R spondin 3/RSPO3, His, Human
R Spondin 3/RSPO3, His, Human

Immobilized R spondin 3/RSPO3, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.

R Spondin 3/RSPO3, His, Human

Human R spondin 3/RSPO3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

R Spondin 3/RSPO3, His, Human

Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind R spondin 3/RSPO3, His, Human, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).

R spondin 3/RSPO3, His, Human

The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Z05783
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Product Introduction
Species Human
Protein Construction
R spondin 3/RSPO3 (Gln22-Val146)
Accession # Q9BXY4-1		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 14.95 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • R Spondin 3/RSPO3, His, Human
    • R Spondin 3/RSPO3, His, Human

    Immobilized R spondin 3/RSPO3, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA.

  • R Spondin 3/RSPO3, His, Human
    • R Spondin 3/RSPO3, His, Human

    Human R spondin 3/RSPO3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • R Spondin 3/RSPO3, His, Human
    • R Spondin 3/RSPO3, His, Human

    Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind R spondin 3/RSPO3, His, Human, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test).


Background
Target Background The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
Synonyms R-spondin-3; RSPO3; hPWTSR; hRspo3; PWTSR; THSD2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.