Immobilized Human Nectin-3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with the EC50 of 60.7ng/ml determined by ELISA.

Immobilized Human Nectin-4, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with the EC50 of 2.0μg/ml determined by ELISA.

The purity of Nectin-1/PVRL1/CD111, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Nectin-1/PVRL1/CD111, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).

Human Nectin-3, His Tag immobilized on CM5 Chip can bind Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200).

Nectin-1/PVRL1/CD111, His & Avi, Human

Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity.

Cat. No.	Z05670
Size	100 μg : Quote 500 μg : Quote Buy in bulk
Quantity
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Price	¥55,963.00


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Product Introduction

Species

Human

Protein Construction

Nectin-1/PVRL1/CD111 (Gln31-Thr334)_x000D_ Accession # Q15223-1	His	Avi
N-term		C-term

Purity

> 95% as determined by BisTris PAGE

Endotoxin Level

Less than 1EU per μg by the LAL method.

Biological Activity

Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.

Expression System

HEK293

Theoretical Molecular Weight

36.9 kDa

Apparent Molecular Weight

Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Storage & Stability

Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples

Immobilized Human Nectin-3, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with the EC50 of 60.7ng/ml determined by ELISA.

Immobilized Human Nectin-4, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with the EC50 of 2.0μg/ml determined by ELISA.

The purity of Nectin-1/PVRL1/CD111, His & Avi, Human is greater than 95% as determined by SEC-HPLC.

Nectin-1/PVRL1/CD111, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200).

Human Nectin-3, His Tag immobilized on CM5 Chip can bind Nectin-1/PVRL1/CD111, His & Avi, Human, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200).

Background

Target Background

Synonyms

CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

Nectin-1/PVRL1/CD111, His & Avi, Human

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