Fc gamma RIII/CD16, His, Mouse
Fc Gamma RIII/CD16, His, Mouse

The purity of Fc gamma RIII/CD16, His, MouseI is greater than 95% as determined by SEC-HPLC.

Fc Gamma RIII/CD16, His, Mouse

Fc gamma RIII/CD16, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Fc Gamma RIII/CD16, His, Mouse

OKT3 captured on CM5 Chip via Protein A can bind Fc gamma RIII/CD16, His, Mouse, His Tag with an affinity constant of 2.34 μM as determined in SPR assay (Biacore T200).

Fc gamma RIII/CD16, His, Mouse

Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
Z06116
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Product Introduction
Species Mouse
Protein Construction
Fc gamma RIII/CD16 (Leu32-Thr215)
Accession # Q5D5I8		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity OKT3 captured on CM5 Chip via Protein A can bind Fc gamma RIII/CD16, His, Mouse in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 22.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-48 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from a 0.22 μm filtered solution in PBS, (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • Fc Gamma RIII/CD16, His, Mouse
    • Fc Gamma RIII/CD16, His, Mouse

    The purity of Fc gamma RIII/CD16, His, MouseI is greater than 95% as determined by SEC-HPLC.

  • Fc Gamma RIII/CD16, His, Mouse
    • Fc Gamma RIII/CD16, His, Mouse

    Fc gamma RIII/CD16, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • Fc Gamma RIII/CD16, His, Mouse
    • Fc Gamma RIII/CD16, His, Mouse

    OKT3 captured on CM5 Chip via Protein A can bind Fc gamma RIII/CD16, His, Mouse, His Tag with an affinity constant of 2.34 μM as determined in SPR assay (Biacore T200).


Background
Target Background Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
Synonyms IgG Fc receptor III; Fc-gamma RIII; FcRIII; FCGR3; CD16; CD16A; FCG3; FcgRIII; FCR-10; FCRIIIA; IGFR3; IMD20

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.