HGF R/c-MET hFc Chimera, Human
HGF R/c-MET HFc Chimera, Human

Immobilized Human HGF, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for HGF R/c-MET hFc Chimera, Human, hFc Tag with the EC50 of 7.0 ng/ml determined by ELISA.

HGF R/c-MET HFc Chimera, Human

HGF R/c-MET hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

HGF R/c-MET hFc Chimera, Human

c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
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Product Introduction
Species Human
Protein Construction
HGF R/c-MET (Glu25-Thr932)
Accession # P08581-1		 hFc
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Human HGF, His Tag at 2μg/ml (100μl/Well) on the plate can bind HGF R/c-MET hFc Chimera, Human. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 32.5 kDa (α chain) and 95.9 kDa (β chain Fc chimera)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-55 kDa and 100-120 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Examples
  • HGF R/c-MET HFc Chimera, Human
    • HGF R/c-MET HFc Chimera, Human

    Immobilized Human HGF, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for HGF R/c-MET hFc Chimera, Human, hFc Tag with the EC50 of 7.0 ng/ml determined by ELISA.

  • HGF R/c-MET HFc Chimera, Human
    • HGF R/c-MET HFc Chimera, Human

    HGF R/c-MET hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.


Background
Target Background c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGF R), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.
Synonyms MET; oncogene MET; HGF R; HGF/SF receptor; AUTS9; cMET; Met (c-Met); RCCP2; SF receptor

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.