P-Selectin/CD62P, His, Human
P-Selectin/CD62P, His, Human

Immobilized P-Selectin/CD62P, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

P-Selectin/CD62P, His, Human

The purity of P-Selectin/CD62P, His, Human is greater than 95% as determined by SEC-HPLC.

P-Selectin/CD62P, His, Human

P-Selectin/CD62P, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

P-Selectin/CD62P, His, Human

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
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Product Introduction
Species Human
Protein Construction
P-Selectin/CD62P (Trp42-Ala771)_x000D_
Accession # P16109		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 81.05 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • P-Selectin/CD62P, His, Human
    • P-Selectin/CD62P, His, Human

    Immobilized P-Selectin/CD62P, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

  • P-Selectin/CD62P, His, Human
    • P-Selectin/CD62P, His, Human

    The purity of P-Selectin/CD62P, His, Human is greater than 95% as determined by SEC-HPLC.

  • P-Selectin/CD62P, His, Human
    • P-Selectin/CD62P, His, Human

    P-Selectin/CD62P, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Synonyms FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.