GARP&Latent TGF Beta 1 Complex, His & Avi, Rat
GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

Immobilized GARP&Latent TGF Beta 1 Complex, His & Avi, Rat, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.64 μg/ml determined by ELISA.

GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

The purity of GARP&Latent TGF Beta 1 Complex, His & Avi, Rat is greater than 95% as determined by SEC-HPLC.

GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

GARP&Latent TGF Beta 1 Complex, His & Avi, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Z06419
¥104,464.00

Ask us a question
Product Introduction
Species Rat
Protein Construction
GARP&Latent TGF Beta 1 Complex [Ile18-Asn628(GARP)&Leu30-Arg278(Latent TGF Beta 1)]
Accession # D3ZVD5(GARP)&P17246(Latent TGF Beta)		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • GARP&Latent TGF Beta 1 Complex, His & Avi, Rat
    • GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

    Immobilized GARP&Latent TGF Beta 1 Complex, His & Avi, Rat, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.64 μg/ml determined by ELISA.

  • GARP&Latent TGF Beta 1 Complex, His & Avi, Rat
    • GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

    The purity of GARP&Latent TGF Beta 1 Complex, His & Avi, Rat is greater than 95% as determined by SEC-HPLC.

  • GARP&Latent TGF Beta 1 Complex, His & Avi, Rat
    • GARP&Latent TGF Beta 1 Complex, His & Avi, Rat

    GARP&Latent TGF Beta 1 Complex, His & Avi, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background GARP&Latent TGF Beta is a complex found on surface of many types of cells. In Tregs, GARP is involved in TCR-mediated activation of Latent TGF-β and thus promoting secretion and activation of TGF-β. Integrin αvβ8 on the surface of immune cells and other cells recognizes RGD in LAP, resulting in the release of mature TGF-β from the TGF-β&GARP complex.
Synonyms LRRC32; GARP; LAP; TGF-beta-1; LRRC32&TGF-beta 1; LRRC32&TGFB1; TGF-β-1; LRRC32&TGF-β 1; GARP&Latent TGF Beta; GARP&Latent TGF Beta 1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.