Recombinant Human SCF Protein
Recombinant Human SCF Protein

Measured in a cell proliferation assay using Mo7e cells.
The ED50 for this effect is 5-8 ng/mL. The specific activity of 5~20*10^5IU/mg，which is calibrated against the human SCF WHO reference standard (NIBSC code: 91/682) .

Test ZO7019 NIBSC 96/532
ED50 (ng/mL) 6.16 7.79
Relative activity with NIBSC 126% 100%

Recombinant Human SCF Protein

Human SCF (Stem Cell Factor), also known as KIT Ligand, is a critical regulator of hematopoietic stem cell maintenance and proliferation through activation of the c-KIT receptor. This CHO-expressed recombinant protein offers high activity and low endotoxin levels for stem cell research and cell culture applications.
Z07019
¥59,134.00

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Product Introduction
Species Human
Protein Construction
SCF (Glu26-Ala190)
Accession # P21583
Purity ≥ 95.0% as analyzed by SDS-PAGE
≥ 95.0% as analyzed by HPLC
Endotoxin Level < 10.0 EU/mg
Biological Activity Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 5-8 ng/mL, corresponding to a specific activity of 5~20*10^5IU/mg (NIBSC 91/682)
Expression System CHO
Apparent Molecular Weight 18.5 kDa.
Formulation 10mM PB, 50mM NaCl, 5% Trehalose and 0.01% Tween 80, pH 7.4
Reconstitution Reconstitute lyophilized protein with sterile pyrogen-free water to 0.25 mg/mL
Storage & Stability 24 months at -20±5°C

Examples
  • Recombinant Human SCF Protein
    • Recombinant Human SCF Protein

    Measured in a cell proliferation assay using Mo7e cells.
    The ED50 for this effect is 5-8 ng/mL. The specific activity of 5~20*10^5IU/mg，which is calibrated against the human SCF WHO reference standard (NIBSC code: 91/682) .

    Test ZO7019 NIBSC 96/532
    ED50 (ng/mL) 6.16 7.79
    Relative activity with NIBSC 126% 100%


Background
Target Background Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. It stimulates the proliferation of myeloid, erythroid, and lymphoid progenitors in bone marrow cultures and has been shown to act synergistically with colony stimulating factors. SCF plays an important role in the hematopoiesis during embryonic development. SCF can regulates HSCs in the stem cell niche in the bone marrow. SCF has been shown to increase the survival of HSCs in vitro and contributes to the self-renewal and maintenance of HSCs in-vivo.
Synonyms Kit ligand; C-Kit Ligand; Mast Cell Growth Factor; MGF; Steel Factor; Stem cell factor.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.