Latent GDF-8, His, Human

Latent GDF-8, His, Human

Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Z05564
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Product Introduction
Species Human
Protein Construction
His Latent GDF-8 (Asn24-Ser375)_x000D_
Accession # O14793
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 1 EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Latent GDF-8, His, Human at 1 μg/ml (100 μl/well) on the plate can bind Anti­GDF8 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 41.19 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-40 kDa and 45-55 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background Growth/differentiation factor 8 (GDF8), or myostatin, negatively regulates muscle mass. GDF8 is held in a latent state through interactions with its N-terminal prodomain. GDF8, like numerous TGF-β family members, is a disulfidelinked dimer that is synthesized as a precursor protein which requires cleavage by a furin-like protease to yield an N-terminal prodomain and a C-terminal mature, signaling domain.
Synonyms Myostatin; GDF8; Latent GDF8; Mstn

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.