAAGATGGAG GCCTTTCAGT ATGAGCTGAT TGACAAGCAA 
ATCGAGCGGT TCCGCTCCAA CGAAGCTATC GACTATGAGG ATATGCTCGA GCGTGAGAAC 
AGCAGCATCA AAGATAGTAT CGAGGGTGAG GTTAAGGACC TCAACGACCC GGTACAAATT 
GTAGATGCTA TACAGCAGCG GCTTAATGGA ACCAAGACAC AGGACTACTT TGTATCTGCT 
CTCCAGCATT TGCTGTTGAT CAGGGACAAT GATGGCGAGG AGAGATTACG CATGTTCCAG 
CTGGTGGACT CTATGCTCAG CTACGTAGCT ATGGACCGCC GGTTGCCGGA CATGGACCTC 
AAGCAGAGTC TCAACTTCAC CGTTCAAAGT CTGTTAGATA AGCTCCATAC CGACTCTGAA 
GCACGTCAAG CTCTAGATGA GGCCCTCGAA GCCCGGCAGA TAGCTGATGC CGCTATGGCG 
GAGAGGGATG AGATGAAGGA GAGACTGGCC TTGGGAGCTG ATGGTCTGGT GGCTAAGCTA 
CAAAAGCAGA TTGATGAGCA AGCCATGTTC ATCGAGGCCC AGAAAAGGCG TGCTGAGGGT 
CTCAAGTCGG AGCTTGCCAG TTTGCAGACC CTGCGGGCAA AGGAGGCGCA ACGCTACGAG 
CTAGAGACCC GCGAACTGTA CCTCATGCTT CGCGACGCTC AAGATGTAGC TGCCTCCAAT 
GCCACCAAGG GCAACGCTAA GCTTGGTGAG GAAGACGTGG CCCGCATGCA AGGCATCCTC 
GATCGCGAGA GGCTTCTGGA GCGCCTGCAA ATGCAGATCG AGCGCCAAAA GACGCAGTTC 
AAGCTTGAAG GAAGAGTATG GGGCGAAGTG ATCGGCCCTT CGGATAGGCT CCGAGCTCTT 
CGGGAGGAGA TGGATGGCTT TGGCGACGAC ACACCGGAGG TTGTGCCACC CAAGGACTTC 
ACCAACAGCA TGCTTGGTAG CGTCAAGAGG TCGACCAGAA TACCCAGAAA GCCTGTTGGT 
TCCCGGTCAG ACCATGTCGA GGACGTCCTA GAGGAGAGCG ATGTCGAGAT TGGGGACGAC 
GGCGAGATCG TGTACGAGAA GCCTCGCGTG GTCAAGATCA GGAGGCCTGT CGTGGACCCC 
AAGCAGACCA ATGGTATGTT CAACGAACTC ACTGGGAAGG TCAGAAGGTA CGATGCCAGC 
GACTCCGAGG AAGGAGATGG TATCACAACC GGTCCCTCAC ATCCCAGCAT GGAGTCCCAG 
ACACCCATCA CACCCAGCGA TACCGAGACT CCCAAAATTC AAGTTACCGA TGCTACCGGA 
CCGTCTGCGC CAGCCGCGGC ATCAGGCCCT CCACCTCCAC CACCCCCGCC GCCTCCGCCT 
CCGCCGCCCC CTGGCTTCCT CCCTGGCGCC CCAGCTCCGA TTCCCGGCGC GGGCGGGCCT 
CCTCCGCCGC CGCCGCCTCC TCCTCCTCCT CCGCCGCCGC CAGGAGGCCT TCCGGGTGCT 
GCTCCTCCGA TGCCTGGCGC TGGTGGTCCA CCGCCGCCGC CGCCGCCACC ACCTCCTCCG 
CCAATGCCTG GTATGGCTGG TATGCCACCA CCTCCACCTC CGCCCCCTCC CATGCCTGGT 
ATGCCTGGTA TGCCACCACC ACCACCTCCT CCCATGCCAG GTCCTGCTTC GGGCCGCTTC 
TTGGCACAGA ACGCCGGGCT TCCCACGACC AATTCTGTTG GGCTACCTGT CGTCCGGCCC 
