UBE2O cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2O
Entrez Gene ID	106987763
Full Name	ubiquitin conjugating enzyme E2 O
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027052258.1	XP_026908059.1	(E3-independent) E2 ubiquitin-conjugating enzyme
XM_015086065.1	XP_014941551.1	(E3-independent) E2 ubiquitin-conjugating enzyme

Pathways from BioSystems
Gene Ontology

The following UBE2O gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2O cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc56305	XM_015086065.1 Latest version!	Acinonyx jubatus ubiquitin conjugating enzyme E2O (UBE2O), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAc248810	XM_027052258.1 Latest version!	Acinonyx jubatus ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2O ( XM_015086065.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014941551.1 Acinonyx jubatus ubiquitin conjugating enzyme E2O (UBE2O), mRNA.

Price & Availability↑

CloneID	OAc56305
Clone ID Related Accession (Same CDS sequence)	XM_015086065.1
Accession Version	XM_015086065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015131784.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGCCGTACC TTTTGGGTTT GAAGCTCTTT CTGGTTCATA GCCAGAAGTT GGGGGGGAAC 
GCACCCCGTA ATGACATCAG AGGCCATTCT GTCACACCGT GTTCTCTTTC TCAGCTGAAG 
CTAGAGGACC GCTCTGTGGT GCCCCGGGAC GTGGTCCGGC ACATGCGATC CACCGACAGC 
CAGTGCGGCA CCGTGATCGA CGTCAGCATC GACTGTGCCG TCAAGCTCAT CGGCACCAAC 
TGCATCATCT ACCCTGTCAA CAGCAAGGAC CTGCAGCACA TCTGGCCCTT CATGTACGGG 
GACTACATTG CCTATGACTG CTGGCTGGGG GAGGGCTACG ACTTAAAGAA CCAGATCATC 
CTGAAGCTGT CCAACGGTGC CAGGTGCTCC ATGAACACAG AAGATGGCGC CAAGCTCTAT 
GACGTCTGTC CCCACGTCAG CGACTCTGGT CTCTTCTTTG ATGACTCCTA TGGCTTCTAC 
CCGGGCCAGG TGCTCATCGG CCCCGCCAAG ATCTTCTCTA GCGCCCAGTG GCTGTCTGGG 
GTCAAGCCTG TGCTCAGCAC CAAGAGCAAG TTCCGTGTGG TGGTGGAAGA GGTGCAGGTC 
GTAGAGCTAA AAGTCACATG GATTACCAAG AGTTTCTGTC CAGGGGGCAC GGACAGCGTC 
AGCCCCCCGC CCTCTGTCAT TACCCAGGAA AACCTAGGCA GGGTGAAGCG TCTCGGATGC 
TTTGACCATG CTCAGCGGCA GCTTGGGGAG CGCTGTCTGT ATGTCTTCCC AGCCAAGGTA 
GAGCCGGCCA AGATTGCCTG TGAATGTCCA GAAAAACACT GCGCCCAGGG GGAGGGCTCT 
ATGGCCAAGA AGGTGAAGCG CCTGTTGAAG AAGCAGGTTG TGAGGATCAT GTCCTGCTCC 
CCGGACACGC AGTGCTCCCG AGACCATTCC ATGGAGGACC CGGGAAAGAA AGGAGGGGCC 
AAAGCCAAGA GCGAAACGGG GTCGGGCAAC ACGGATGACA CCAGCTCGGT GACCTCCTCT 
GCTAGCTCCA CCACCTCCTC CCAGAGTGGC AGCGGCACAA GTAGAAAAAA GAGCATCCCC 
TTGTCTATCA AGAACTTGAA GCGGAAACAC AAGCGGAAGA AGAATAAAAT CACTCGTGAC 
TTTAAGCCGG GGGACAGGGT GGCGGTGGAG GTGGTGACCA CCATGACCTC GGCAGACGTG 
