ICOS/CD278 (Q50A) hFc Chimera, Human
ICOS/CD278 (Q50A) HFc Chimera, Human

The purity of ICOS/CD278 (Q50A) hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

ICOS/CD278 (Q50A) HFc Chimera, Human

ICOS/CD278 (Q50A) hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ICOS/CD278 (Q50A) HFc Chimera, Human

ICOS/CD278 (Q50A) hFc Chimera, Human, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).

ICOS/CD278 (Q50A) hFc Chimera, Human

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
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Product Introduction
Species Human
Protein Construction
ICOS/CD278 (Q50A) (Glu21-Phe141)
Accession # Q9Y6W8-1		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity ICOS/CD278 (Q50A) hFc Chimera, Human captured on CM5 Chip via Protein A can bind Human B7­H2, His Tag in SPR assay (Biacore T200). Test result was comparable to standard batch
Expression System HEK293
Theoretical Molecular Weight 40.45 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • ICOS/CD278 (Q50A) HFc Chimera, Human
    • ICOS/CD278 (Q50A) HFc Chimera, Human

    The purity of ICOS/CD278 (Q50A) hFc Chimera, Human is greater than 95% as determined by SEC-HPLC.

  • ICOS/CD278 (Q50A) HFc Chimera, Human
    • ICOS/CD278 (Q50A) HFc Chimera, Human

    ICOS/CD278 (Q50A) hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • ICOS/CD278 (Q50A) HFc Chimera, Human
    • ICOS/CD278 (Q50A) HFc Chimera, Human

    ICOS/CD278 (Q50A) hFc Chimera, Human, hFc Tag (Q50A) captured on CM5 Chip via Protein A can bind Human B7-H2, His Tag with an affinity constant of 41.98 nM as determined in SPR assay (Biacore T200).


Background
Target Background Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.