AAGAAGAAGC TCAAGGCTTT CCATTGGGAC AAGGTCGACA CGCCACTGGC AACGCACTGG 
GCGGCACATA CACCGTCCCC TGAGTCGAGG GAAGAAAAGT ATCATGAGCT CAGCAAGAAG 
GGTATTCTGG ACGAAGTTGA GAAGTTGTTC ATGGCGAAGG AGATCAAGAA GCTTGGCGGT 
GGAGGTGCCA AGAAGGATGA CAAGAAGCAG ATTATCTCCA ACGATTTGCG CAAGGCATTC 
GAAATCGCGC TTGCCAAATT CTCACAGTTC CCTGTTGAGA AGGTGGTGCA GATGATCATT 
CACTGCGACA AAGACGTGCT CGACAACCCG GTCGTCATGG ATTTCCTGCA GAAGGATGAT 
CTTTGCAACA TTTCGGATAA CACTGCCAAA GTTATGGCAC CATATAGCAA GGACTGGACT 
GGACCAGATG CCGGCAAAGA TACCCGAGAA CAGGATCCTA ACGAGCTCAC AAGACAGGAT 
CAGATATATC TGTATACGGC GTTCGAACTT CATCACTACT GGAAGAGTAG GATGAGGGCC 
CTTGCACTGA CTCGCAGCTT CGAACAAGAA TACGAGGAGA TTACTGAAAA GATGCGCGCC 
GTTGTAGCAG TGTCCGAATC TTTGAGAGAT TCCGTGTCCC TTATGAACGT ACTCGGCCTC 
ATCTTGGATA TCGGTAACTA TATGAACGAT GCGAACAAGC AAGCCCGCGG TTTCAAACTC 
TCTTCTCTTG CCAGACTCGG CATGGTGAAG GACGACAAGA ACGAGTCGAC GCTTGCGGAT 
CTCGTCGAAC GTATTGTGCG CCAGCAATAC CCAGAGTGGG AAGATTTCGC CAAGGACATC 
GAGGGCGTTA TTGCGGCCCA GAAGATCAAC ATCGAACAGC TTCAGGCTGA TGCCAAGAGG 
TATATCGACA ACATCAAGAA CGTGCAAATG TCTCTTGACT CAGGCAATCT CTCCGATCCG 
AAGAAGTTCC ATCCCCAGGA TCGCGTGTCG CAGATTGTGC AGAGGGTCAT GAAGGATGCT 
CGGTTCAAGG CAGAGCAAAT GCAGTTGTAT CTGGAAGAGA TGGTGCGCAC ATACAACGAC 
ATCATGGTCT TTTACGGCGA AGACCCGTCA GACGACAATG CTCGTCGCGA ATTCTTTTCC 
AAGCTGGCGT CATTCATCAC GGAGTGGAAG AGGTCGCGCG AGAAGAACAT TCAACTGGAA 
GAGCAGCGTA AGCGGAACGA GGCATCGATG AAACGCAAGA ATGCCCAGTT CAAGGCTTTG 
CAAGCGAGCG AGGCACAAGG ACCGGCAAGC CCAACGAGTA CGGGGGCGAT GGACAGCTTA 
CTCGAGAAGC TCAGAGCGGC TGCTCCGCAA ACCAGAGACC AAAGAGACAG GAGGAGGAGA 
GCACGTCTCA AGGACCGACA TCAAGTCCGC ATCGCTTCAG GCCAACAAGT ACCAGATTTC 
AACGAAATCC CCGAGGTGGA AGCTGCCCTC CAAACTGGCA AGAGTGCTGC ATCCGCGACC 
ACGGACGACG ACGGCGCCGG CAACGGTCTT GTCAGCCCCG ATGCCCTACC GCCAACAAAG 
GAGGCTCCCG ATGCGGAGGA AGACGACGTC GCTCGACGCG CAGCTGCATT GTTGCAAAGC 
TTCCAGGGTG GAGACGGGGC GGAAGATGAT GCAGAAAAGA GGGAAAGTTT GAGGAAGTCG 
AGGAGACAGA CTGCGGCAGA GGAGAGGAAC ATGAGAAGGC GGAGGAGAGA AAAAGCCCAG 
