PD-1/PDCD1, His, Human
PD-1/PDCD1, His, Human

Immobilized PD-1/PDCD1, His, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.

PD-1/PDCD1, His, Human

The purity of PD-1/PDCD1, His, Human is greater than 95% as determined by SEC-HPLC.

PD-1/PDCD1, His, Human

PD-1/PDCD1, His, Human on Bis-Tris PAGE under reduced. The purity is greater than 95%.

PD-1/PDCD1, His, Human

Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind PD-1/PDCD1, His, Human, His Tag with an affinity constant of 2.20 μM as determined in SPR assay (Biacore T200).

PD-1/PDCD1, His, Human

Human PD-L2, hFc Tag captured on CM5 Chip via Protein A can bind PD-1/PDCD1, His, Human, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).

PD-1/PDCD1, His, Human

Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Z05736
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Product Introduction
Species Human
Protein Construction
PD-1/PDCD1 (Leu25-Gln167)_x000D_
Accession # Q15116-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 17 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 38-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • PD-1/PDCD1, His, Human
    • PD-1/PDCD1, His, Human

    Immobilized PD-1/PDCD1, His, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-PD-1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.

  • PD-1/PDCD1, His, Human
    • PD-1/PDCD1, His, Human

    The purity of PD-1/PDCD1, His, Human is greater than 95% as determined by SEC-HPLC.

  • PD-1/PDCD1, His, Human
    • PD-1/PDCD1, His, Human

    PD-1/PDCD1, His, Human on Bis-Tris PAGE under reduced. The purity is greater than 95%.

  • PD-1/PDCD1, His, Human
    • PD-1/PDCD1, His, Human

    Human PD-L1, hFc Tag captured on CM5 Chip via Protein A can bind PD-1/PDCD1, His, Human, His Tag with an affinity constant of 2.20 μM as determined in SPR assay (Biacore T200).

  • PD-1/PDCD1, His, Human
    • PD-1/PDCD1, His, Human

    Human PD-L2, hFc Tag captured on CM5 Chip via Protein A can bind PD-1/PDCD1, His, Human, His Tag with an affinity constant of 0.21 μM as determined in SPR assay (Biacore T200).


Background
Target Background Programmed cell death protein 1, also known as PD-1 and CD279 , is a protein found on the surface of cells that has a role in regulating the immune system's response to the cells of the human body by down-regulating the immune system and promoting self tolerance by suppressing T cell inflammatory activity.
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.