ICOS/CD278 hFc Chimera, Human
ICOS/CD278 HFc Chimera, Human

Immobilized Human B7-H2, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for ICOS/CD278 hFc Chimera, Human, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.

ICOS/CD278 HFc Chimera, Human

The purity of ICOS/CD278 hFc Chimera, Human is greater than 90% as determined by SEC-HPLC.

ICOS/CD278 HFc Chimera, Human

ICOS/CD278 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ICOS/CD278 HFc Chimera, Human

ICOS/CD278 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).

ICOS/CD278 hFc Chimera, Human

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
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Product Introduction
Species Human
Protein Construction
ICOS/CD278 (Glu21-Phe141)
Accession # Q9Y6W8-1		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 90% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human B7­H2, His Tag at 1μg/ml (100μl/Well) on the plate can bind ICOS/CD278 hFc Chimera, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 40.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • ICOS/CD278 HFc Chimera, Human
    • ICOS/CD278 HFc Chimera, Human

    Immobilized Human B7-H2, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for ICOS/CD278 hFc Chimera, Human, hFc Tag with the EC50 of 10.9ng/ml determined by ELISA.

  • ICOS/CD278 HFc Chimera, Human
    • ICOS/CD278 HFc Chimera, Human

    The purity of ICOS/CD278 hFc Chimera, Human is greater than 90% as determined by SEC-HPLC.

  • ICOS/CD278 HFc Chimera, Human
    • ICOS/CD278 HFc Chimera, Human

    ICOS/CD278 hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • ICOS/CD278 HFc Chimera, Human
    • ICOS/CD278 HFc Chimera, Human

    ICOS/CD278 hFc Chimera, Human, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus B7-H2, His Tag with an affinity constant of 5.42 nM as determined in SPR assay (Biacore T200).


Background
Target Background Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.