ACTAGCACGG CTGGGGAGGA AGGGAAGGAC GAGTCAACAG CGGCAGATGG ATCAGAAGCC 
GCGCCTTCAG CTAGCGCTGA GGACCTTTCA GCTGCCGAAG CCAAGACGGA TGGGCCATCA 
TCACCACGAC CAGGCACAGC GACGGGTGCC GAGGCATCGG AGGAGACTGA GACTACACCT 
ACGGCGACGG CGACGGACAC GAATCCTGAA GAAAGTAAAG AAACAACAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_955991.1
CDS	926..6379
Translation	MSSHDKNGRATSSSGKASSFFSRATGKNKADKRNASDEGGRYLAADNDAASTHTARSSRHYRDSSVISLDRPDTPESGLNMTAGVVTTIPYDPVSPDNRSPVALDHLPRPDQMPLRRELVPHHLNKGTGDFHQYPTIDQSPSSPYNGQSPSASSSRSTLATANITMASTGRQAQFQQWGPPRESSLPGYNSRGHSYMTTRSSGDNASIYSGRDGHYQEAPGTSHRSSRIGLPSTSSQSSYLSPHAHKDRDTQRLTKVAAGHQGSEGFNFPKPDDDNVIEQMFINLMQKRGWHNLPDQAKRQMIAYPAAKKWTLIYQDRLTEWQGEQKRRQTAKIGQYSNVDITQSSDDEGSPEWYVRKVMENALDSKGLGGLEVNLRTQQIGWVKRFIDCQGQIALTNVLLKMNRKTAYGPAQPDNGRLDKNLDREYDIVKCLKALMNNKFGADDALAHQQVIVALATSLISPRLTTRKLVSEVLTFLCHWGEGKGHVKVIEAMDVVKAQQGENGRFDAWMRLVEVTVDGRGKMGSLVGASEEVRSGGIGMENLLMEYAVATLILVNMLVDAPEKDLQLRIHIRAQFTACGIRRILNKMEAFQYELIDKQIERFRSNEAIDYEDMLERENSSIKDSIEGEVKDLNDPVQIVDAIQQRLNGTKTQDYFVSALQHLLLIRDNDGEERLRMFQLVDSMLSYVAMDRRLPDMDLKQSLNFTVQSLLDKLHTDSEARQALDEALEARQIADAAMAERDEMKERLALGADGLVAKLQKQIDEQAMFIEAQKRRAEGLKSELASLQTLRAKEAQRYELETRELYLMLRDAQDVAASNATKGNAKLGEEDVARMQGILDRERLLERLQMQIERQKTQFKLEGRVWGEVIGPSDRLRALREEMDGFGDDTPEVVPPKDFTNSMLGSVKRSTRIPRKPVGSRSDHVEDVLEESDVEIGDDGEIVYEKPRVVKIRRPVVDPKQTNGMFNELTGKVRRYDASDSEEGDGITTGPSHPSMESQTPITPSDTETPKIQVTDATGPSAPAAASGPPPPPPPPPPPPPPPGFLPGAPAPIPGAGGPPPPPPPPPPPPPPPGGLPGAAPPMPGAGGPPPPPPPPPPPPMPGMAGMPPPPPPPPPMPGMPGMPPPPPPPMPGPASGRFLAQNAGLPTTNSVGLPVVRPKKKLKAFHWDKVDTPLATHWAAHTPSPESREEKYHELSKKGILDEVEKLFMAKEIKKLGGGGAKKDDKKQIISNDLRKAFEIALAKFSQFPVEKVVQMIIHCDKDVLDNPVVMDFLQKDDLCNISDNTAKVMAPYSKDWTGPDAGKDTREQDPNELTRQDQIYLYTAFELHHYWKSRMRALALTRSFEQEYEEITEKMRAVVAVSESLRDSVSLMNVLGLILDIGNYMNDANKQARGFKLSSLARLGMVKDDKNESTLADLVERIVRQQYPEWEDFAKDIEGVIAAQKINIEQLQADAKRYIDNIKNVQMSLDSGNLSDPKKFHPQDRVSQIVQRVMKDARFKAEQMQLYLEEMVRTYNDIMVFYGEDPSDDNARREFFSKLASFITEWKRSREKNIQLEEQRKRNEASMKRKNAQFKALQASEAQGPASPTSTGAMDSLLEKLRAAAPQTRDQRDRRRRARLKDRHQVRIASGQQVPDFNEIPEVEAALQTGKSAASATTDDDGAGNGLVSPDALPPTKEAPDAEEDDVARRAAALLQSFQGGDGAEDDAEKRESLRKSRRQTAAEERNMRRRRREKAQTSTAGEEGKDESTAADGSEAAPSASAEDLSAAEAKTDGPSSPRPGTATGAEASEETETTPTATATDTNPEESKETTG

