ICOS/CD278 (C136S, C137S), His, Human
ICOS/CD278 (C136S, C137S), His, Human

Immobilized ICOS/CD278 (C136S, C137S), His, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.

ICOS/CD278 (C136S, C137S), His, Human

ICOS/CD278 (C136S, C137S), His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

ICOS/CD278 (C136S, C137S), His, Human

Human B7-H2, hFc Tag captured on Protein A chip, can bind ICOS/CD278 (C136S, C137S), His, Human , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).

ICOS/CD278 (C136S, C137S), His, Human

Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
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Product Introduction
Species Human
Protein Construction
ICOS/CD278 (C136S, C137S) [Glu21-Phe141 (C136S,C137S)]
Accession # Q9Y6W8-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
> 95% as determined by HPLC
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized ICOS/CD278 (C136S, C137S), His, Human at 5μg/ml (100μl/Well) on the plate can bind Human B7­H2, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 14.8 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • ICOS/CD278 (C136S, C137S), His, Human
    • ICOS/CD278 (C136S, C137S), His, Human

    Immobilized ICOS/CD278 (C136S, C137S), His, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.

  • ICOS/CD278 (C136S, C137S), His, Human
    • ICOS/CD278 (C136S, C137S), His, Human

    ICOS/CD278 (C136S, C137S), His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

  • ICOS/CD278 (C136S, C137S), His, Human
    • ICOS/CD278 (C136S, C137S), His, Human

    Human B7-H2, hFc Tag captured on Protein A chip, can bind ICOS/CD278 (C136S, C137S), His, Human , His Tag with an affinity constant of 40.2nM as determined in a SPR assay (Biacore T200).


Background
Target Background Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.
Synonyms AILIM; CD278; CRP-1; CVID1; ICOS; MGC39850

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.