ATGTGGCAAG ACGGCTCCGT GGAGTGCAAC ATCCGCTCCA ACGACCTCTT TCCCGTGCAC 
CACCTGGACA ACAACGAATT CTGCCCCGGA GACTTTGTGG TGGACAAGCG AGTCCAGACC 
TGTCCAGACC CCGCCGTCTA CGGCGTGGTG CAGTCTGGGG ACCACGTGGG CCGCACCTGC 
ATGGTGAAGT GGTTCAAGCT GCGGCCCAGT GGCGATGACG TGGAGCTGAT TGGAGAAGAG 
GAGGACGTGA GCGTCTACGA CATTGCTGAC CACCCTGACT TCCGGTTCCG CACCACGGAC 
ATCGTCATCC GCATCGGCAA TACTGAGGAT GGAGCTCCTC ACAAGGAGGA TGAGCCATCA 
GTTGGCCAGG TGGCCCGTGT GGACGTCAGC AGCAAGGTGG AGGTGGTGTG GGCTGACAAC 
TCAAAGACCA TCATTCTGCC CCAGCACTTG TACAACATCG AGTCTGAGAT TGAGGAGTCG 
GACTATGACT CGGTGGAAGG CAGCACCAGT GGGGCGTCCT CGGATGAGTG GGAGGACGAC 
AGTGACAGCT GGGAGACGGA CAACGGGCTG GTGGAGGATG AGCACCCCAA GATAGAGGAG 
CCCCCCATCC CGCCCACTGA GCAGCCGGCA GCCCCTGAGG AGGACAAGGG GGCAGTGATC 
AGCGAAGAGG CAACCAGGCC GGGGGAGCGC CTTCTGACAG GCTCGCCCAC CTCTCCCACA 
GTGGAGCCGG AGAAACCGAC TCGGGAGAAG AAGTTTCTAG ATGACATCAA GAAACTGCAG 
GAAAATCTCA AGAAGACCCT GGACAATGTG GCCATCGCAG AGGAGGAGAA GATGGAGGCG 
ATGCCAGACA CGGAGCGCAA GGAGGAGAAG CCGGAAGGGC AGTCACCCGT GAAGGCCGAG 
TGGCCAAGCG AGACCCCGGT GCTCTGTCAG CAGTGCGGCG GCAAGCCCGG AGTCACCTTC 
ACCAGCGCTA AGGGCGAGGT CTTCTCAGTG CTGGAGTTCG CGCCCTCAAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCGGAAGCC AAGAAGTTCT TCAGCACAGT ACGGAAGGAG 
ATGGCGTTGC TGGCTACCTC GCTGCCTGAC GGCATCATGG TCAAGACCTT CGAGGACAGA 
ATGGCAAGTA GCCTGGCGTG GGCCTGGCGC GGGCGGCTGC AGACGTGCTC GTGTGGGTGC 
TTGGTACCCA GGGGGCTGCG GGACCCGGCA GCACCTGGGA GGCCGTCCGC ACCCCTCACG 
GGGCAGGAGC CCCGCACGTG TGTCCGGATC GCATGGTTCC TCCCTTTCTC CCTCCTGTTA 
AAAGTCAGTT TTGAAGCCCA GAGCTTTACC CACGCCCTGC TGGAGAGGGC CCAGGCGCTG 
CCCAACGGGA TCCCCAAGGA CAGCGGCTCC CCGGAGCCAC CCGCTGTGGC CGAGCTCTCA 
GACTACGGCC GAGAGGAAGC TGAGGACGGA GGGCTGTTCC CTGGAGAGGC CTCCCAGGGC 
TCAGACTCGG AGGGCGGCGC CCAGGGCCTG GCCTCGGCCA GCAGGGACCG CACAGACCAG 
ACTTCGGAGG CAGCGCCCGA CGCCCCGGTG CCCCCCAGTG TCAGACCAAA GAAGCGGAGA 
AAGAGCTACC GGAGCTTCTT GCCCGAGAAG AGCGGCTATC CTGACATCGG CTTCCCTCTC 
TTCCCACTCT CCAAGGGTTT CATCAAGAGC ATCCGGGGTG TCCTGACGCA GTTCCGGGCC 
GCTCTGCTAG AGGCGGGCAT GCCTGAGAGC GCGGAGGACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014941551.1
CDS	1..2985
Translation	MPYLLGLKLFLVHSQKLGGNAPRNDIRGHSVTPCSLSQLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVSIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGEGYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSAQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPGKKGGAKAKSETGSGNTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQTCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPTEQPAAPEEDKGAVISEEATRPGERLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAMPDTERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMASSLAWAWRGRLQTCSCGCLVPRGLRDPAAPGRPSAPLTGQEPRTCVRIAWFLPFSLLLKVSFEAQSFTHALLERAQALPNGIPKDSGSPEPPAVAELSDYGREEAEDGGLFPGEASQGSDSEGGAQGLASASRDRTDQTSEAAPDAPVPPSVRPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESAEDQ

