PD-L2/B7-DC, His & Avi, Human
PD-L2/B7-DC, His & Avi, Human

The purity of PD-L2/B7-DC, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

PD-L2/B7-DC, His & Avi, Human

PD-L2/B7-DC, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%

PD-L2/B7-DC, His & Avi, Human

Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind PD-L2/B7-DC, His & Avi, Human, His Tag with an affinity constant of 0.296 μM as determined in SPR assay (Biacore T200).

PD-L2/B7-DC, His & Avi, Human

PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
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Product Introduction
Species Human
Protein Construction
PD-L2/B7-DC (Leu20-Thr220)_x000D_
Accession # Q9BQ51		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Human PD­1, hFc Tag captured on CM5 Chip via Protein A can bind PD-L2/B7-DC, His & Avi, Human in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 25.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-52 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • PD-L2/B7-DC, His & Avi, Human
    • PD-L2/B7-DC, His & Avi, Human

    The purity of PD-L2/B7-DC, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

  • PD-L2/B7-DC, His & Avi, Human
    • PD-L2/B7-DC, His & Avi, Human

    PD-L2/B7-DC, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%

  • PD-L2/B7-DC, His & Avi, Human
    • PD-L2/B7-DC, His & Avi, Human

    Human PD-1, hFc Tag captured on CM5 Chip via Protein A can bind PD-L2/B7-DC, His & Avi, Human, His Tag with an affinity constant of 0.296 μM as determined in SPR assay (Biacore T200).


Background
Target Background PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Synonyms PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.