HGF, His, Human

HGF, His, Human

Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
Z05375
¥59,694.00

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Product Introduction
Species Human
Protein Construction
HGF (Gln32-Ser728)
Accession # P14210-1		 His
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
> 95% as determined by HPLC
Endotoxin Level Less than 0.1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized HGF, His, Human at 2μg/ml (100μl/Well) on the plate can bind Human HGF R, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 79.6 kDa (after cleavage of the signal peptide)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.

Background
Target Background Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
Synonyms Hepatopoietin-A; SF; HPTA; HGF; DFNB39; F-TCF; F-TCFB; HGFB

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.