GenCRISPR™ ゲノム編集関連製品 » GenCRISPR™ ヌクレアーゼ-CRISPR遺伝子編集 » GenCRISPR™ Ultra eSpCas9-2NLS-Research
GenCRISPR™ Ultra ESpCas9-2NLS-Research

A 20 μl reaction in 1×Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-2NLS for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

GenCRISPR™ Ultra eSpCas9-2NLS-Research

GenCRISPR™ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by expression in an E. coli strain carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less off-target activity than wild-type SpCas9 nuclease.
Z03622
¥138,657.00

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Description

_x000D_ _x000D_ The GenCRISPR™_x000D_ Ultra eSpCas9 product line provides customers with a selection of research use,_x000D_ basic GMP and GMP compliant Cas9 nucleases. The Cas9 protein can be formed with_x000D_ the guide RNA into a ribonucleoprotien (RNP) complex. The use of an RNP complex_x000D_ to perform gene editing has been shown to reduce the challenges encountered_x000D_ with other CRISPR gene editing techniques such as viral and plasmid delivery._x000D_ Challenges include off-target effects, cell viability and transcription/translational_x000D_ challenges.

GenCRISPR™_x000D_ Ultra eSpCas9-2NLS is a mutant form of Cas9 nuclease and is produced by_x000D_ expression in an E. coli strain_x000D_ carrying a plasmid encoding the eSpCas9 gene from Streptococcus pyogenes with double-ended nuclear localization_x000D_ signal (NLS). GenCRISPR™ Ultra eSpCas9-2NLS delivers higher fidelity and less_x000D_ off-target activity than wild-type SpCas9 nuclease._x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_ _x000D_

Note

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.

Overview
Key Features High knockout efficiencies: Consistent high performance in in-vitro plasmid cleavage test.
Tag-free: No extra tag amino acid
Animal-free: Plant-based culture media, Enzyme-free chromatographic purification.
DNA-free: No external DNA added to the system.

Applications
gRNA-dependent double-stranded DNA cleavage

Properties
Expression System Recombinant Cas9 with double-ended NLS expressed by E.coli
Species S. pyogenes
Tag Tag-free
Molecular Weight ~160 kDa
Concentration 10 mg/mL
Active temperature This eSpCas9 is active at 37°C.
Formulation Supplied as a solution of 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 50% Glycerol pH 8.0 at 25°C. 
Storage & Stability This product remains stable up to 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Quality Control Specifications
Appearance Clear, colorless liquid
Purity ≥ 95% as analyzed by SDS-PAGE
≥ 90% as analyzed by SEC-HPLC
Concentration by A280 10 mg/ml±1 mg/ml
Bioactivity (in vitro) ≥ 90%
Residual DNase Non-specific DNase activity
Residual RNase Non-specific RNase activity
Endotoxin Level ≤ 10 EU/mg as analyzed by gel clotting method

Examples
  • GenCRISPR™ Ultra ESpCas9-2NLS-Research
    • GenCRISPR™ Ultra ESpCas9-2NLS-Research

    A 20 μl reaction in 1×Cas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng GenCRISPR™ Ultra eSpCas9-2NLS for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.