Target ORF information:

RefSeq Version	XM_015086065.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus ubiquitin conjugating enzyme E2O (UBE2O), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015086065.1

ORF Insert Sequence:

ATGCCGTACC TTTTGGGTTT GAAGCTCTTT CTGGTTCATA GCCAGAAGTT GGGGGGGAAC 
GCACCCCGTA ATGACATCAG AGGCCATTCT GTCACACCGT GTTCTCTTTC TCAGCTGAAG 
CTAGAGGACC GCTCTGTGGT GCCCCGGGAC GTGGTCCGGC ACATGCGATC CACCGACAGC 
CAGTGCGGCA CCGTGATCGA CGTCAGCATC GACTGTGCCG TCAAGCTCAT CGGCACCAAC 
TGCATCATCT ACCCTGTCAA CAGCAAGGAC CTGCAGCACA TCTGGCCCTT CATGTACGGG 
GACTACATTG CCTATGACTG CTGGCTGGGG GAGGGCTACG ACTTAAAGAA CCAGATCATC 
CTGAAGCTGT CCAACGGTGC CAGGTGCTCC ATGAACACAG AAGATGGCGC CAAGCTCTAT 
GACGTCTGTC CCCACGTCAG CGACTCTGGT CTCTTCTTTG ATGACTCCTA TGGCTTCTAC 
CCGGGCCAGG TGCTCATCGG CCCCGCCAAG ATCTTCTCTA GCGCCCAGTG GCTGTCTGGG 
GTCAAGCCTG TGCTCAGCAC CAAGAGCAAG TTCCGTGTGG TGGTGGAAGA GGTGCAGGTC 
GTAGAGCTAA AAGTCACATG GATTACCAAG AGTTTCTGTC CAGGGGGCAC GGACAGCGTC 
AGCCCCCCGC CCTCTGTCAT TACCCAGGAA AACCTAGGCA GGGTGAAGCG TCTCGGATGC 
TTTGACCATG CTCAGCGGCA GCTTGGGGAG CGCTGTCTGT ATGTCTTCCC AGCCAAGGTA 
GAGCCGGCCA AGATTGCCTG TGAATGTCCA GAAAAACACT GCGCCCAGGG GGAGGGCTCT 
ATGGCCAAGA AGGTGAAGCG CCTGTTGAAG AAGCAGGTTG TGAGGATCAT GTCCTGCTCC 
CCGGACACGC AGTGCTCCCG AGACCATTCC ATGGAGGACC CGGGAAAGAA AGGAGGGGCC 
AAAGCCAAGA GCGAAACGGG GTCGGGCAAC ACGGATGACA CCAGCTCGGT GACCTCCTCT 
GCTAGCTCCA CCACCTCCTC CCAGAGTGGC AGCGGCACAA GTAGAAAAAA GAGCATCCCC 
TTGTCTATCA AGAACTTGAA GCGGAAACAC AAGCGGAAGA AGAATAAAAT CACTCGTGAC 
TTTAAGCCGG GGGACAGGGT GGCGGTGGAG GTGGTGACCA CCATGACCTC GGCAGACGTG 
ATGTGGCAAG ACGGCTCCGT GGAGTGCAAC ATCCGCTCCA ACGACCTCTT TCCCGTGCAC 
CACCTGGACA ACAACGAATT CTGCCCCGGA GACTTTGTGG TGGACAAGCG AGTCCAGACC 
TGTCCAGACC CCGCCGTCTA CGGCGTGGTG CAGTCTGGGG ACCACGTGGG CCGCACCTGC 
ATGGTGAAGT GGTTCAAGCT GCGGCCCAGT GGCGATGACG TGGAGCTGAT TGGAGAAGAG 
GAGGACGTGA GCGTCTACGA CATTGCTGAC CACCCTGACT TCCGGTTCCG CACCACGGAC 
ATCGTCATCC GCATCGGCAA TACTGAGGAT GGAGCTCCTC ACAAGGAGGA TGAGCCATCA 
GTTGGCCAGG TGGCCCGTGT GGACGTCAGC AGCAAGGTGG AGGTGGTGTG GGCTGACAAC 
TCAAAGACCA TCATTCTGCC CCAGCACTTG TACAACATCG AGTCTGAGAT TGAGGAGTCG 
GACTATGACT CGGTGGAAGG CAGCACCAGT GGGGCGTCCT CGGATGAGTG GGAGGACGAC 
AGTGACAGCT GGGAGACGGA CAACGGGCTG GTGGAGGATG AGCACCCCAA GATAGAGGAG 
