PAI-1, His, Human

PAI-1, His, Human

PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.
Z05731
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Product Introduction
Species Human
Protein Construction
PAI-1 (Val24-Pro402)_x000D_
Accession # P05121-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its ability to inhibit uPA cleavage of a peptide substrate, N­carbobenzyloxy­Gly­Gly­Arg­7­amido­4­methylcoumarin (Z­GGR­AMC). The IC50 value is < 35 nM. Test result meets the standard.
Expression System HEK293
Theoretical Molecular Weight 43.86 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5).
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states.
Synonyms PAI; PAI-1; Serpin E1; SERPINE1; PAI1; PLANH1

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.