Recombinant Human Flt-3 Ligand Protein
Recombinant Human Flt-3 Ligand Protein

Measured in a cell proliferation assay using human OCI-AML5 cells. The expected ED50 is ≤ 1.0 ng/ml.
The specific activity of 5~20*10^5IU/mg，which is calibrated against the human FLT-3L WHO reference standard (NIBSC code: 96/532) .

Test ZO7020 NIBSC 96/532
ED50 (ng/mL) 0.443 0.638
Relative activity with NIBSC 144% 100%

Recombinant Human Flt-3 Ligand Protein

Human Flt-3 Ligand (FLT3L) is a hematopoietic growth factor that supports the proliferation and differentiation of hematopoietic progenitor cells, dendritic cells, and NK cells. This CHO-expressed recombinant protein provides high biological activity and low endotoxin levels for stem cell and immunology research.
Z07020
¥97,562.00

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Product Introduction
Species Human
Protein Construction
Flt-3L (Thr27-Pro184)
Accession # P49771
Purity ≥ 95.0% as analyzed by SDS-PAGE
≥ 95.0% as analyzed by HPLC
Endotoxin Level < 10.0 EU/mg
Biological Activity The ED50 as determined by the dose-dependent stimulation of the proliferation of human OCI-AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of 5~20*10^5IU/mg (NIBSC 96/532)
Expression System CHO
Apparent Molecular Weight 17.9 kDa.
Formulation 10mM PB, 50mM NaCl ,5% Trehalose and 0.01% Tween 80, pH 7.4
Reconstitution Reconstitute lyophilized protein with sterile pyrogen-free water to 0.25 mg/mL
Storage & Stability 24 months at -20±5°C

Examples
  • Recombinant Human Flt-3 Ligand Protein
    • Recombinant Human Flt-3 Ligand Protein

    Measured in a cell proliferation assay using human OCI-AML5 cells. The expected ED50 is ≤ 1.0 ng/ml.
    The specific activity of 5~20*10^5IU/mg，which is calibrated against the human FLT-3L WHO reference standard (NIBSC code: 96/532) .

    Test ZO7020 NIBSC 96/532
    ED50 (ng/mL) 0.443 0.638
    Relative activity with NIBSC 144% 100%


Background
Target Background Fms-related tyrosine kinase 3 ligand (Flt3L) is growth fator stimulates the proliferation and differentiation of hematopoietic multipotent progenitors and promotes proliferation of NK cells and dendritic cell subgroups by combination with other growth factors. Flt3L is produced by T cells and stromal fibroblasts, and targeted various cells including hematopoietic stem cells, B cells, T cells, dendritic cells, and NK cells. Flt3L binds to it cognate tyrosine kinase receptor Flt3 and activates JAK/STAT signaling pathway.Flt3L is a hematopoietic four helical bundle cytokine with structurally homologous to stem cell factor (SCF) and colony stimulating facor 1 (CSF-1) demonstrated four conserved cysteines and two glycosylation sites. Flt3L naturally as a non-disulfide-linked homodimer with multiple isoforms. The extracellular portion is approximately 160 amino acid residues in length and the cytoplasmic segment is approximately 20- 30 amino acid residues in length.
Synonyms Flt3 ligand; Flt3LG; SL cytokine; Fms-related tyrosine kinase 3 ligand.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.