CCCCCCATCC CGCCCACTGA GCAGCCGGCA GCCCCTGAGG AGGACAAGGG GGCAGTGATC 
AGCGAAGAGG CAACCAGGCC GGGGGAGCGC CTTCTGACAG GCTCGCCCAC CTCTCCCACA 
GTGGAGCCGG AGAAACCGAC TCGGGAGAAG AAGTTTCTAG ATGACATCAA GAAACTGCAG 
GAAAATCTCA AGAAGACCCT GGACAATGTG GCCATCGCAG AGGAGGAGAA GATGGAGGCG 
ATGCCAGACA CGGAGCGCAA GGAGGAGAAG CCGGAAGGGC AGTCACCCGT GAAGGCCGAG 
TGGCCAAGCG AGACCCCGGT GCTCTGTCAG CAGTGCGGCG GCAAGCCCGG AGTCACCTTC 
ACCAGCGCTA AGGGCGAGGT CTTCTCAGTG CTGGAGTTCG CGCCCTCAAA TCACTCTTTT 
AAGAAAATTG AGTTCCAGCC TCCGGAAGCC AAGAAGTTCT TCAGCACAGT ACGGAAGGAG 
ATGGCGTTGC TGGCTACCTC GCTGCCTGAC GGCATCATGG TCAAGACCTT CGAGGACAGA 
ATGGCAAGTA GCCTGGCGTG GGCCTGGCGC GGGCGGCTGC AGACGTGCTC GTGTGGGTGC 
TTGGTACCCA GGGGGCTGCG GGACCCGGCA GCACCTGGGA GGCCGTCCGC ACCCCTCACG 
GGGCAGGAGC CCCGCACGTG TGTCCGGATC GCATGGTTCC TCCCTTTCTC CCTCCTGTTA 
AAAGTCAGTT TTGAAGCCCA GAGCTTTACC CACGCCCTGC TGGAGAGGGC CCAGGCGCTG 
CCCAACGGGA TCCCCAAGGA CAGCGGCTCC CCGGAGCCAC CCGCTGTGGC CGAGCTCTCA 
GACTACGGCC GAGAGGAAGC TGAGGACGGA GGGCTGTTCC CTGGAGAGGC CTCCCAGGGC 
TCAGACTCGG AGGGCGGCGC CCAGGGCCTG GCCTCGGCCA GCAGGGACCG CACAGACCAG 
ACTTCGGAGG CAGCGCCCGA CGCCCCGGTG CCCCCCAGTG TCAGACCAAA GAAGCGGAGA 
AAGAGCTACC GGAGCTTCTT GCCCGAGAAG AGCGGCTATC CTGACATCGG CTTCCCTCTC 
TTCCCACTCT CCAAGGGTTT CATCAAGAGC ATCCGGGGTG TCCTGACGCA GTTCCGGGCC 
GCTCTGCTAG AGGCGGGCAT GCCTGAGAGC GCGGAGGACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2O ( XM_027052258.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026908059.1 Acinonyx jubatus ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

Price & Availability↑

CloneID	OAc248810
Clone ID Related Accession (Same CDS sequence)	XM_027052258.1
Accession Version	XM_027052258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	(E3-independent) E2 ubiquitin-conjugating enzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834836.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGACGGCAT CTCCAGCGTC CCCTAGCGAC GAGGCAGGAG CTCCTAGCAA CCGCATCTCG 
ATTCCCAGAC GACGGCGGCA CGTAGGGCCA CGTCAGCGCG CCGTGGCCCC GCCCCCGCGC 
CGGCTGGCCC CGCCTCCCCG TGCCGCGTTC GCCGCCGCCG CCGCCGCCGC GGGCACAGCA 
GTTACGGCGG ATCCCGCAGC GCCCGCGCCC GCAGCTCCGG CTCCCGCCCA GGCCCCGGCC 
GCGGTCGCGG TCGCGGCCCC GGACGCGGCG CCGACCCCGG ACGCGGCCCC CGCCCCGGCC 
CCGGCGCCGG CGCCCGCCCC GGACTCGGCC TCAGGGCCGT CCTCGGACTC CGGCCCCGAA 
GCCGGCTCGC AGCGGCTGCT CTTCTCTCAC GACCTGGTGT CGGGCCGTTA CCGCGGCTCG 
GTGCACTTCG GGCTGGTGCG CCTCATCCAC GGAGAGGACT CGGACTCGGA GGGCGAGGAG 
GAGGGCCGCG GGAGCTCGGG CTGCTCCGAG GCGGGGGGCG CGGGCCACGA GGAGGGCCGG 
GCCAGCCCGC TGCGCCGCGG CTACGTGCGC GTCCAGTGGT ACCCGGAGGG CGTCAAGCAG 
