SCF, Human
SCF, Human

Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 1-8 ng/mL (QC Test).

SCF, Human

Immobilized SCF, Human, No Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.71 μg/ml determined by ELISA.

SCF, Human

SCF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

SCF, Human

Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Z05798
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Product Introduction
Species Human
Protein Construction
SCF (Glu26-Ala189)
Accession # P21583-1
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 0.02EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. Measured in a cell proliferation assay using Mo7e cells. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 18.45 kDa
Apparent Molecular Weight The protein has a predicted MW of 18.45 kDa same as Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • SCF, Human
    • SCF, Human

    Measured in a cell proliferation assay using Mo7e cells. The ED50 for this effect is 1-8 ng/mL (QC Test).

  • SCF, Human
    • SCF, Human

    Immobilized SCF, Human, No Tag at 5 μg/ml (100 μI/well) on the plate. Dose response curve for Human CD117, His Tag with the EC50 of 1.71 μg/ml determined by ELISA.

  • SCF, Human
    • SCF, Human

    SCF, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Synonyms MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.