R spondin 1/RSPO1, His, Mouse
R Spondin 1/RSPO1, His, Mouse

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

R Spondin 1/RSPO1, His, Mouse

Immobilized R spondin 1/RSPO1, His, Mouse, His Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11 μg/ml determined by ELISA.

R Spondin 1/RSPO1, His, Mouse

R spondin 1/RSPO1, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

R spondin 1/RSPO1, His, Mouse

The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Z06333
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Product Introduction
Species Mouse
Protein Construction
R spondin 1/RSPO1 (Ser21-Gln265)_x000D_
Accession # Q9Z132		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 28.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 150mM NaCl, 200mM Arginine (pH 5.5).
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • R Spondin 1/RSPO1, His, Mouse
    • R Spondin 1/RSPO1, His, Mouse

    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 2 - 20 ng/mL in the presence of 5 ng/mL recombinant Human Wnt Surrogate-Fc Fusion (WNT-HM23A) (QC Test).

  • R Spondin 1/RSPO1, His, Mouse
    • R Spondin 1/RSPO1, His, Mouse

    Immobilized R spondin 1/RSPO1, His, Mouse, His Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for Mouse RNF43, hFc Tag with the EC50 of 0.11 μg/ml determined by ELISA.

  • R Spondin 1/RSPO1, His, Mouse
    • R Spondin 1/RSPO1, His, Mouse

    R spondin 1/RSPO1, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Synonyms R-spondin-1; RSPO1; CRISTIN3; FLJ40906; RP11-566C13.1; RSPO; R-Spondin1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.