CACGTGAAGG AGACGAAGCT GAAGCTAGAG GACCGCTCTG TGGTGCCCCG GGACGTGGTC 
CGGCACATGC GATCCACCGA CAGCCAGTGC GGCACCGTGA TCGACGTCAG CATCGACTGT 
GCCGTCAAGC TCATCGGCAC CAACTGCATC ATCTACCCTG TCAACAGCAA GGACCTGCAG 
CACATCTGGC CCTTCATGTA CGGGGACTAC ATTGCCTATG ACTGCTGGCT GGGGAAGGTC 
TACGACTTAA AGAACCAGAT CATCCTGAAG CTGTCCAACG GTGCCAGGTG CTCCATGAAC 
ACAGAAGATG GCGCCAAGCT CTATGACGTC TGTCCCCACG TCAGCGACTC TGGTCTCTTC 
TTTGATGACT CCTATGGCTT CTACCCGGGC CAGGTGCTCA TCGGCCCCGC CAAGATCTTC 
TCTAGCGCCC AGTGGCTGTC TGGGGTCAAG CCTGTGCTCA GCACCAAGAG CAAGTTCCGT 
GTGGTGGTGG AAGAGGTGCA GGTCGTAGAG CTAAAAGTCA CATGGATTAC CAAGAGTTTC 
TGTCCAGGGG GCACGGACAG CGTCAGCCCC CCGCCCTCTG TCATTACCCA GGAAAACCTA 
GGCAGGGTGA AGCGTCTCGG ATGCTTTGAC CATGCTCAGC GGCAGCTTGG GGAGCGCTGT 
CTGTATGTCT TCCCAGCCAA GGTAGAGCCG GCCAAGATTG CCTGTGAATG TCCAGAAAAA 
CACTGCGCCC AGGGGGAGGG CTCTATGGCC AAGAAGGTGA AGCGCCTGTT GAAGAAGCAG 
GTTGTGAGGA TCATGTCCTG CTCCCCGGAC ACGCAGTGCT CCCGAGACCA TTCCATGGAG 
GACCCGGGAA AGAAAGGAGG GGCCAAAGCC AAGAGCGAAA CGGGGTCGGT CAGCCCCGAG 
GAGACGCCCG ATGGGGCCGC CAGCCCGGTG GAGATGCAGG ATGAGGGCCC AGAGGAGGCC 
CAGGACGCGG GTGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGGTGGCGA CGACAGGCTG 
CACTCGGCAG AGCAGGACGC GGACGACGAG GCCGCCGATG ACACGGATGA CACCAGCTCG 
GTGACCTCCT CTGCTAGCTC CACCACCTCC TCCCAGAGTG GCAGCGGCAC AAGTAGAAAA 
AAGAGCATCC CCTTGTCTAT CAAGAACTTG AAGCGGAAAC ACAAGCGGAA GAAGAATAAA 
ATCACTCGTG ACTTTAAGCC GGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACCATGACC 
TCGGCAGACG TGATGTGGCA AGACGGCTCC GTGGAGTGCA ACATCCGCTC CAACGACCTC 
TTTCCCGTGC ACCACCTGGA CAACAACGAA TTCTGCCCCG GAGACTTTGT GGTGGACAAG 
CGAGTCCAGA CCTGTCCAGA CCCCGCCGTC TACGGCGTGG TGCAGTCTGG GGACCACGTG 
GGCCGCACCT GCATGGTGAA GTGGTTCAAG CTGCGGCCCA GTGGCGATGA CGTGGAGCTG 
ATTGGAGAAG AGGAGGACGT GAGCGTCTAC GACATTGCTG ACCACCCTGA CTTCCGGTTC 
CGCACCACGG ACATCGTCAT CCGCATCGGC AATACTGAGG ATGGAGCTCC TCACAAGGAG 
GATGAGCCAT CAGTTGGCCA GGTGGCCCGT GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCAAAGAC CATCATTCTG CCCCAGCACT TGTACAACAT CGAGTCTGAG 
ATTGAGGAGT CGGACTATGA CTCGGTGGAA GGCAGCACCA GTGGGGCGTC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAACGGGC TGGTGGAGGA TGAGCACCCC 
AAGATAGAGG AGCCCCCCAT CCCGCCCACT GAGCAGCCGG CAGCCCCTGA GGAGGACAAG 
GGGGTAGTGA TCAGCGAAGA GGCTGCCACG GCCGCCATTC AGGGGGCCGT GGCCATGGCT 
GCCCCCGTGG CTGGGCTGAT GGAGAAGGCT GGCAAAGACG GGCCCCCGAA GAGCTTCCGG 
GAGCTGAAAG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACTGT GGAGCAGCTT 
CTGACAGGCT CGCCCACCTC TCCCACAGTG GAGCCGGAGA AACCGACTCG GGAGAAGAAG 
TTTCTAGATG ACATCAAGAA ACTGCAGGAA AATCTCAAGA AGACCCTGGA CAATGTGGCC 
ATCGCAGAGG AGGAGAAGAT GGAGGCGATG CCAGACACGG AGCGCAAGGA GGAGAAGCCG 
GAAGGGCAGT CACCCGTGAA GGCCGAGTGG CCAAGCGAGA CCCCGGTGCT CTGTCAGCAG 
TGCGGCGGCA AGCCCGGAGT CACCTTCACC AGCGCTAAGG GCGAGGTCTT CTCAGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAAATTGAGT TCCAGCCTCC GGAAGCCAAG 
AAGTTCTTCA GCACAGTACG GAAGGAGATG GCGTTGCTGG CTACCTCGCT GCCTGACGGC 
ATCATGGTCA AGACCTTCGA GGACAGAATG GACCTCTTCT CAGCCCTGAT CAAGGGCCCC 
ACTCGCACCC CCTACGAGGA CGGCCTCTAC TTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCAGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GTGGCCGCCT GAACCCCAAC 
CTGTATGACA ATGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATCGG AAAGGGCACA 
GAGAGGTGGA CAAGCAAATC CAGCCTCCTC CAGGTGCTCA TCTCCATCCA AGGTCTAATC 
CTGGTGAACG AACCGTACTA CAACGAGGCG GGCTTCGACA GCGACCGGGG CCTGCAGGAG 
GGGTATGAGA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGCGTGGT GCAGTCCATG 
ACCCAGTTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA ACACTTCAGC 
ACCGGGGGCT GGCGGCTGGT GAACCGCATC GAGTCCTGGC TGGAAACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGATC CCCAAGGACA GCGGCTCCCC GGAGCCACCC 
GCTGTGGCCG AGCTCTCAGA CTACGGCCGA GAGGAAGCTG AGGACGGAGG GCTGTTCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AGGGCCTGGC CTCGGCCAGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCCGACG CCCCGGTGCC CCCCAGTGTC 
AGACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CCGAGAAGAG CGGCTATCCT 
GACATCGGCT TCCCTCTCTT CCCACTCTCC AAGGGTTTCA TCAAGAGCAT CCGGGGTGTC 
CTGACGCAGT TCCGGGCCGC TCTGCTAGAG GCGGGCATGC CTGAGAGCGC GGAGGACCAG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026908059.1
CDS	1..4083
Translation	MTASPASPSDEAGAPSNRISIPRRRRHVGPRQRAVAPPPRRLAPPPRAAFAAAAAAAGTAVTADPAAPAPAAPAPAQAPAAVAVAAPDAAPTPDAAPAPAPAPAPAPDSASGPSSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVSIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSAQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIACECPEKHCAQGEGSMAKKVKRLLKKQVVRIMSCSPDTQCSRDHSMEDPGKKGGAKAKSETGSVSPEETPDGAASPVEMQDEGPEEAQDAGEQLPPFLLKEGGDDRLHSAEQDADDEAADDTDDTSSVTSSASSTTSSQSGSGTSRKKSIPLSIKNLKRKHKRKKNKITRDFKPGDRVAVEVVTTMTSADVMWQDGSVECNIRSNDLFPVHHLDNNEFCPGDFVVDKRVQTCPDPAVYGVVQSGDHVGRTCMVKWFKLRPSGDDVELIGEEEDVSVYDIADHPDFRFRTTDIVIRIGNTEDGAPHKEDEPSVGQVARVDVSSKVEVVWADNSKTIILPQHLYNIESEIEESDYDSVEGSTSGASSDEWEDDSDSWETDNGLVEDEHPKIEEPPIPPTEQPAAPEEDKGVVISEEAATAAIQGAVAMAAPVAGLMEKAGKDGPPKSFRELKEAIKILESLKNMTVEQLLTGSPTSPTVEPEKPTREKKFLDDIKKLQENLKKTLDNVAIAEEEKMEAMPDTERKEEKPEGQSPVKAEWPSETPVLCQQCGGKPGVTFTSAKGEVFSVLEFAPSNHSFKKIEFQPPEAKKFFSTVRKEMALLATSLPDGIMVKTFEDRMDLFSALIKGPTRTPYEDGLYLFDIQLPNIYPAVPPHFCYLSQCSGRLNPNLYDNGKVCVSLLGTWIGKGTERWTSKSSLLQVLISIQGLILVNEPYYNEAGFDSDRGLQEGYENSRCYNEMALIRVVQSMTQLVRRPPEVFEQEIRQHFSTGGWRLVNRIESWLETHALLERAQALPNGIPKDSGSPEPPAVAELSDYGREEAEDGGLFPGEASQGSDSEGGAQGLASASRDRTDQTSEAAPDAPVPPSVRPKKRRKSYRSFLPEKSGYPDIGFPLFPLSKGFIKSIRGVLTQFRAALLEAGMPESAEDQ

