Recombinant Human TPO Protein
Recombinant Human TPO Protein

Measured in a cell proliferation assay using Mo7e human leukemia cell line.
The ED50 for this effect is ≤1.0 ng/mL. The specific activity of ≥ 1 x 10^7 IU/mg，which is calibrated against the human TPO WHO reference standard (NIBSC code: 03/124).

Test ZO7018 NIB SC 03/124
ED50 (ng/mL) 0.44 1.52
Relative activity with NIBSC 345% 100%

Recombinant Human TPO Protein

Human TPO (Thrombopoietin) is a key hematopoietic cytokine that regulates megakaryocyte maturation and platelet production. This CHO-expressed recombinant protein exhibits high biological activity, low endotoxin levels, and is ideal for hematopoietic stem cell expansion and cell culture applications.
Z07018
¥110,433.00

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Product Introduction
Species Human
Protein Construction
SCF (Ser22-Leu195)
Accession # P40225
Purity ≥ 95.0% as analyzed by SDS-PAGE
≥ 95.0% as analyzed by HPLC
Endotoxin Level < 10.0 EU/mg
Biological Activity Measured in a cell proliferation assay using Mo7e human leukemia cell line. The ED50 for this effect is ≤ 1.0 ng/mL, corresponding to a specific activity of ≥ 1.0 x 10^7 IU/mg (NIBSC 03/124)
Expression System CHO
Apparent Molecular Weight 18.6 kDa.
Formulation 10 mM PB, 50 mM NaCl ,5% Trehalose and 0.01% Tween 80, pH 7.4
Reconstitution Reconstitute lyophilized protein with sterile pyrogen-free water to 0.25 mg/mL
Storage & Stability 24 months at -20±5°C

Examples
  • Recombinant Human TPO Protein
    • Recombinant Human TPO Protein

    Measured in a cell proliferation assay using Mo7e human leukemia cell line.
    The ED50 for this effect is ≤1.0 ng/mL. The specific activity of ≥ 1 x 10^7 IU/mg，which is calibrated against the human TPO WHO reference standard (NIBSC code: 03/124).

    Test ZO7018 NIB SC 03/124
    ED50 (ng/mL) 0.44 1.52
    Relative activity with NIBSC 345% 100%


Background
Target Background Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Synonyms Thrombopoietin; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growthand development factor; Myeloproliferative leukemia virus oncogene ligand; THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.