Nectin-3/CD113 hFc Chimera, Human
Nectin-3/CD113 HFc Chimera, Human

Immobilized Nectin-3/CD113 hFc Chimera, Human, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.11 μg/ml determined by ELISA.

Nectin-3/CD113 HFc Chimera, Human

The purity of Nectin-3/CD113 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

Nectin-3/CD113 HFc Chimera, Human

Nectin-3/CD113 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Nectin-3/CD113 hFc Chimera, Human

The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
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Product Introduction
Species Human
Protein Construction
Nectin-3/CD113 (Gly58-Asp400)_x000D_
Accession # Q9NQS3		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 63.63 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • Nectin-3/CD113 HFc Chimera, Human
    • Nectin-3/CD113 HFc Chimera, Human

    Immobilized Nectin-3/CD113 hFc Chimera, Human, hFc Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.11 μg/ml determined by ELISA.

  • Nectin-3/CD113 HFc Chimera, Human
    • Nectin-3/CD113 HFc Chimera, Human

    The purity of Nectin-3/CD113 hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • Nectin-3/CD113 HFc Chimera, Human
    • Nectin-3/CD113 HFc Chimera, Human

    Nectin-3/CD113 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
Synonyms CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.