Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is <30 ng/ml (QC Test).

Immobilized GDF-8, Human/Mouse/Rat, No Tag at 1 μg/ml(100 μI/well) on the plate. Dose response curve for Human/Cynomolgus Activin RIIB, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA.

Immobilized GDF-8, Human/Mouse/Rat, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Human Activin RIIA, hFc Tag with the EC50 of 56.9ng/ml determined by ELISA.

Immobilized GDF-8, Human/Mouse/Rat, No Tag at 2 μg/ml (100 μI/well) on the plate. Dose response curve for Anti-GDF8 Antibody, hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.

GDF-8, Human/Mouse/Rat on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 95%.

Human Activin RIIA, hFc Tag captured on CM5 Chip via Protein A can bind GDF-8, Human/Mouse/Rat, No Tag with an affinity constant of 0.13 nM as determined in SPR assay (Biacore T200).

Biotinylated Activin RIIB, His Tag captured on CM5 Chip via Streptavidin can bind GDF-8, Human/Mouse/Rat, No Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).

Anti-GDF8 Antibody, hFc-Avi Tag captured on CM5 Chip via Protein A can bind GDF-8, Human/Mouse/Rat, No Tag with an affinity constant of 49.45 pM as determined in SPR assay (Biacore T200).

GDF-8, Human/Mouse/Rat

Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.

Cat. No.	Z05970
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
TAT
Price	¥139,907.00


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Product Introduction

Species

Human/Mouse/Rat

Protein Construction

GDF-8 (Asp267-Ser375)_x000D_
Accession # O14793(Human) / O08689(Mouse) / O35312(Rat)

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

12.40 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22 μm filtered solution in 4 mM HCl.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 4 mM HCl.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples