SPP1/OPN, His, Mouse
SPP1/OPN, His, Mouse

The purity of SPP1/OPN, His, Mouse is greater than 95% as determined by SEC-HPLC.

SPP1/OPN, His, Mouse

SPP1/OPN, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SPP1/OPN, His, Mouse

Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.
Z06361
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Product Introduction
Species Mouse
Protein Construction
SPP1/OPN (Leu17-Asn294)_x000D_
Accession # P10923		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Expression System HEK293
Theoretical Molecular Weight 31.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • SPP1/OPN, His, Mouse
    • SPP1/OPN, His, Mouse

    The purity of SPP1/OPN, His, Mouse is greater than 95% as determined by SEC-HPLC.

  • SPP1/OPN, His, Mouse
    • SPP1/OPN, His, Mouse

    SPP1/OPN, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Secreted phosphoprotein 1 (SPP1) expression in TAMs isolated from lung adenocarcinoma tissues and PMA-treated THP-1 cells were measured. Macrophage polarization was identified by flow cytometric analysis. Cell migration and apoptosis were assessed by Transwell migration assays and flow cytometric analysis, respectively. SPP1 is highly expressed in tumor tissues and TAMs isolated from patients with an advanced TNM stage, and also in PMA-treated THP-1 cells.
Synonyms Osteopontin; Bone sialoprotein 1; Nephropontin; SPP-1; BNSP; OPN; SPP1; Uropontin; BSP-1; ETA-1; BSPI

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.