一次抗体と二次抗体-抗-エピトープタグ、免疫測定、Cas 9 » Primary Antibodies » Immune Checkpoint Antibodies » Human TIGIT Antibody (20A9), mAb, Mouse
Human TIGIT Antibody (20A9), MAb, Mouse

Flow cytometric analysis of CHO-K1/TIGIT stable cell line (GenScript,M00542,Red) and CHO negative control cell (Black) binding with Human TIGIT Antibody (20A9), mAb, Mouse (GenScript, A01981-40)
Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG

Human TIGIT Antibody (20A9), mAb, Mouse

The product is specific for human TIGIT
A01981
¥19,506.00

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Overview
Specificity The product is specific for human TIGIT
Host Species Mouse
Immunogen Recombinant TIGIT protein
Conjugate Unconjugated

Applications
Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.01-0.05 µg/ml
Flow Cytometry 1- 5 µg/ml

Properties
Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.
Purification Protein A affinity column
Isotype Mouse IgG1
Clonality Monoclonal
Clone ID 20A9
Note GenScript can customize this product per customer's request including product size, buffer components, etc.

Examples
  • Human TIGIT Antibody (20A9), MAb, Mouse
    • Human TIGIT Antibody (20A9), MAb, Mouse

    Flow cytometric analysis of CHO-K1/TIGIT stable cell line (GenScript,M00542,Red) and CHO negative control cell (Black) binding with Human TIGIT Antibody (20A9), mAb, Mouse (GenScript, A01981-40)
    Antibody working concentration: 5 µg/ml, 2.5x105 cells/reaction
    The signal was developed with iFluor647 conjugated Goat Anti-Mouse IgG


Background
Target Background T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3, is a type I transmembrane protein, which belongs to the PVR family of immunoglobulin-like domain containing proteins. It is composed of an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on activated T cells, follicular T helper, memory T cells, and regulatory T cells and NK cells. TIGIT binds to CD155 (PVR) with high affinity and it regulates T-cell mediated immunity via the CD226/TIGIT-PVR pathway. TIGIT also serves as a negative regulator of NK and T cell activation.Human TIGIT Antibody (20A9), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with TIGIT.
Synonyms Mouse monoclonal to Human TIGIT/ VSIG9/ VSTM3/ WUCAM

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.