IL-9, His, Human
IL-9, His, Human

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is < 0.3 ng/mL (QC Test).

IL-9, His, Human

Immobilized IL-9, His, Human, His Tag at 0.5 μg /ml (100 μl /well) on the plate. Dose response curve for Anti-IL-9 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.

IL-9, His, Human

The purity of IL-9, His, Human is greater than 95% as determined by SEC-HPLC.

IL-9, His, Human

IL-9, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-9, His, Human

IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. In this review, we focus on the role of Th9 and IL-9-producing cells in allergic asthma.
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Product Introduction
Species Human
Protein Construction
IL-9 (Gln19-Ile144)
Accession # P15248		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 15.3 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 150mM NaCl (pH 7.5).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IL-9, His, Human
    • IL-9, His, Human

    Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is < 0.3 ng/mL (QC Test).

  • IL-9, His, Human
    • IL-9, His, Human

    Immobilized IL-9, His, Human, His Tag at 0.5 μg /ml (100 μl /well) on the plate. Dose response curve for Anti-IL-9 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.

  • IL-9, His, Human
    • IL-9, His, Human

    The purity of IL-9, His, Human is greater than 95% as determined by SEC-HPLC.

  • IL-9, His, Human
    • IL-9, His, Human

    IL-9, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background IL-9 is a pleiotropic cytokine that influences various distinct functions of different target cells such as T cells, B cells, mast cells and airway epithelial cells by activating STAT1, STAT3 and STAT5. Because of its pleiotropic functions, IL-9 has been demonstrated to be involved in several diseases, such as cancer, autoimmunity and other pathogen-mediated immune-regulated diseases. In this review, we focus on the role of Th9 and IL-9-producing cells in allergic asthma.
Synonyms Interleukin-9; IL-9; Cytokine P40; T-cell growth factor P40; HP40; P40

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.