Target ORF information:

RefSeq Version	XM_027052258.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus ubiquitin conjugating enzyme E2 O (UBE2O), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027052258.1

ORF Insert Sequence:

ATGACGGCAT CTCCAGCGTC CCCTAGCGAC GAGGCAGGAG CTCCTAGCAA CCGCATCTCG 
ATTCCCAGAC GACGGCGGCA CGTAGGGCCA CGTCAGCGCG CCGTGGCCCC GCCCCCGCGC 
CGGCTGGCCC CGCCTCCCCG TGCCGCGTTC GCCGCCGCCG CCGCCGCCGC GGGCACAGCA 
GTTACGGCGG ATCCCGCAGC GCCCGCGCCC GCAGCTCCGG CTCCCGCCCA GGCCCCGGCC 
GCGGTCGCGG TCGCGGCCCC GGACGCGGCG CCGACCCCGG ACGCGGCCCC CGCCCCGGCC 
CCGGCGCCGG CGCCCGCCCC GGACTCGGCC TCAGGGCCGT CCTCGGACTC CGGCCCCGAA 
GCCGGCTCGC AGCGGCTGCT CTTCTCTCAC GACCTGGTGT CGGGCCGTTA CCGCGGCTCG 
GTGCACTTCG GGCTGGTGCG CCTCATCCAC GGAGAGGACT CGGACTCGGA GGGCGAGGAG 
GAGGGCCGCG GGAGCTCGGG CTGCTCCGAG GCGGGGGGCG CGGGCCACGA GGAGGGCCGG 
GCCAGCCCGC TGCGCCGCGG CTACGTGCGC GTCCAGTGGT ACCCGGAGGG CGTCAAGCAG 
CACGTGAAGG AGACGAAGCT GAAGCTAGAG GACCGCTCTG TGGTGCCCCG GGACGTGGTC 
CGGCACATGC GATCCACCGA CAGCCAGTGC GGCACCGTGA TCGACGTCAG CATCGACTGT 
GCCGTCAAGC TCATCGGCAC CAACTGCATC ATCTACCCTG TCAACAGCAA GGACCTGCAG 
CACATCTGGC CCTTCATGTA CGGGGACTAC ATTGCCTATG ACTGCTGGCT GGGGAAGGTC 
TACGACTTAA AGAACCAGAT CATCCTGAAG CTGTCCAACG GTGCCAGGTG CTCCATGAAC 
ACAGAAGATG GCGCCAAGCT CTATGACGTC TGTCCCCACG TCAGCGACTC TGGTCTCTTC 
TTTGATGACT CCTATGGCTT CTACCCGGGC CAGGTGCTCA TCGGCCCCGC CAAGATCTTC 
TCTAGCGCCC AGTGGCTGTC TGGGGTCAAG CCTGTGCTCA GCACCAAGAG CAAGTTCCGT 
GTGGTGGTGG AAGAGGTGCA GGTCGTAGAG CTAAAAGTCA CATGGATTAC CAAGAGTTTC 
TGTCCAGGGG GCACGGACAG CGTCAGCCCC CCGCCCTCTG TCATTACCCA GGAAAACCTA 
GGCAGGGTGA AGCGTCTCGG ATGCTTTGAC CATGCTCAGC GGCAGCTTGG GGAGCGCTGT 
CTGTATGTCT TCCCAGCCAA GGTAGAGCCG GCCAAGATTG CCTGTGAATG TCCAGAAAAA 
CACTGCGCCC AGGGGGAGGG CTCTATGGCC AAGAAGGTGA AGCGCCTGTT GAAGAAGCAG 
GTTGTGAGGA TCATGTCCTG CTCCCCGGAC ACGCAGTGCT CCCGAGACCA TTCCATGGAG 
GACCCGGGAA AGAAAGGAGG GGCCAAAGCC AAGAGCGAAA CGGGGTCGGT CAGCCCCGAG 
GAGACGCCCG ATGGGGCCGC CAGCCCGGTG GAGATGCAGG ATGAGGGCCC AGAGGAGGCC 
CAGGACGCGG GTGAGCAGCT GCCCCCCTTC CTGCTGAAGG AAGGTGGCGA CGACAGGCTG 
CACTCGGCAG AGCAGGACGC GGACGACGAG GCCGCCGATG ACACGGATGA CACCAGCTCG 
GTGACCTCCT CTGCTAGCTC CACCACCTCC TCCCAGAGTG GCAGCGGCAC AAGTAGAAAA 
AAGAGCATCC CCTTGTCTAT CAAGAACTTG AAGCGGAAAC ACAAGCGGAA GAAGAATAAA 
