DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THETM Anti DYKDDDDK (Flag) Tag Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG, such as anti mouse IgG antibodies and protein A.

ELISA; WB;

Mouse IgG2b,κ

Horseradish peroxidase (HRP)

A synthetic peptide (DYKDDDDK) coupled to KLH

DYKDDDDK (Flag) Tag Antibody Plate is a microtiter plate coated with THETM Anti DYKDDDDK (Flag) Tag Antibody. The plate is designed for the rapid capturing of DYKDDDDK (Flag) tagged protein expressed in different systems, including, E. coli, yeast and mammalian extracts and cell culture supernatants. It can be applied for the detection of DYKDDDDK (Flag) tag located at N-terminus, C-terminus, or internal of the protein. The plate demonstrates high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the DYKDDDDK (Flag) antibody plate than ones with higher M.W. The plate is tolerable with several common used reagents. Avoid using detection reagents that can recognize mouse IgG, such as anti mouse IgG antibodies and protein A.

WB; ICC/IF; IP;

Rabbit IgG

Unconjugated

Recombinant Protein of DYKDDDDK tag

ELISA; WB;

Mouse IgG2b,κ

Biotin

A synthetic peptide (DYKDDDDK) coupled to KLH

ELISA; WB; WB; ICC/IF; FCM;

Rabbit IgG

Unconjugated

A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH

GenScript Anti-DYKDDDDK IP Resin is designed for quick and efficient immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex. The resin is an agarose beads coupled to Mouse anti-DYKDDDDK monoclonal antibody (clone 6H8E4). The antibody recognizes N-terminal, internal and C-terminal fusion protein containing DYKDDDDK tag. It is supplied as a suspension in 50 % glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4,0.02 % (w/v) sodium azide (PBS/A).

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.