IL-12, His & Avi, Human

IL-12, His & Avi, Human

Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Z05429
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Product Introduction
Species Human
Protein Construction
IL-12 [Ile23-Ser328 (IL-12B) & Arg23-Ser219 (IL-12A)]
Accession # P29460 (IL-12B) & P29459 (IL-12A)		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by SEC­HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IL-12, His & Avi, Human at 1μg/ml (100μl/well) on the plate can bind Anti­IL­12 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 34.7 kDa(IL-12B) & 25.5 kDa(IL-12A)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 40-45 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background Interleukin 12 (IL-12) is a pleiotropic cytokine that plays an essential role in Th1-type immune response against cancer, a condition where cells in a particular part of the body grow and reproduce uncontrollably.
Synonyms CLMF; CLMF2; IL-12A; IL-12B; IL12; IL12 p70; IMD28; IMD29; NFSK; NKSF1; NKSF2; P35; IL-12 subunit p35; IL12A; interleukin 12

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.