Target ORF information:

RefSeq Version	XM_950898.2
Organism	Neurospora crassa OR74A
Definition	Neurospora crassa OR74A cytokinesis protein sepA (bni1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_950898.2

ORF Insert Sequence:

ATGTCCTCCC ACGACAAGAA TGGGAGGGCT ACCTCCTCGA GCGGCAAGGC CTCCTCCTTC 
TTCTCGAGAG CCACTGGCAA GAACAAGGCC GACAAACGGA ACGCAAGCGA TGAGGGTGGC 
CGATACCTTG CCGCCGACAA CGACGCCGCC AGCACCCATA CCGCACGCAG TTCGCGCCAT 
TACCGGGACT CGTCTGTAAT ATCGCTGGAC CGTCCCGACA CGCCCGAATC TGGCCTCAAC 
ATGACGGCAG GGGTCGTCAC GACGATCCCC TATGACCCTG TTTCCCCCGA CAATCGCTCC 
CCCGTCGCCC TCGACCACCT TCCCAGGCCC GACCAAATGC CCCTAAGGCG AGAGCTAGTA 
CCGCACCACC TCAACAAAGG CACTGGTGAC TTCCACCAGT ATCCCACCAT CGACCAAAGC 
CCAAGCTCCC CCTATAATGG GCAGTCACCC AGCGCATCAA GTTCGCGCTC AACTCTCGCT 
ACTGCTAACA TCACCATGGC CTCCACGGGC CGCCAGGCCC AGTTTCAGCA ATGGGGACCA 
CCGAGAGAGA GCAGTTTGCC CGGGTACAAT TCCAGAGGCC ACTCGTACAT GACAACCCGC 
AGTTCGGGCG ACAATGCCAG CATCTACTCA GGTAGGGACG GACACTACCA AGAAGCCCCA 
GGAACCAGCC ATCGCTCATC CAGGATAGGA CTCCCTAGTA CTTCGTCTCA GTCATCCTAC 
CTTTCTCCCC ATGCCCATAA GGACAGAGAT ACCCAGCGGT TAACCAAGGT CGCTGCCGGC 
CACCAGGGCT CCGAAGGATT CAACTTTCCC AAGCCAGACG ATGACAATGT GATTGAACAG 
ATGTTCATAA ACCTGATGCA AAAGCGAGGA TGGCACAACC TCCCGGACCA GGCCAAGCGC 
CAGATGATCG CATACCCCGC CGCGAAGAAA TGGACCTTGA TTTACCAGGA TCGACTCACC 
GAGTGGCAGG GCGAACAGAA GCGCAGACAG ACGGCCAAGA TTGGCCAATA CAGCAATGTC 
GATATTACCC AATCCTCCGA TGACGAAGGC AGCCCCGAGT GGTATGTGCG CAAGGTCATG 
GAAAACGCTC TGGACAGCAA AGGGCTCGGA GGACTCGAAG TCAACCTTCG GACACAGCAG 
ATCGGCTGGG TCAAGCGCTT CATCGACTGT CAGGGACAGA TCGCCTTGAC CAATGTCCTG 
CTAAAGATGA ACCGAAAGAC TGCCTATGGA CCTGCTCAGC CGGATAATGG AAGATTGGAT 
