IGFBP-3, His, Human
IGFBP-3, His, Human

Immobilized IGFBP-3, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.20 μg/ml determined by ELISA.

IGFBP-3, His, Human

IGFBP-3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

IGFBP-3, His, Human

IGFBP-3, His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).

IGFBP-3, His, Human

Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
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Product Introduction
Species Human
Protein Construction
IGFBP-3 (Gly28-Lys291)
Accession # AAH18962		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized IGFBP-3, His, Human at 2μg/ml (100μl/well) on the plate can bind Human IGF­II, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 29.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • IGFBP-3, His, Human
    • IGFBP-3, His, Human

    Immobilized IGFBP-3, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.20 μg/ml determined by ELISA.

  • IGFBP-3, His, Human
    • IGFBP-3, His, Human

    IGFBP-3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • IGFBP-3, His, Human
    • IGFBP-3, His, Human

    IGFBP-3, His, Human, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).


Background
Target Background Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer.
Synonyms IBP3; IGFBP3; IGFBP-3; BP-53

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.