IGFI hFc Chimera, Human
IGFI HFc Chimera, Human

Immobilized Human IGF1R, His Tag at 5 μg /ml(100 μl/well) on the plate. Dose response curve for IGFI hFc Chimera, Human, hFc Tag with the EC50 of 0.68 μg /ml determined by ELISA.

IGFI HFc Chimera, Human

The purity of IGFI hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

IGFI HFc Chimera, Human

IGFI hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IGFI HFc Chimera, Human

Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind IGFI hFc Chimera, Human, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).

IGFI hFc Chimera, Human

The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.
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Product Introduction
Species Human
Protein Construction
hFc IGFI (Gly49-Ala118)
Accession # P05019-1
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 33.18 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 38-43 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.

Examples
  • IGFI HFc Chimera, Human
    • IGFI HFc Chimera, Human

    Immobilized Human IGF1R, His Tag at 5 μg /ml(100 μl/well) on the plate. Dose response curve for IGFI hFc Chimera, Human, hFc Tag with the EC50 of 0.68 μg /ml determined by ELISA.

  • IGFI HFc Chimera, Human
    • IGFI HFc Chimera, Human

    The purity of IGFI hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • IGFI HFc Chimera, Human
    • IGFI HFc Chimera, Human

    IGFI hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IGFI HFc Chimera, Human
    • IGFI HFc Chimera, Human

    Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind IGFI hFc Chimera, Human, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).


Background
Target Background The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.
Synonyms IGF-I; MGF; IBP1; IGF1A;

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.