AAGAATTTGG ACCGAGAATA TGACATTGTC AAATGTTTGA AGGCTCTTAT GAACAACAAG 
TTTGGCGCAG ACGATGCGCT AGCTCACCAA CAGGTTATTG TGGCTCTGGC CACCTCCCTT 
ATATCACCTC GGCTCACCAC CCGGAAGCTT GTCAGCGAAG TCCTGACATT CCTCTGCCAT 
TGGGGCGAAG GCAAGGGCCA TGTCAAGGTC ATCGAGGCCA TGGACGTCGT TAAGGCCCAG 
CAAGGCGAAA ACGGGCGCTT CGATGCTTGG ATGCGTCTTG TTGAGGTCAC CGTCGACGGC 
CGCGGGAAGA TGGGCAGTTT GGTCGGTGCT AGTGAGGAGG TACGAAGTGG TGGCATTGGC 
ATGGAGAATC TGCTCATGGA ATACGCCGTT GCTACCCTCA TCTTGGTCAA CATGTTGGTC 
GATGCCCCTG AGAAGGACCT GCAGCTGCGC ATACACATTC GGGCTCAGTT TACGGCCTGT 
GGCATCAGGC GTATCCTGAA CAAGATGGAG GCCTTTCAGT ATGAGCTGAT TGACAAGCAA 
ATCGAGCGGT TCCGCTCCAA CGAAGCTATC GACTATGAGG ATATGCTCGA GCGTGAGAAC 
AGCAGCATCA AAGATAGTAT CGAGGGTGAG GTTAAGGACC TCAACGACCC GGTACAAATT 
GTAGATGCTA TACAGCAGCG GCTTAATGGA ACCAAGACAC AGGACTACTT TGTATCTGCT 
CTCCAGCATT TGCTGTTGAT CAGGGACAAT GATGGCGAGG AGAGATTACG CATGTTCCAG 
CTGGTGGACT CTATGCTCAG CTACGTAGCT ATGGACCGCC GGTTGCCGGA CATGGACCTC 
AAGCAGAGTC TCAACTTCAC CGTTCAAAGT CTGTTAGATA AGCTCCATAC CGACTCTGAA 
GCACGTCAAG CTCTAGATGA GGCCCTCGAA GCCCGGCAGA TAGCTGATGC CGCTATGGCG 
GAGAGGGATG AGATGAAGGA GAGACTGGCC TTGGGAGCTG ATGGTCTGGT GGCTAAGCTA 
CAAAAGCAGA TTGATGAGCA AGCCATGTTC ATCGAGGCCC AGAAAAGGCG TGCTGAGGGT 
CTCAAGTCGG AGCTTGCCAG TTTGCAGACC CTGCGGGCAA AGGAGGCGCA ACGCTACGAG 
CTAGAGACCC GCGAACTGTA CCTCATGCTT CGCGACGCTC AAGATGTAGC TGCCTCCAAT 
GCCACCAAGG GCAACGCTAA GCTTGGTGAG GAAGACGTGG CCCGCATGCA AGGCATCCTC 
GATCGCGAGA GGCTTCTGGA GCGCCTGCAA ATGCAGATCG AGCGCCAAAA GACGCAGTTC 
AAGCTTGAAG GAAGAGTATG GGGCGAAGTG ATCGGCCCTT CGGATAGGCT CCGAGCTCTT 
CGGGAGGAGA TGGATGGCTT TGGCGACGAC ACACCGGAGG TTGTGCCACC CAAGGACTTC 
ACCAACAGCA TGCTTGGTAG CGTCAAGAGG TCGACCAGAA TACCCAGAAA GCCTGTTGGT 
TCCCGGTCAG ACCATGTCGA GGACGTCCTA GAGGAGAGCG ATGTCGAGAT TGGGGACGAC 
GGCGAGATCG TGTACGAGAA GCCTCGCGTG GTCAAGATCA GGAGGCCTGT CGTGGACCCC 
AAGCAGACCA ATGGTATGTT CAACGAACTC ACTGGGAAGG TCAGAAGGTA CGATGCCAGC 
GACTCCGAGG AAGGAGATGG TATCACAACC GGTCCCTCAC ATCCCAGCAT GGAGTCCCAG 
