MICA hFc Chimera, Human
MICA HFc Chimera, Human

Immobilized Human NKG2D, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for MICA hFc Chimera, Human, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

MICA HFc Chimera, Human

Immobilized MICA hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45 μg/ml determined by ELISA.

MICA HFc Chimera, Human

The purity of MICA hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

MICA HFc Chimera, Human

MICA hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

MICA HFc Chimera, Human

MICA hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.36 μM as determined in SPR assay (Biacore T200).

MICA hFc Chimera, Human

MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
Z05648
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Product Introduction
Species Human
Protein Construction
MICA (Glu24-Gln308)
Accession # Q96QC4		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 59.64 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 75-85 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • MICA HFc Chimera, Human
    • MICA HFc Chimera, Human

    Immobilized Human NKG2D, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for MICA hFc Chimera, Human, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.

  • MICA HFc Chimera, Human
    • MICA HFc Chimera, Human

    Immobilized MICA hFc Chimera, Human, hFc Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Anti-MICA Antibody, hFc Tag with the EC50 of 0.45 μg/ml determined by ELISA.

  • MICA HFc Chimera, Human
    • MICA HFc Chimera, Human

    The purity of MICA hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • MICA HFc Chimera, Human
    • MICA HFc Chimera, Human

    MICA hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • MICA HFc Chimera, Human
    • MICA HFc Chimera, Human

    MICA hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 0.36 μM as determined in SPR assay (Biacore T200).


Background
Target Background MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
Synonyms FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.