Cell line stable at expression level
One-stop solution from gene to assay
GenScript's stable cell line service covers all your needs in the aspects of protein/antibody production, and assay development. Not only have we developed cell lines stable at expression level, but also established stable cell lines ready for assay development. Furthermore, GenScript's CellPower™ platform guarantees over-expressing of gene of interest in any cell hosts.
Outline of Stable Cell Line Services
Fig. MTX amplification for CHO cells expressing a Fc fusion protein. The expression level of the Fc fusion protein increases more than 40-fold after 3 rounds of genomic amplification using MTX.
Subcloning of gene of interest
Membrane receptors: FACS, ELISA & Western Blot
Plasmid preparation
GPCRs: Ca2+, cAMP, IP-1, radiometric binding assays
Lentivirus packaging and production
Ion Channels: electrophysiology, Transporters: radiometric assay
Transduction & selection
Proteases, kinases, and other enzyme: substrate conversion assay
Q-PCR validated single(poly)-clone
Nuclear receptors: reporter gene assays
Deliverables: 2 vials of cells, 3X106 cells each vial
Cytokines: proliferation or other relevant assays
For our protein production mammalian stable cell line development service, please download .
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