OX40/TNFRSF4/CD134, His & Avi, Human
OX40/TNFRSF4/CD134, His & Avi, Human

Immobilized OX40/TNFRSF4/CD134, His & Avi, Human, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

OX40/TNFRSF4/CD134, His & Avi, Human

The purity of OX40/TNFRSF4/CD134, His & Avi, Human is greater than 95% as determined by SEC HPLC.

OX40/TNFRSF4/CD134, His & Avi, Human

OX40/TNFRSF4/CD134, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

OX40/TNFRSF4/CD134, His & Avi, Human

OX40/TNFRSF4/CD134, His & Avi, Human, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).

OX40/TNFRSF4/CD134, His & Avi, Human

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
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Product Introduction
Species Human
Protein Construction
OX40/TNFRSF4/CD134 (Leu29-Ala216)_x000D_
Accession # P43489		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity OX40/TNFRSF4/CD134, His & Avi, Human captured on CM5 Chip via Anti­His Antibody can bind Human OX40 Ligand, hFc Tag in SPR assay (Biacore T200). Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 23.2 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • OX40/TNFRSF4/CD134, His & Avi, Human
    • OX40/TNFRSF4/CD134, His & Avi, Human

    Immobilized OX40/TNFRSF4/CD134, His & Avi, Human, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.

  • OX40/TNFRSF4/CD134, His & Avi, Human
    • OX40/TNFRSF4/CD134, His & Avi, Human

    The purity of OX40/TNFRSF4/CD134, His & Avi, Human is greater than 95% as determined by SEC HPLC.

  • OX40/TNFRSF4/CD134, His & Avi, Human
    • OX40/TNFRSF4/CD134, His & Avi, Human

    OX40/TNFRSF4/CD134, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • OX40/TNFRSF4/CD134, His & Avi, Human
    • OX40/TNFRSF4/CD134, His & Avi, Human

    OX40/TNFRSF4/CD134, His & Avi, Human, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).


Background
Target Background Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Synonyms CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue; TXGP1L; IMD16

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.