ATCACTCGTG ACTTTAAGCC GGGGGACAGG GTGGCGGTGG AGGTGGTGAC CACCATGACC 
TCGGCAGACG TGATGTGGCA AGACGGCTCC GTGGAGTGCA ACATCCGCTC CAACGACCTC 
TTTCCCGTGC ACCACCTGGA CAACAACGAA TTCTGCCCCG GAGACTTTGT GGTGGACAAG 
CGAGTCCAGA CCTGTCCAGA CCCCGCCGTC TACGGCGTGG TGCAGTCTGG GGACCACGTG 
GGCCGCACCT GCATGGTGAA GTGGTTCAAG CTGCGGCCCA GTGGCGATGA CGTGGAGCTG 
ATTGGAGAAG AGGAGGACGT GAGCGTCTAC GACATTGCTG ACCACCCTGA CTTCCGGTTC 
CGCACCACGG ACATCGTCAT CCGCATCGGC AATACTGAGG ATGGAGCTCC TCACAAGGAG 
GATGAGCCAT CAGTTGGCCA GGTGGCCCGT GTGGACGTCA GCAGCAAGGT GGAGGTGGTG 
TGGGCTGACA ACTCAAAGAC CATCATTCTG CCCCAGCACT TGTACAACAT CGAGTCTGAG 
ATTGAGGAGT CGGACTATGA CTCGGTGGAA GGCAGCACCA GTGGGGCGTC CTCGGATGAG 
TGGGAGGACG ACAGTGACAG CTGGGAGACG GACAACGGGC TGGTGGAGGA TGAGCACCCC 
AAGATAGAGG AGCCCCCCAT CCCGCCCACT GAGCAGCCGG CAGCCCCTGA GGAGGACAAG 
GGGGTAGTGA TCAGCGAAGA GGCTGCCACG GCCGCCATTC AGGGGGCCGT GGCCATGGCT 
GCCCCCGTGG CTGGGCTGAT GGAGAAGGCT GGCAAAGACG GGCCCCCGAA GAGCTTCCGG 
GAGCTGAAAG AGGCCATCAA GATCCTGGAG AGCCTCAAGA ACATGACTGT GGAGCAGCTT 
CTGACAGGCT CGCCCACCTC TCCCACAGTG GAGCCGGAGA AACCGACTCG GGAGAAGAAG 
TTTCTAGATG ACATCAAGAA ACTGCAGGAA AATCTCAAGA AGACCCTGGA CAATGTGGCC 
ATCGCAGAGG AGGAGAAGAT GGAGGCGATG CCAGACACGG AGCGCAAGGA GGAGAAGCCG 
GAAGGGCAGT CACCCGTGAA GGCCGAGTGG CCAAGCGAGA CCCCGGTGCT CTGTCAGCAG 
TGCGGCGGCA AGCCCGGAGT CACCTTCACC AGCGCTAAGG GCGAGGTCTT CTCAGTGCTG 
GAGTTCGCGC CCTCAAATCA CTCTTTTAAG AAAATTGAGT TCCAGCCTCC GGAAGCCAAG 
AAGTTCTTCA GCACAGTACG GAAGGAGATG GCGTTGCTGG CTACCTCGCT GCCTGACGGC 
ATCATGGTCA AGACCTTCGA GGACAGAATG GACCTCTTCT CAGCCCTGAT CAAGGGCCCC 
ACTCGCACCC CCTACGAGGA CGGCCTCTAC TTGTTTGACA TCCAGCTCCC CAACATCTAC 
CCAGCCGTGC CCCCCCACTT CTGCTACCTC TCCCAGTGCA GTGGCCGCCT GAACCCCAAC 
CTGTATGACA ATGGGAAGGT GTGCGTCAGC CTCCTGGGCA CCTGGATCGG AAAGGGCACA 
GAGAGGTGGA CAAGCAAATC CAGCCTCCTC CAGGTGCTCA TCTCCATCCA AGGTCTAATC 
CTGGTGAACG AACCGTACTA CAACGAGGCG GGCTTCGACA GCGACCGGGG CCTGCAGGAG 
GGGTATGAGA ACAGTCGCTG CTACAACGAG ATGGCGCTCA TCCGCGTGGT GCAGTCCATG 
ACCCAGTTGG TGCGGCGGCC GCCCGAGGTC TTCGAGCAGG AGATCCGGCA ACACTTCAGC 
ACCGGGGGCT GGCGGCTGGT GAACCGCATC GAGTCCTGGC TGGAAACCCA CGCCCTGCTG 
GAGAGGGCCC AGGCGCTGCC CAACGGGATC CCCAAGGACA GCGGCTCCCC GGAGCCACCC 
GCTGTGGCCG AGCTCTCAGA CTACGGCCGA GAGGAAGCTG AGGACGGAGG GCTGTTCCCT 
GGAGAGGCCT CCCAGGGCTC AGACTCGGAG GGCGGCGCCC AGGGCCTGGC CTCGGCCAGC 
AGGGACCGCA CAGACCAGAC TTCGGAGGCA GCGCCCGACG CCCCGGTGCC CCCCAGTGTC 
AGACCAAAGA AGCGGAGAAA GAGCTACCGG AGCTTCTTGC CCGAGAAGAG CGGCTATCCT 
GACATCGGCT TCCCTCTCTT CCCACTCTCC AAGGGTTTCA TCAAGAGCAT CCGGGGTGTC 
CTGACGCAGT TCCGGGCCGC TCTGCTAGAG GCGGGCATGC CTGAGAGCGC GGAGGACCAG 
TAG