IL-12B/p40/NKSF2, His, Human
IL-12B/p40/NKSF2, His, Human

Immobilized Human IL-12B, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.12 μg/ml determined by ELISA.

IL-12B/p40/NKSF2, His, Human

IL-12B/p40/NKSF2, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IL-12B/p40/NKSF2, His, Human

Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
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Product Introduction
Species Human
Protein Construction
IL-12B/p40/NKSF2 (Ile23-Ser328)_x000D_
Accession # P29460		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IL-12B/p40/NKSF2, His, Human at 5μg/ml (100μl/well) on the plate can bind Human IL­12 R beta 1, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 35.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IL-12B/p40/NKSF2, His, Human
    • IL-12B/p40/NKSF2, His, Human

    Immobilized Human IL-12B, His Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.12 μg/ml determined by ELISA.

  • IL-12B/p40/NKSF2, His, Human
    • IL-12B/p40/NKSF2, His, Human

    IL-12B/p40/NKSF2, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.
Synonyms IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.