Immobilized Nectin-2/CD112, His & Avi, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA.

The purity of Nectin-2/CD112, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Nectin-2/CD112, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Nectin-2/CD112, His & Avi, Human, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200).

Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Nectin-2/CD112, His & Avi, Human, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).

Nectin-2/CD112, His & Avi, Human

Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

Cat. No.	Z05673
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥55,963.00


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Product Introduction

Species

Human

Protein Construction

Nectin-2/CD112 (Gln32-Leu360)_x000D_ Accession # Q92692-2	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

38.4 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized Nectin-2/CD112, His & Avi, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA.

The purity of Nectin-2/CD112, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Nectin-2/CD112, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Nectin-2/CD112, His & Avi, Human, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200).

Background

Target Background	Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Synonyms	CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Nectin-2/CD112, His & Avi, Human

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