BID, His, Mouse
BID, His, Mouse

The purity of BID, His, Mouse is greater than 90% as determined by SEC-HPLC.

BID, His, Mouse

BID, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

BID, His, Mouse

BID, His, Mouse on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).

BID, His, Mouse

Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
Z06033
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Product Introduction
Species Mouse
Protein Construction
BID (Ala1-Ser195)_x000D_
Accession # EDK99650.1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 90% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Expression System E.coli
Theoretical Molecular Weight 22.72 kDa
Apparent Molecular Weight The protein has a predicted MW of 22.72 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • BID, His, Mouse
    • BID, His, Mouse

    The purity of BID, His, Mouse is greater than 90% as determined by SEC-HPLC.

  • BID, His, Mouse
    • BID, His, Mouse

    BID, His, Mouse on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • BID, His, Mouse
    • BID, His, Mouse

    BID, His, Mouse on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).


Background
Target Background Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
Synonyms p22 BID; BID;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.