ACACCCATCA CACCCAGCGA TACCGAGACT CCCAAAATTC AAGTTACCGA TGCTACCGGA 
CCGTCTGCGC CAGCCGCGGC ATCAGGCCCT CCACCTCCAC CACCCCCGCC GCCTCCGCCT 
CCGCCGCCCC CTGGCTTCCT CCCTGGCGCC CCAGCTCCGA TTCCCGGCGC GGGCGGGCCT 
CCTCCGCCGC CGCCGCCTCC TCCTCCTCCT CCGCCGCCGC CAGGAGGCCT TCCGGGTGCT 
GCTCCTCCGA TGCCTGGCGC TGGTGGTCCA CCGCCGCCGC CGCCGCCACC ACCTCCTCCG 
CCAATGCCTG GTATGGCTGG TATGCCACCA CCTCCACCTC CGCCCCCTCC CATGCCTGGT 
ATGCCTGGTA TGCCACCACC ACCACCTCCT CCCATGCCAG GTCCTGCTTC GGGCCGCTTC 
TTGGCACAGA ACGCCGGGCT TCCCACGACC AATTCTGTTG GGCTACCTGT CGTCCGGCCC 
AAGAAGAAGC TCAAGGCTTT CCATTGGGAC AAGGTCGACA CGCCACTGGC AACGCACTGG 
GCGGCACATA CACCGTCCCC TGAGTCGAGG GAAGAAAAGT ATCATGAGCT CAGCAAGAAG 
GGTATTCTGG ACGAAGTTGA GAAGTTGTTC ATGGCGAAGG AGATCAAGAA GCTTGGCGGT 
GGAGGTGCCA AGAAGGATGA CAAGAAGCAG ATTATCTCCA ACGATTTGCG CAAGGCATTC 
GAAATCGCGC TTGCCAAATT CTCACAGTTC CCTGTTGAGA AGGTGGTGCA GATGATCATT 
CACTGCGACA AAGACGTGCT CGACAACCCG GTCGTCATGG ATTTCCTGCA GAAGGATGAT 
CTTTGCAACA TTTCGGATAA CACTGCCAAA GTTATGGCAC CATATAGCAA GGACTGGACT 
GGACCAGATG CCGGCAAAGA TACCCGAGAA CAGGATCCTA ACGAGCTCAC AAGACAGGAT 
CAGATATATC TGTATACGGC GTTCGAACTT CATCACTACT GGAAGAGTAG GATGAGGGCC 
CTTGCACTGA CTCGCAGCTT CGAACAAGAA TACGAGGAGA TTACTGAAAA GATGCGCGCC 
GTTGTAGCAG TGTCCGAATC TTTGAGAGAT TCCGTGTCCC TTATGAACGT ACTCGGCCTC 
ATCTTGGATA TCGGTAACTA TATGAACGAT GCGAACAAGC AAGCCCGCGG TTTCAAACTC 
TCTTCTCTTG CCAGACTCGG CATGGTGAAG GACGACAAGA ACGAGTCGAC GCTTGCGGAT 
CTCGTCGAAC GTATTGTGCG CCAGCAATAC CCAGAGTGGG AAGATTTCGC CAAGGACATC 
GAGGGCGTTA TTGCGGCCCA GAAGATCAAC ATCGAACAGC TTCAGGCTGA TGCCAAGAGG 
TATATCGACA ACATCAAGAA CGTGCAAATG TCTCTTGACT CAGGCAATCT CTCCGATCCG 
AAGAAGTTCC ATCCCCAGGA TCGCGTGTCG CAGATTGTGC AGAGGGTCAT GAAGGATGCT 
CGGTTCAAGG CAGAGCAAAT GCAGTTGTAT CTGGAAGAGA TGGTGCGCAC ATACAACGAC 
ATCATGGTCT TTTACGGCGA AGACCCGTCA GACGACAATG CTCGTCGCGA ATTCTTTTCC 
AAGCTGGCGT CATTCATCAC GGAGTGGAAG AGGTCGCGCG AGAAGAACAT TCAACTGGAA 
GAGCAGCGTA AGCGGAACGA GGCATCGATG AAACGCAAGA ATGCCCAGTT CAAGGCTTTG 
