PD-L2/B7-DC hFc Chimera, Human
PD-L2/B7-DC HFc Chimera, Human

The purity of PD-L2/B7-DC hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

PD-L2/B7-DC HFc Chimera, Human

PD-L2/B7-DC hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

PD-L2/B7-DC HFc Chimera, Human

PD-L2/B7-DC hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).

PD-L2/B7-DC hFc Chimera, Human

PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Z05745
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Product Introduction
Species Human
Protein Construction
PD-L2/B7-DC (Leu20-Thr220)_x000D_
Accession # Q9BQ51		 hFc
N-term C-term
Purity > 95% as determined by Bis­-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Loaded PD-L2/B7-DC hFc Chimera, Human on ProA­-Biosensor can bind Human PD­1, His Tag in BLI assay (Gator® Prime). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 48.60 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • PD-L2/B7-DC HFc Chimera, Human
    • PD-L2/B7-DC HFc Chimera, Human

    The purity of PD-L2/B7-DC hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • PD-L2/B7-DC HFc Chimera, Human
    • PD-L2/B7-DC HFc Chimera, Human

    PD-L2/B7-DC hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • PD-L2/B7-DC HFc Chimera, Human
    • PD-L2/B7-DC HFc Chimera, Human

    PD-L2/B7-DC hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human PD-1, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).


Background
Target Background PD-1 ligand 2 (PD-L2) as a second ligand for PD-1 and compare the function and expression of PD-L1 and PD-L2. Engagement of PD-1 by PD-L2 dramatically inhibits T cell receptor (TCR)-mediated proliferation and cytokine production by CD4 T cells. At low antigen concentrations, PD-L2-PD-1 interactions inhibit strong B7-CD28 signals.
Synonyms PDL2; PD-L2; Butyrophilin B7-DC; CD273, PDCD1 ligand 2; PDCD1L2; PDCD1LG2; B7DC; bA574F11.2; Btdc

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.