CAAGCGAGCG AGGCACAAGG ACCGGCAAGC CCAACGAGTA CGGGGGCGAT GGACAGCTTA 
CTCGAGAAGC TCAGAGCGGC TGCTCCGCAA ACCAGAGACC AAAGAGACAG GAGGAGGAGA 
GCACGTCTCA AGGACCGACA TCAAGTCCGC ATCGCTTCAG GCCAACAAGT ACCAGATTTC 
AACGAAATCC CCGAGGTGGA AGCTGCCCTC CAAACTGGCA AGAGTGCTGC ATCCGCGACC 
ACGGACGACG ACGGCGCCGG CAACGGTCTT GTCAGCCCCG ATGCCCTACC GCCAACAAAG 
GAGGCTCCCG ATGCGGAGGA AGACGACGTC GCTCGACGCG CAGCTGCATT GTTGCAAAGC 
TTCCAGGGTG GAGACGGGGC GGAAGATGAT GCAGAAAAGA GGGAAAGTTT GAGGAAGTCG 
AGGAGACAGA CTGCGGCAGA GGAGAGGAAC ATGAGAAGGC GGAGGAGAGA AAAAGCCCAG 
ACTAGCACGG CTGGGGAGGA AGGGAAGGAC GAGTCAACAG CGGCAGATGG ATCAGAAGCC 
GCGCCTTCAG CTAGCGCTGA GGACCTTTCA GCTGCCGAAG CCAAGACGGA TGGGCCATCA 
TCACCACGAC CAGGCACAGC GACGGGTGCC GAGGCATCGG AGGAGACTGA GACTACACCT 
ACGGCGACGG CGACGGACAC GAATCCTGAA GAAAGTAAAG AAACAACAGG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of the filamentous fungus Neurospora crassa.
Nature422(6934)859-68(2003 Apr)
Galagan JE,Calvo SE,Borkovich KA,Selker EU,Read ND,Jaffe D,FitzHugh W,Ma LJ,Smirnov S,Purcell S,Rehman B,Elkins T,Engels R,Wang S,Nielsen CB,Butler J,Endrizzi M,Qui D,Ianakiev P,Bell-Pedersen D,Nelson MA,Werner-Washburne M,Selitrennikoff CP,Kinsey JA,Braun EL,Zelter A,Schulte U,Kothe GO,Jedd G,Mewes W,Staben C,Marcotte E,Greenberg D,Roy A,Foley K,Naylor J,Stange-Thomann N,Barrett R,Gnerre S,Kamal M,Kamvysselis M,Mauceli E,Bielke C,Rudd S,Frishman D,Krystofova S,Rasmussen C,Metzenberg RL,Perkins DD,Kroken S,Cogoni C,Macino G,Catcheside D,Li W,Pratt RJ,Osmani SA,DeSouza CP,Glass L,Orbach MJ,Berglund JA,Voelker R,Yarden O,Plamann M,Seiler S,Dunlap J,Radford A,Aramayo R,Natvig DO,Alex LA,Mannhaupt G,Ebbole DJ,Freitag M,Paulsen I,Sachs MS,Lander ES,Nusbaum C,Birren B

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bni1 cDNA ORF clone, Neurospora crassa OR74A

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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